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Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.

Ladinig A, Gerner W, Saalmüller A, Lunney JK, Ashley C, Harding JC - Vet. Res. (2014)

Bottom Line: Our data also show a greater decrease of naïve B cells, T-helper cells and cytolytic T cells than their respective effector or memory counterparts.Absolute numbers of T cells and γδ T cells were negatively associated with PRRSv RNA concentration in gilt serum over time.Although many questions regarding the immune responses remain unanswered, these findings provide insight and clues that may help reduce the impact of PRRSv in pregnant gilts.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada. andrea.ladinig@vetmeduni.ac.at.

ABSTRACT
In spite of more than two decades of extensive research, the understanding of porcine reproductive and respiratory syndrome virus (PRRSv) immunity is still incomplete. A PRRSv infection of the late term pregnant female can result in abortions, early farrowings, fetal death, and the birth of weak, congenitally infected piglets. The objectives of the present study were to investigate changes in peripheral blood mononuclear cell populations in third trimester pregnant females infected with type 2 PRRSv (NVSL 97-7895) and to analyze potential relationships with viral load and fetal mortality rate. PRRSv infection caused a massive, acute drop in total leukocyte counts affecting all PBMC populations by two days post infection. Except for B cells, cell counts started to rebound by day six post infection. Our data also show a greater decrease of naïve B cells, T-helper cells and cytolytic T cells than their respective effector or memory counterparts. Absolute numbers of T cells and γδ T cells were negatively associated with PRRSv RNA concentration in gilt serum over time. Additionally, absolute numbers of T helper cells may be predictive of fetal mortality rate. The preceding three leukocyte populations may therefore be predictive of PRRSv-related pathological outcomes in pregnant gilts. Although many questions regarding the immune responses remain unanswered, these findings provide insight and clues that may help reduce the impact of PRRSv in pregnant gilts.

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Related in: MedlinePlus

Changes in T cells in response to PRRSv infection in pregnant gilts. A)Dot plots: The gating strategy for T cells (CD3+) is demonstrated using representative data from gilt #53. B)Line chart: Changes in absolute numbers (mean ± SD) of T cells are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days.
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Fig5: Changes in T cells in response to PRRSv infection in pregnant gilts. A)Dot plots: The gating strategy for T cells (CD3+) is demonstrated using representative data from gilt #53. B)Line chart: Changes in absolute numbers (mean ± SD) of T cells are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days.

Mentions: Total T cells were identified by a CD3+ phenotype (Figure 5A). Their absolute numbers decreased significantly in INOC compared to CTRL gilts on 2 dpi (P < 0.001), and trended lower on 6 dpi (P = 0.013) (Figure 5B). Three subpopulations of T cells, namely γδ T cells, T helper cells, and cytolytic T cells (CTLs), were analyzed in more detail.Figure 5


Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome.

Ladinig A, Gerner W, Saalmüller A, Lunney JK, Ashley C, Harding JC - Vet. Res. (2014)

Changes in T cells in response to PRRSv infection in pregnant gilts. A)Dot plots: The gating strategy for T cells (CD3+) is demonstrated using representative data from gilt #53. B)Line chart: Changes in absolute numbers (mean ± SD) of T cells are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265508&req=5

Fig5: Changes in T cells in response to PRRSv infection in pregnant gilts. A)Dot plots: The gating strategy for T cells (CD3+) is demonstrated using representative data from gilt #53. B)Line chart: Changes in absolute numbers (mean ± SD) of T cells are presented from 111 INOC and 19 CTRL gilts over time. P-values indicate significant differences between INOC and CTRL gilts on individual days.
Mentions: Total T cells were identified by a CD3+ phenotype (Figure 5A). Their absolute numbers decreased significantly in INOC compared to CTRL gilts on 2 dpi (P < 0.001), and trended lower on 6 dpi (P = 0.013) (Figure 5B). Three subpopulations of T cells, namely γδ T cells, T helper cells, and cytolytic T cells (CTLs), were analyzed in more detail.Figure 5

Bottom Line: Our data also show a greater decrease of naïve B cells, T-helper cells and cytolytic T cells than their respective effector or memory counterparts.Absolute numbers of T cells and γδ T cells were negatively associated with PRRSv RNA concentration in gilt serum over time.Although many questions regarding the immune responses remain unanswered, these findings provide insight and clues that may help reduce the impact of PRRSv in pregnant gilts.

View Article: PubMed Central - PubMed

Affiliation: Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada. andrea.ladinig@vetmeduni.ac.at.

ABSTRACT
In spite of more than two decades of extensive research, the understanding of porcine reproductive and respiratory syndrome virus (PRRSv) immunity is still incomplete. A PRRSv infection of the late term pregnant female can result in abortions, early farrowings, fetal death, and the birth of weak, congenitally infected piglets. The objectives of the present study were to investigate changes in peripheral blood mononuclear cell populations in third trimester pregnant females infected with type 2 PRRSv (NVSL 97-7895) and to analyze potential relationships with viral load and fetal mortality rate. PRRSv infection caused a massive, acute drop in total leukocyte counts affecting all PBMC populations by two days post infection. Except for B cells, cell counts started to rebound by day six post infection. Our data also show a greater decrease of naïve B cells, T-helper cells and cytolytic T cells than their respective effector or memory counterparts. Absolute numbers of T cells and γδ T cells were negatively associated with PRRSv RNA concentration in gilt serum over time. Additionally, absolute numbers of T helper cells may be predictive of fetal mortality rate. The preceding three leukocyte populations may therefore be predictive of PRRSv-related pathological outcomes in pregnant gilts. Although many questions regarding the immune responses remain unanswered, these findings provide insight and clues that may help reduce the impact of PRRSv in pregnant gilts.

Show MeSH
Related in: MedlinePlus