Limits...
The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating brain lesion.

Bennett HM, Mok HP, Gkrania-Klotsas E, Tsai IJ, Stanley EJ, Antoun NM, Coghlan A, Harsha B, Traini A, Ribeiro DM, Steinbiss S, Lucas SB, Allinson KS, Price SJ, Santarius TS, Carmichael AJ, Chiodini PL, Holroyd N, Dean AF, Berriman M - Genome Biol. (2014)

Bottom Line: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm.Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.From a single clinical case we have begun to sketch a picture of the characteristics of these organisms.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From a rare cerebral case presented at a clinic in the UK, DNA was recovered from a biopsy sample and used to determine the causative species as Spirometra erinaceieuropaei through sequencing of the cox1 gene. From the same DNA, we have produced a draft genome, the first of its kind for this species, and used it to perform a comparative genomics analysis and to investigate known and potential tapeworm drug targets in this tapeworm.

Results: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm. Through investigation of β-tubulin genes, we predict that S. erinaceieuropaei larvae are insensitive to the tapeworm drug albendazole. We find that many putative tapeworm drug targets are also present in S. erinaceieuropaei, allowing possible cross application of new drugs. In comparison to other sequenced tapeworm species we observe expansion of protease classes, and of Kuntiz-type protease inhibitors. Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.

Conclusions: The S. erinaceieuropaei genome begins to give us insight into an order of tapeworms previously uncharacterized at the genome-wide level. From a single clinical case we have begun to sketch a picture of the characteristics of these organisms. Finally, our work represents a significant technological achievement as we present a draft genome sequence of a rare tapeworm, and from a small amount of starting material.

Show MeSH

Related in: MedlinePlus

Cross-species comparison of protease and protease inhibitor classes. Protease and protease inhibitors by MEROPS classification in Echinococcus granulosus (green), E. multilocularis (orange) and S. erinaceieuropaei (purple) ordered alphabetically. In all species there is a large number of I02 class members, representing Kunitz-type protease inhibitors. The M17 class consists of leucyl aminopeptidases and the SO1A and S28 classes are serine endopeptidases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4265353&req=5

Fig5: Cross-species comparison of protease and protease inhibitor classes. Protease and protease inhibitors by MEROPS classification in Echinococcus granulosus (green), E. multilocularis (orange) and S. erinaceieuropaei (purple) ordered alphabetically. In all species there is a large number of I02 class members, representing Kunitz-type protease inhibitors. The M17 class consists of leucyl aminopeptidases and the SO1A and S28 classes are serine endopeptidases.

Mentions: Proteases and protease inhibitors are well known for their importance in host-parasite relationships [31-33]. Using InterProScan 5 we identified 302 sequences that contained predicted proteases or protease inhibitor domains. Using the MEROPS databases of proteases and protease inhibitors [34], we classified 242 of these genes and found the most abundant to be inhibitors of serine proteases (Figure 5). Interestingly, two classes of proteases appeared to be considerably expanded in comparison to Echinococcus spp.: both the M17 (amino-terminal leucyl aminopeptidases) and the serine endopeptidase classes S1A (chymotrypsin A-like) and S28 (lysosomal Pro-Xaa carboxypeptidase-like).Figure 5


The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating brain lesion.

Bennett HM, Mok HP, Gkrania-Klotsas E, Tsai IJ, Stanley EJ, Antoun NM, Coghlan A, Harsha B, Traini A, Ribeiro DM, Steinbiss S, Lucas SB, Allinson KS, Price SJ, Santarius TS, Carmichael AJ, Chiodini PL, Holroyd N, Dean AF, Berriman M - Genome Biol. (2014)

Cross-species comparison of protease and protease inhibitor classes. Protease and protease inhibitors by MEROPS classification in Echinococcus granulosus (green), E. multilocularis (orange) and S. erinaceieuropaei (purple) ordered alphabetically. In all species there is a large number of I02 class members, representing Kunitz-type protease inhibitors. The M17 class consists of leucyl aminopeptidases and the SO1A and S28 classes are serine endopeptidases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265353&req=5

Fig5: Cross-species comparison of protease and protease inhibitor classes. Protease and protease inhibitors by MEROPS classification in Echinococcus granulosus (green), E. multilocularis (orange) and S. erinaceieuropaei (purple) ordered alphabetically. In all species there is a large number of I02 class members, representing Kunitz-type protease inhibitors. The M17 class consists of leucyl aminopeptidases and the SO1A and S28 classes are serine endopeptidases.
Mentions: Proteases and protease inhibitors are well known for their importance in host-parasite relationships [31-33]. Using InterProScan 5 we identified 302 sequences that contained predicted proteases or protease inhibitor domains. Using the MEROPS databases of proteases and protease inhibitors [34], we classified 242 of these genes and found the most abundant to be inhibitors of serine proteases (Figure 5). Interestingly, two classes of proteases appeared to be considerably expanded in comparison to Echinococcus spp.: both the M17 (amino-terminal leucyl aminopeptidases) and the serine endopeptidase classes S1A (chymotrypsin A-like) and S28 (lysosomal Pro-Xaa carboxypeptidase-like).Figure 5

Bottom Line: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm.Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.From a single clinical case we have begun to sketch a picture of the characteristics of these organisms.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From a rare cerebral case presented at a clinic in the UK, DNA was recovered from a biopsy sample and used to determine the causative species as Spirometra erinaceieuropaei through sequencing of the cox1 gene. From the same DNA, we have produced a draft genome, the first of its kind for this species, and used it to perform a comparative genomics analysis and to investigate known and potential tapeworm drug targets in this tapeworm.

Results: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm. Through investigation of β-tubulin genes, we predict that S. erinaceieuropaei larvae are insensitive to the tapeworm drug albendazole. We find that many putative tapeworm drug targets are also present in S. erinaceieuropaei, allowing possible cross application of new drugs. In comparison to other sequenced tapeworm species we observe expansion of protease classes, and of Kuntiz-type protease inhibitors. Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.

Conclusions: The S. erinaceieuropaei genome begins to give us insight into an order of tapeworms previously uncharacterized at the genome-wide level. From a single clinical case we have begun to sketch a picture of the characteristics of these organisms. Finally, our work represents a significant technological achievement as we present a draft genome sequence of a rare tapeworm, and from a small amount of starting material.

Show MeSH
Related in: MedlinePlus