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The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating brain lesion.

Bennett HM, Mok HP, Gkrania-Klotsas E, Tsai IJ, Stanley EJ, Antoun NM, Coghlan A, Harsha B, Traini A, Ribeiro DM, Steinbiss S, Lucas SB, Allinson KS, Price SJ, Santarius TS, Carmichael AJ, Chiodini PL, Holroyd N, Dean AF, Berriman M - Genome Biol. (2014)

Bottom Line: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm.Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.From a single clinical case we have begun to sketch a picture of the characteristics of these organisms.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From a rare cerebral case presented at a clinic in the UK, DNA was recovered from a biopsy sample and used to determine the causative species as Spirometra erinaceieuropaei through sequencing of the cox1 gene. From the same DNA, we have produced a draft genome, the first of its kind for this species, and used it to perform a comparative genomics analysis and to investigate known and potential tapeworm drug targets in this tapeworm.

Results: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm. Through investigation of β-tubulin genes, we predict that S. erinaceieuropaei larvae are insensitive to the tapeworm drug albendazole. We find that many putative tapeworm drug targets are also present in S. erinaceieuropaei, allowing possible cross application of new drugs. In comparison to other sequenced tapeworm species we observe expansion of protease classes, and of Kuntiz-type protease inhibitors. Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.

Conclusions: The S. erinaceieuropaei genome begins to give us insight into an order of tapeworms previously uncharacterized at the genome-wide level. From a single clinical case we have begun to sketch a picture of the characteristics of these organisms. Finally, our work represents a significant technological achievement as we present a draft genome sequence of a rare tapeworm, and from a small amount of starting material.

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Sequential imaging over a 4-year period identifies migrating lesions. Sequential imaging over 4-year period: July 2008 to June 2012. All images are coronal T1 scans post gadolinium. The shifting white arrow, from right to left hemispheres, depicts the migration pattern of a cluster of ring-enhancing lesions.
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Fig2: Sequential imaging over a 4-year period identifies migrating lesions. Sequential imaging over 4-year period: July 2008 to June 2012. All images are coronal T1 scans post gadolinium. The shifting white arrow, from right to left hemispheres, depicts the migration pattern of a cluster of ring-enhancing lesions.

Mentions: A series of MRI images in the ensuing four years demonstrated contralateral gradual migration of the multiloculate lesions from the right hemisphere through the thalamus (Figure 2). Throughout the disease process, the lesion had moved at least 5 cm through the brain. A second biopsy, from the left thalamus, showed granulomatous inflammation, focal necrosis and an approximately 1 cm ribbon-shaped cestode larval worm without mouthparts or hooklets. With the pathognominic morphology of a sparganum, it was so diagnosed at the Department of Histopathology, St Thomas’ Hospital, and the Department of Clinical Parasitology, Hospital for Tropical Diseases (Figure 3). Immediately post-operation, the patient was given albendazole and is now systemically well.Figure 2


The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating brain lesion.

Bennett HM, Mok HP, Gkrania-Klotsas E, Tsai IJ, Stanley EJ, Antoun NM, Coghlan A, Harsha B, Traini A, Ribeiro DM, Steinbiss S, Lucas SB, Allinson KS, Price SJ, Santarius TS, Carmichael AJ, Chiodini PL, Holroyd N, Dean AF, Berriman M - Genome Biol. (2014)

Sequential imaging over a 4-year period identifies migrating lesions. Sequential imaging over 4-year period: July 2008 to June 2012. All images are coronal T1 scans post gadolinium. The shifting white arrow, from right to left hemispheres, depicts the migration pattern of a cluster of ring-enhancing lesions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265353&req=5

Fig2: Sequential imaging over a 4-year period identifies migrating lesions. Sequential imaging over 4-year period: July 2008 to June 2012. All images are coronal T1 scans post gadolinium. The shifting white arrow, from right to left hemispheres, depicts the migration pattern of a cluster of ring-enhancing lesions.
Mentions: A series of MRI images in the ensuing four years demonstrated contralateral gradual migration of the multiloculate lesions from the right hemisphere through the thalamus (Figure 2). Throughout the disease process, the lesion had moved at least 5 cm through the brain. A second biopsy, from the left thalamus, showed granulomatous inflammation, focal necrosis and an approximately 1 cm ribbon-shaped cestode larval worm without mouthparts or hooklets. With the pathognominic morphology of a sparganum, it was so diagnosed at the Department of Histopathology, St Thomas’ Hospital, and the Department of Clinical Parasitology, Hospital for Tropical Diseases (Figure 3). Immediately post-operation, the patient was given albendazole and is now systemically well.Figure 2

Bottom Line: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm.Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.From a single clinical case we have begun to sketch a picture of the characteristics of these organisms.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From a rare cerebral case presented at a clinic in the UK, DNA was recovered from a biopsy sample and used to determine the causative species as Spirometra erinaceieuropaei through sequencing of the cox1 gene. From the same DNA, we have produced a draft genome, the first of its kind for this species, and used it to perform a comparative genomics analysis and to investigate known and potential tapeworm drug targets in this tapeworm.

Results: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm. Through investigation of β-tubulin genes, we predict that S. erinaceieuropaei larvae are insensitive to the tapeworm drug albendazole. We find that many putative tapeworm drug targets are also present in S. erinaceieuropaei, allowing possible cross application of new drugs. In comparison to other sequenced tapeworm species we observe expansion of protease classes, and of Kuntiz-type protease inhibitors. Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification.

Conclusions: The S. erinaceieuropaei genome begins to give us insight into an order of tapeworms previously uncharacterized at the genome-wide level. From a single clinical case we have begun to sketch a picture of the characteristics of these organisms. Finally, our work represents a significant technological achievement as we present a draft genome sequence of a rare tapeworm, and from a small amount of starting material.

Show MeSH
Related in: MedlinePlus