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In vitro antimicrobial, antioxidant and cytotoxic properties of Streptomyces lavendulae strain SCA5.

Saravana Kumar P, Al-Dhabi NA, Duraipandiyan V, Balachandran C, Praveen Kumar P, Ignacimuthu S - BMC Microbiol. (2014)

Bottom Line: The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC50 838.83 ± 1.18 μg/ml).The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.The results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Actinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities. The aim of the present study was to evaluate the antimicrobial, cytotoxic and antioxidant properties of Streptomyces lavendulae strain SCA5.

Results: The ethyl acetate extract of SCA5 broth (EA-SCA5) showed antimicrobial activity with MIC value of 31.25 μg/ml. EA-SCA5 showed good antioxidant potential by scavenging 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 507.61 ± 0.66 μg/ml), hydroxyl radical (IC50 617.84 ± 0.57 μg/ml), nitric oxide (IC50 730.92 ± 0.81 μg/ml) and superoxide anion radical (IC50 864.71 ± 1.15 μg/ml). The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC50 838.83 ± 1.18 μg/ml). The total phenolic content of SCA5 was 577.12 mg of GAE equivalents/gram extract. EA-SCA5 exhibited cytotoxic activity on A549 adenocarcinoma lung cancer cell line. It showed 84.9% activity at 500 μg/ml with IC50 value of 200 μg/ml. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.

Conclusions: The results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.

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Related in: MedlinePlus

TLC profile of the active crude from soilStreptomyces lavendulaestrain SCA5. a. Viewed under normal white light. b. Viewed under UV 366 nm. c. Derivatization with 10% alcoholic sulphuric acid. d. Exposure to iodine vapour. e. 0.01% ferric chloride. f. 0.01% alcoholic NaOH.
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Fig4: TLC profile of the active crude from soilStreptomyces lavendulaestrain SCA5. a. Viewed under normal white light. b. Viewed under UV 366 nm. c. Derivatization with 10% alcoholic sulphuric acid. d. Exposure to iodine vapour. e. 0.01% ferric chloride. f. 0.01% alcoholic NaOH.

Mentions: TLC profile of the active ethyl acetate extract is given in Figure 4 with various methods of visualization. As shown in Figures 4C and D the extract contained phenol and quinone.Figure 4


In vitro antimicrobial, antioxidant and cytotoxic properties of Streptomyces lavendulae strain SCA5.

Saravana Kumar P, Al-Dhabi NA, Duraipandiyan V, Balachandran C, Praveen Kumar P, Ignacimuthu S - BMC Microbiol. (2014)

TLC profile of the active crude from soilStreptomyces lavendulaestrain SCA5. a. Viewed under normal white light. b. Viewed under UV 366 nm. c. Derivatization with 10% alcoholic sulphuric acid. d. Exposure to iodine vapour. e. 0.01% ferric chloride. f. 0.01% alcoholic NaOH.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265329&req=5

Fig4: TLC profile of the active crude from soilStreptomyces lavendulaestrain SCA5. a. Viewed under normal white light. b. Viewed under UV 366 nm. c. Derivatization with 10% alcoholic sulphuric acid. d. Exposure to iodine vapour. e. 0.01% ferric chloride. f. 0.01% alcoholic NaOH.
Mentions: TLC profile of the active ethyl acetate extract is given in Figure 4 with various methods of visualization. As shown in Figures 4C and D the extract contained phenol and quinone.Figure 4

Bottom Line: The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC50 838.83 ± 1.18 μg/ml).The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.The results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Actinomycetes are Gram-positive, often filamentous, bacteria known for their unsurpassed capacity for the production of secondary metabolites with diverse biological activities. The aim of the present study was to evaluate the antimicrobial, cytotoxic and antioxidant properties of Streptomyces lavendulae strain SCA5.

Results: The ethyl acetate extract of SCA5 broth (EA-SCA5) showed antimicrobial activity with MIC value of 31.25 μg/ml. EA-SCA5 showed good antioxidant potential by scavenging 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 507.61 ± 0.66 μg/ml), hydroxyl radical (IC50 617.84 ± 0.57 μg/ml), nitric oxide (IC50 730.92 ± 0.81 μg/ml) and superoxide anion radical (IC50 864.71 ± 1.15 μg/ml). The EA-SCA5 also showed strong suppressive effect on rat liver lipid peroxidation (IC50 838.83 ± 1.18 μg/ml). The total phenolic content of SCA5 was 577.12 mg of GAE equivalents/gram extract. EA-SCA5 exhibited cytotoxic activity on A549 adenocarcinoma lung cancer cell line. It showed 84.9% activity at 500 μg/ml with IC50 value of 200 μg/ml. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of one major bioactive compound actinomycin C2.

Conclusions: The results of this study indicate that the EA-SCA5 could be probed further for isolating some medically useful compounds.

Show MeSH
Related in: MedlinePlus