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Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes.

Effendi Y, Ferro N, Labusch C, Geisler M, Scherer GF - J. Exp. Bot. (2014)

Bottom Line: Red light effects, such as elongation of hypocotyls in constant red (R) and far-red (FR) light, in white light supplemented by FR light simulating shade, and inhibition of gravitropism by R or FR, were all compromised in the complemented lines.The expression of marker genes in seedlings responding to both auxin and shade showed that for both stimuli regulation of marker gene expression was altered after 10-20 min in the wild type and phyB mutant.The rapidity of expression responses provides a framework for the mechanics of functional interaction of ABP1 and phyB to trigger interwoven signalling pathways.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany Al Azhar Indonesia University, Department of Biology, Sisingamangaraja, Jakarta 12110, Indonesia.

No MeSH data available.


Expression of early auxin genes and several PIN genes in 14-day-old light-grown seedlings. Seedlings were either treated with 10 µM IAA in (A) (grey bars: 10min, black bars: 30min) or mock in (B). qRT-PCRs were from three biological replicates with three technical replicates for each gene. Statistical analysis was performed as described by Livak and Schmittgen (2001) and verified using the method of Pfaffl et al. (2002). At t=0min fold expression was set as 1 for the wt in (B). Asterisks indicate significant difference to the wt (* P<0.05; ** P<0.01).
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Figure 3: Expression of early auxin genes and several PIN genes in 14-day-old light-grown seedlings. Seedlings were either treated with 10 µM IAA in (A) (grey bars: 10min, black bars: 30min) or mock in (B). qRT-PCRs were from three biological replicates with three technical replicates for each gene. Statistical analysis was performed as described by Livak and Schmittgen (2001) and verified using the method of Pfaffl et al. (2002). At t=0min fold expression was set as 1 for the wt in (B). Asterisks indicate significant difference to the wt (* P<0.05; ** P<0.01).

Mentions: Seedling experiments were performed on sterile 1% (w/v) agar (growth experiments), 0.5% (w/v) gelrite to stabilize tropism experiments (Santner and Watson, 2006), or liquid (seedlings for RNA extraction) half-strength Murashige and Skoog (MS) medium containing 1% (w/v) sucrose at 22 °C for 10 d or as otherwise indicated (Figs 2 and 3). Experiments were repeated two to three times independently (n=75–90).


Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes.

Effendi Y, Ferro N, Labusch C, Geisler M, Scherer GF - J. Exp. Bot. (2014)

Expression of early auxin genes and several PIN genes in 14-day-old light-grown seedlings. Seedlings were either treated with 10 µM IAA in (A) (grey bars: 10min, black bars: 30min) or mock in (B). qRT-PCRs were from three biological replicates with three technical replicates for each gene. Statistical analysis was performed as described by Livak and Schmittgen (2001) and verified using the method of Pfaffl et al. (2002). At t=0min fold expression was set as 1 for the wt in (B). Asterisks indicate significant difference to the wt (* P<0.05; ** P<0.01).
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265171&req=5

Figure 3: Expression of early auxin genes and several PIN genes in 14-day-old light-grown seedlings. Seedlings were either treated with 10 µM IAA in (A) (grey bars: 10min, black bars: 30min) or mock in (B). qRT-PCRs were from three biological replicates with three technical replicates for each gene. Statistical analysis was performed as described by Livak and Schmittgen (2001) and verified using the method of Pfaffl et al. (2002). At t=0min fold expression was set as 1 for the wt in (B). Asterisks indicate significant difference to the wt (* P<0.05; ** P<0.01).
Mentions: Seedling experiments were performed on sterile 1% (w/v) agar (growth experiments), 0.5% (w/v) gelrite to stabilize tropism experiments (Santner and Watson, 2006), or liquid (seedlings for RNA extraction) half-strength Murashige and Skoog (MS) medium containing 1% (w/v) sucrose at 22 °C for 10 d or as otherwise indicated (Figs 2 and 3). Experiments were repeated two to three times independently (n=75–90).

Bottom Line: Red light effects, such as elongation of hypocotyls in constant red (R) and far-red (FR) light, in white light supplemented by FR light simulating shade, and inhibition of gravitropism by R or FR, were all compromised in the complemented lines.The expression of marker genes in seedlings responding to both auxin and shade showed that for both stimuli regulation of marker gene expression was altered after 10-20 min in the wild type and phyB mutant.The rapidity of expression responses provides a framework for the mechanics of functional interaction of ABP1 and phyB to trigger interwoven signalling pathways.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Herrenhäuser Str. 2, D-30419 Hannover, Germany Al Azhar Indonesia University, Department of Biology, Sisingamangaraja, Jakarta 12110, Indonesia.

No MeSH data available.