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MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.

Konishi M, Donner TJ, Scarpella E, Yanagisawa S - J. Exp. Bot. (2014)

Bottom Line: Although no apparent phenotype of the single dof5.8 mutants was found, phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp, with an increase in the penetrance of the 'rootless' phenotype and a reduction in the number of cotyledons.Furthermore, interestingly, although mp mutants showed reduced vascular pattern complexity in cotyledons, the mp dof5.8 double mutants displayed both more simplex and more complex vascular patterns in individual cotyledons.These results imply that the product of Dof5.8 whose expression is regulated by MP at least in part might be involved in multiple processes controlled by MP.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.

No MeSH data available.


The synergistic effects caused by dof5.8 and arf5-2 mutations. (A) The number of cotyledons in rootless seedlings of arf5-2 and arf5-2 dof5.8 mutants. (B–D) Images of 7-day-old wild-type (B), arf5-2 (C), and arf5-2 dof5.8–1 (D) seedlings. (E, F) Cleared images of arf5-2 dof5.8-1 (E) and Col (F) seedlings. White and red arrowheads in (D, E) indicate true leaves and vascular elements, respectively. ‘hy-like’ and ‘hy’ in (D–F) indicate a hypocotyl-like structure and hypocotyl, respectively. Scale bars=1mm in (B–D) and 0.5mm in (D–F).
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Figure 5: The synergistic effects caused by dof5.8 and arf5-2 mutations. (A) The number of cotyledons in rootless seedlings of arf5-2 and arf5-2 dof5.8 mutants. (B–D) Images of 7-day-old wild-type (B), arf5-2 (C), and arf5-2 dof5.8–1 (D) seedlings. (E, F) Cleared images of arf5-2 dof5.8-1 (E) and Col (F) seedlings. White and red arrowheads in (D, E) indicate true leaves and vascular elements, respectively. ‘hy-like’ and ‘hy’ in (D–F) indicate a hypocotyl-like structure and hypocotyl, respectively. Scale bars=1mm in (B–D) and 0.5mm in (D–F).

Mentions: Another effect of dof5.8 mutations in the arf5-2 mutant was also found. Most of the rootless arf5-2 seedlings (95%) possessed two cotyledons, while far fewer seedlings of rootless arf5-2 dof5.8-1 (10.7%) and arf5-2 dof5.8–2 (18.2%) seedlings possessed two cotyledons (Fig. 5A). Furthermore, considerable numbers of the double mutant seedlings had no cotyledons, although such a phenotype was rarely seen in the single mp mutants. The cotyledon-less seedlings of the double mutants always had a fat hypocotyl-like structure, which was topped with true leaves with trichomes but did not include developed vascular elements (Fig. 5D, E). This result suggests that an interaction between arf5-2 and dof5.8 mutations influenced embryonic development and thus formation of cotyledons.


MONOPTEROS directly activates the auxin-inducible promoter of the Dof5.8 transcription factor gene in Arabidopsis thaliana leaf provascular cells.

Konishi M, Donner TJ, Scarpella E, Yanagisawa S - J. Exp. Bot. (2014)

The synergistic effects caused by dof5.8 and arf5-2 mutations. (A) The number of cotyledons in rootless seedlings of arf5-2 and arf5-2 dof5.8 mutants. (B–D) Images of 7-day-old wild-type (B), arf5-2 (C), and arf5-2 dof5.8–1 (D) seedlings. (E, F) Cleared images of arf5-2 dof5.8-1 (E) and Col (F) seedlings. White and red arrowheads in (D, E) indicate true leaves and vascular elements, respectively. ‘hy-like’ and ‘hy’ in (D–F) indicate a hypocotyl-like structure and hypocotyl, respectively. Scale bars=1mm in (B–D) and 0.5mm in (D–F).
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Figure 5: The synergistic effects caused by dof5.8 and arf5-2 mutations. (A) The number of cotyledons in rootless seedlings of arf5-2 and arf5-2 dof5.8 mutants. (B–D) Images of 7-day-old wild-type (B), arf5-2 (C), and arf5-2 dof5.8–1 (D) seedlings. (E, F) Cleared images of arf5-2 dof5.8-1 (E) and Col (F) seedlings. White and red arrowheads in (D, E) indicate true leaves and vascular elements, respectively. ‘hy-like’ and ‘hy’ in (D–F) indicate a hypocotyl-like structure and hypocotyl, respectively. Scale bars=1mm in (B–D) and 0.5mm in (D–F).
Mentions: Another effect of dof5.8 mutations in the arf5-2 mutant was also found. Most of the rootless arf5-2 seedlings (95%) possessed two cotyledons, while far fewer seedlings of rootless arf5-2 dof5.8-1 (10.7%) and arf5-2 dof5.8–2 (18.2%) seedlings possessed two cotyledons (Fig. 5A). Furthermore, considerable numbers of the double mutant seedlings had no cotyledons, although such a phenotype was rarely seen in the single mp mutants. The cotyledon-less seedlings of the double mutants always had a fat hypocotyl-like structure, which was topped with true leaves with trichomes but did not include developed vascular elements (Fig. 5D, E). This result suggests that an interaction between arf5-2 and dof5.8 mutations influenced embryonic development and thus formation of cotyledons.

Bottom Line: Although no apparent phenotype of the single dof5.8 mutants was found, phenotypic analysis with the mp dof5.8 double mutants revealed that mutations within Dof5.8 enhanced the phenotype of a weak allele of mp, with an increase in the penetrance of the 'rootless' phenotype and a reduction in the number of cotyledons.Furthermore, interestingly, although mp mutants showed reduced vascular pattern complexity in cotyledons, the mp dof5.8 double mutants displayed both more simplex and more complex vascular patterns in individual cotyledons.These results imply that the product of Dof5.8 whose expression is regulated by MP at least in part might be involved in multiple processes controlled by MP.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Center, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.

No MeSH data available.