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Rab geranylgeranyl transferase β subunit is essential for male fertility and tip growth in Arabidopsis.

Gutkowska M, Wnuk M, Nowakowska J, Lichocka M, Stronkowski MM, Swiezewska E - J. Exp. Bot. (2014)

Bottom Line: Here it is shown that mutations in each of the RGTB genes cause a tip growth defect, visible as root hair and pollen tube deformations.Moreover, FM 1-43 styryl dye endocytosis and recycling are affected in the mutant root hairs.Doubly mutated pollen is shrunken, has an abnormal exine structure, and shows strong disorganization of internal membranes, particularly of the endoplasmic reticulum system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland gosiag@ibb.waw.pl.

No MeSH data available.


Related in: MedlinePlus

Male gametophyte defect in the Atrgtb1Atrgtb2 double mutant. (A) Number of seeds per silique, n>20; bars represent mean ±SE. (B) Frequency of germination of seeds from self-pollination of Atrgtb1+/–Atrgtb2–/– plants, n>250. Statistical significance analysed by Student t-test, *P<0.05, ***P<0.001. (C) Siliques of an Atrgtb1+/–Atrgtb2–/– plant after self-pollination; scale bar=1mm. (This figure is available in colour at JXB online.)
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Figure 5: Male gametophyte defect in the Atrgtb1Atrgtb2 double mutant. (A) Number of seeds per silique, n>20; bars represent mean ±SE. (B) Frequency of germination of seeds from self-pollination of Atrgtb1+/–Atrgtb2–/– plants, n>250. Statistical significance analysed by Student t-test, *P<0.05, ***P<0.001. (C) Siliques of an Atrgtb1+/–Atrgtb2–/– plant after self-pollination; scale bar=1mm. (This figure is available in colour at JXB online.)

Mentions: To verify the assumption that the lack of RGT activity is lethal in plants and to investigate the stage on which lethality occurs, genotyping of plants from the F3 generation of the initial crosses, namely the progeny of Atrgtb1+/–Atrgtb2–/– self-pollination, was performed. In F3 progeny of both initial crosses no double mutant homozygotes were found, although 1:2:1 segregation was assumed (Table 1). To check whether the double Atrgtb1–/–Atrgtb2–/– mutation causes embryolethality, the siliques of the Atrgtb1+/–Atrgtb2–/– plants after self-pollination were opened. The number of seeds was only slightly diminished compared with the wt, with a surprisingly low number of unfertilized ovules or dead embryos (Fig. 5). Moreover, the ratio of seed germination and the lethality of young 1-week-old seedlings were the same as in the wt in both F3 progeny lines (Fig. 5A, B).


Rab geranylgeranyl transferase β subunit is essential for male fertility and tip growth in Arabidopsis.

Gutkowska M, Wnuk M, Nowakowska J, Lichocka M, Stronkowski MM, Swiezewska E - J. Exp. Bot. (2014)

Male gametophyte defect in the Atrgtb1Atrgtb2 double mutant. (A) Number of seeds per silique, n>20; bars represent mean ±SE. (B) Frequency of germination of seeds from self-pollination of Atrgtb1+/–Atrgtb2–/– plants, n>250. Statistical significance analysed by Student t-test, *P<0.05, ***P<0.001. (C) Siliques of an Atrgtb1+/–Atrgtb2–/– plant after self-pollination; scale bar=1mm. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4265159&req=5

Figure 5: Male gametophyte defect in the Atrgtb1Atrgtb2 double mutant. (A) Number of seeds per silique, n>20; bars represent mean ±SE. (B) Frequency of germination of seeds from self-pollination of Atrgtb1+/–Atrgtb2–/– plants, n>250. Statistical significance analysed by Student t-test, *P<0.05, ***P<0.001. (C) Siliques of an Atrgtb1+/–Atrgtb2–/– plant after self-pollination; scale bar=1mm. (This figure is available in colour at JXB online.)
Mentions: To verify the assumption that the lack of RGT activity is lethal in plants and to investigate the stage on which lethality occurs, genotyping of plants from the F3 generation of the initial crosses, namely the progeny of Atrgtb1+/–Atrgtb2–/– self-pollination, was performed. In F3 progeny of both initial crosses no double mutant homozygotes were found, although 1:2:1 segregation was assumed (Table 1). To check whether the double Atrgtb1–/–Atrgtb2–/– mutation causes embryolethality, the siliques of the Atrgtb1+/–Atrgtb2–/– plants after self-pollination were opened. The number of seeds was only slightly diminished compared with the wt, with a surprisingly low number of unfertilized ovules or dead embryos (Fig. 5). Moreover, the ratio of seed germination and the lethality of young 1-week-old seedlings were the same as in the wt in both F3 progeny lines (Fig. 5A, B).

Bottom Line: Here it is shown that mutations in each of the RGTB genes cause a tip growth defect, visible as root hair and pollen tube deformations.Moreover, FM 1-43 styryl dye endocytosis and recycling are affected in the mutant root hairs.Doubly mutated pollen is shrunken, has an abnormal exine structure, and shows strong disorganization of internal membranes, particularly of the endoplasmic reticulum system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland gosiag@ibb.waw.pl.

No MeSH data available.


Related in: MedlinePlus