Mutualistic interaction between Salmonella enterica and Aspergillus niger and its effects on Zea mays colonization.
Bottom Line: Aspergillus niger is a ubiquitous fungus that can often be found in soil or associated to plants and microbial consortia.In this work, we have found that this interaction is stable for weeks without a noticeable negative effect on either organism.Strikingly, co-colonization also causes a reduction in plant invasion by S. Typhimurium.
Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, HIM building, Room #1042, Boston, MA, 02115, USA.Show MeSH
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Mentions: Co-incubations took place in either 10 mM potassium phosphate buffer (pH 7) or 10% M9 (v/v) supplemented with 0.01% sucrose (w/v), at 30°C and 130 r.p.m (unless indicated otherwise) for different periods of time prior to the corresponding analysis. Fungal concentration in co-cultures was 1 mycelial microcolony per millilitre and bacterial one was 2 × 107 cells ml−1. In the experiments involving propidium iodide (PI; Figs 3 and 5), mycelia were incubated for 20 min in a freshly made solution at 2.5 μg ml−1 in 10 mM potassium phosphate buffer (pH 7), prior to the analysis. PI is red fluorescent when bound to nucleic acids and membrane impermeant. Therefore, PI is excluded from viable cells and only can penetrate cells when their membrane is compromised so it can be used to identify dead cells (Bjerknes, 1984).
Affiliation: Department of Microbiology and Immunobiology, Harvard Medical School, 77 Avenue Louis Pasteur, HIM building, Room #1042, Boston, MA, 02115, USA.