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A novel α/β-hydrolase gene IbMas enhances salt tolerance in transgenic sweetpotato.

Liu D, Wang L, Zhai H, Song X, He S, Liu Q - PLoS ONE (2014)

Bottom Line: Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type.Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants.H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing, China.

ABSTRACT
Salt stress is one of the major environmental stresses in agriculture worldwide and affects crop productivity and quality. The development of crops with elevated levels of salt tolerance is therefore highly desirable. In the present study, a novel maspardin gene, named IbMas, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbMas contains maspardin domain and belongs to α/β-hydrolase superfamily. Expression of IbMas was up-regulated in sweetpotato under salt stress and ABA treatment. The IbMas-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbMas up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes, under salt stress. These findings suggest that overexpression of IbMas enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.

No MeSH data available.


Related in: MedlinePlus

Relative expression level of IbMas and salt stress responsive genes in the IbMas-overexpressing sweetpotato plants.P5CS: pyrroline-5-carboxylate synthase; P5CR: pyrroline-5-carboxylate reductase; SOD: superoxide dismutase; psbA: encoding D1 protein; PRK: phosphoribulokinase (PRKase). The salt-tolerant transgenic plants (L52, L99, L102 and L101) and wild-type plant (WT) in vitro-grown plants were submerged for 12 h in 1/2 MS medium containing 0 and 200 mM NaCl, respectively. The sweetpotato β-actin gene was used as an internal control. The results are expressed as relative values based on WT grown under control condition as reference sample set to 1.0. Data are presented as means ± SE (n = 3). * and ** indicate a significant difference from that of WT at P<0.05 and <0.01, respectively, by Student's t-test.
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pone-0115128-g011: Relative expression level of IbMas and salt stress responsive genes in the IbMas-overexpressing sweetpotato plants.P5CS: pyrroline-5-carboxylate synthase; P5CR: pyrroline-5-carboxylate reductase; SOD: superoxide dismutase; psbA: encoding D1 protein; PRK: phosphoribulokinase (PRKase). The salt-tolerant transgenic plants (L52, L99, L102 and L101) and wild-type plant (WT) in vitro-grown plants were submerged for 12 h in 1/2 MS medium containing 0 and 200 mM NaCl, respectively. The sweetpotato β-actin gene was used as an internal control. The results are expressed as relative values based on WT grown under control condition as reference sample set to 1.0. Data are presented as means ± SE (n = 3). * and ** indicate a significant difference from that of WT at P<0.05 and <0.01, respectively, by Student's t-test.

Mentions: Expression of IbMas, proline biosynthesis, photosynthesis and SOD genes in the salt-tolerant transgenic plants was analyzed by qRT-PCR. The expression level of IbMas gene was significantly higher in the transgenic plants compared to wild-type plants (Fig. 11). To investigate the impact of IbMas overexpression on the transcription of salt stress response related genes, the expression of well-known salt stress responsive marker genes encoding pyrroline-5-carboxylate synthase (P5CS), pyrroline-5-carboxylate reductase (P5CR) and SOD was analyzed under salt stress (Fig. 11). P5CS, P5CR and SOD genes exhibited significantly increased expression level in the salt-tolerant transgenic plants compared to the wild-type under salt stress (Fig. 11). The expression level of psbA and PRK genes, which encode D1 protein and phosphoribulokinase (PRKase), respectively, was also higher in transgenic plants than in wild-type plants (Fig. 11).


A novel α/β-hydrolase gene IbMas enhances salt tolerance in transgenic sweetpotato.

Liu D, Wang L, Zhai H, Song X, He S, Liu Q - PLoS ONE (2014)

Relative expression level of IbMas and salt stress responsive genes in the IbMas-overexpressing sweetpotato plants.P5CS: pyrroline-5-carboxylate synthase; P5CR: pyrroline-5-carboxylate reductase; SOD: superoxide dismutase; psbA: encoding D1 protein; PRK: phosphoribulokinase (PRKase). The salt-tolerant transgenic plants (L52, L99, L102 and L101) and wild-type plant (WT) in vitro-grown plants were submerged for 12 h in 1/2 MS medium containing 0 and 200 mM NaCl, respectively. The sweetpotato β-actin gene was used as an internal control. The results are expressed as relative values based on WT grown under control condition as reference sample set to 1.0. Data are presented as means ± SE (n = 3). * and ** indicate a significant difference from that of WT at P<0.05 and <0.01, respectively, by Student's t-test.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4264881&req=5

pone-0115128-g011: Relative expression level of IbMas and salt stress responsive genes in the IbMas-overexpressing sweetpotato plants.P5CS: pyrroline-5-carboxylate synthase; P5CR: pyrroline-5-carboxylate reductase; SOD: superoxide dismutase; psbA: encoding D1 protein; PRK: phosphoribulokinase (PRKase). The salt-tolerant transgenic plants (L52, L99, L102 and L101) and wild-type plant (WT) in vitro-grown plants were submerged for 12 h in 1/2 MS medium containing 0 and 200 mM NaCl, respectively. The sweetpotato β-actin gene was used as an internal control. The results are expressed as relative values based on WT grown under control condition as reference sample set to 1.0. Data are presented as means ± SE (n = 3). * and ** indicate a significant difference from that of WT at P<0.05 and <0.01, respectively, by Student's t-test.
Mentions: Expression of IbMas, proline biosynthesis, photosynthesis and SOD genes in the salt-tolerant transgenic plants was analyzed by qRT-PCR. The expression level of IbMas gene was significantly higher in the transgenic plants compared to wild-type plants (Fig. 11). To investigate the impact of IbMas overexpression on the transcription of salt stress response related genes, the expression of well-known salt stress responsive marker genes encoding pyrroline-5-carboxylate synthase (P5CS), pyrroline-5-carboxylate reductase (P5CR) and SOD was analyzed under salt stress (Fig. 11). P5CS, P5CR and SOD genes exhibited significantly increased expression level in the salt-tolerant transgenic plants compared to the wild-type under salt stress (Fig. 11). The expression level of psbA and PRK genes, which encode D1 protein and phosphoribulokinase (PRKase), respectively, was also higher in transgenic plants than in wild-type plants (Fig. 11).

Bottom Line: Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type.Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants.H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing, China.

ABSTRACT
Salt stress is one of the major environmental stresses in agriculture worldwide and affects crop productivity and quality. The development of crops with elevated levels of salt tolerance is therefore highly desirable. In the present study, a novel maspardin gene, named IbMas, was isolated from salt-tolerant sweetpotato (Ipomoea batatas (L.) Lam.) line ND98. IbMas contains maspardin domain and belongs to α/β-hydrolase superfamily. Expression of IbMas was up-regulated in sweetpotato under salt stress and ABA treatment. The IbMas-overexpressing sweetpotato (cv. Shangshu 19) plants exhibited significantly higher salt tolerance compared with the wild-type. Proline content was significantly increased, whereas malonaldehyde content was significantly decreased in the transgenic plants. The activities of superoxide dismutase (SOD) and photosynthesis were significantly enhanced in the transgenic plants. H2O2 was also found to be significantly less accumulated in the transgenic plants than in the wild-type. Overexpression of IbMas up-regulated the salt stress responsive genes, including pyrroline-5-carboxylate synthase, pyrroline-5-carboxylate reductase, SOD, psbA and phosphoribulokinase genes, under salt stress. These findings suggest that overexpression of IbMas enhances salt tolerance of the transgenic sweetpotato plants by regulating osmotic balance, protecting membrane integrity and photosynthesis and increasing reactive oxygen species scavenging capacity.

No MeSH data available.


Related in: MedlinePlus