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Hippocampal spatial position evaluation on MRI for research and clinical practice.

Mrzílkova J, Koutela A, Kutová M, Patzelt M, Ibrahim I, Al-Kayssi D, Bartoš A, Řípová D, Čermáková P, Zach P - PLoS ONE (2014)

Bottom Line: We found that 1) hippocampal area of nat images is larger compared to hipp images, area of the nat images is equal to the AC-PC images and area of the hipp images is smaller compared to AC-PC images, 2) hippocampal area together with the area of the temporal horn for nat images is larger compared to hipp images, area of the hipp images is smaller compared to the AC-PC images and area of the nat images is smaller compared to the AC-PC images.The conclusion is that the measured area of the hippocampus in the native MRI is almost the same as the area of MRI reoriented only into the AC-PC axis.Surprising finding was that rotation of both AC-PC and hipp line towards x-axis among patients varies up to 35° and the same is true for the skull rotation so that it is not only a matter of the brain position.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.

ABSTRACT
In clinical practice as well as in many volumetric studies we use different reorientations of the brain position towards x and y axis on the magnetic resonance imaging (MRI) scans. In order to find out whether it has an overall effect on the resulting 2D data, manual hippocampal area measurements and rotation variability of the brain (in two reoriented axes) and the skull were performed in 23 Alzheimer's disease patients and 31 healthy controls. After the MRI scanning, native brain scans (nat) were reoriented into the two different artificial planes (anterior commissure-posterior commissure axis (AC-PC) and hippocampal horizontal long axis (hipp)). Hippocampal area and temporal horn of the lateral ventricle was measured manually using freeware Image J program. We found that 1) hippocampal area of nat images is larger compared to hipp images, area of the nat images is equal to the AC-PC images and area of the hipp images is smaller compared to AC-PC images, 2) hippocampal area together with the area of the temporal horn for nat images is larger compared to hipp images, area of the hipp images is smaller compared to the AC-PC images and area of the nat images is smaller compared to the AC-PC images. The conclusion is that the measured area of the hippocampus in the native MRI is almost the same as the area of MRI reoriented only into the AC-PC axis. Therefore, when performing 2D area studies of the hippocampus or in the clinical practice we recommend usage of not-reoriented MRI images or to reorient them into the AC-PC axis. Surprising finding was that rotation of both AC-PC and hipp line towards x-axis among patients varies up to 35° and the same is true for the skull rotation so that it is not only a matter of the brain position.

No MeSH data available.


Related in: MedlinePlus

Example of manual anatomical delineation of the hippocampus (white line with grey area inside) and temporal horn of the lateral ventricle (white line with black area inside) on the left and right side.Coronal sections of the MRI are located at the transition from the alveus of the hippocampus into the hippocampal body.
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pone-0115174-g002: Example of manual anatomical delineation of the hippocampus (white line with grey area inside) and temporal horn of the lateral ventricle (white line with black area inside) on the left and right side.Coronal sections of the MRI are located at the transition from the alveus of the hippocampus into the hippocampal body.

Mentions: We measured only the area of the hippocampus proper – without subiculum and parahippocampal gyrus. However, these measurements did include dentate gyrus due to its close position to the hippocampus on the frontal section (Fig. 2). This method is in line with a large amount of studies that use a similar protocol for hipppocampal delineation, for example [17]. The inferior border of the measured area was a clearly visible line between hippocampus and the grey matter of parahippocampal gyrus medially and directly caudally white matter of the subiculum. The caudo-medial border was set as cerebrospinal fluid (CSF) filled space between hippocampus and crura cerebri on both sides and cranio-medial border as the point where fornix (included in the measurement) inserts on the roof of the temporal horn of the lateral ventricle via stria medullaris. The lateral border of the measured area was a clearly visible round shape of the hippocampus with dark black color of the cerebrospinal fluid in the temporal horn of the lateral ventricle.


Hippocampal spatial position evaluation on MRI for research and clinical practice.

Mrzílkova J, Koutela A, Kutová M, Patzelt M, Ibrahim I, Al-Kayssi D, Bartoš A, Řípová D, Čermáková P, Zach P - PLoS ONE (2014)

Example of manual anatomical delineation of the hippocampus (white line with grey area inside) and temporal horn of the lateral ventricle (white line with black area inside) on the left and right side.Coronal sections of the MRI are located at the transition from the alveus of the hippocampus into the hippocampal body.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264873&req=5

pone-0115174-g002: Example of manual anatomical delineation of the hippocampus (white line with grey area inside) and temporal horn of the lateral ventricle (white line with black area inside) on the left and right side.Coronal sections of the MRI are located at the transition from the alveus of the hippocampus into the hippocampal body.
Mentions: We measured only the area of the hippocampus proper – without subiculum and parahippocampal gyrus. However, these measurements did include dentate gyrus due to its close position to the hippocampus on the frontal section (Fig. 2). This method is in line with a large amount of studies that use a similar protocol for hipppocampal delineation, for example [17]. The inferior border of the measured area was a clearly visible line between hippocampus and the grey matter of parahippocampal gyrus medially and directly caudally white matter of the subiculum. The caudo-medial border was set as cerebrospinal fluid (CSF) filled space between hippocampus and crura cerebri on both sides and cranio-medial border as the point where fornix (included in the measurement) inserts on the roof of the temporal horn of the lateral ventricle via stria medullaris. The lateral border of the measured area was a clearly visible round shape of the hippocampus with dark black color of the cerebrospinal fluid in the temporal horn of the lateral ventricle.

Bottom Line: We found that 1) hippocampal area of nat images is larger compared to hipp images, area of the nat images is equal to the AC-PC images and area of the hipp images is smaller compared to AC-PC images, 2) hippocampal area together with the area of the temporal horn for nat images is larger compared to hipp images, area of the hipp images is smaller compared to the AC-PC images and area of the nat images is smaller compared to the AC-PC images.The conclusion is that the measured area of the hippocampus in the native MRI is almost the same as the area of MRI reoriented only into the AC-PC axis.Surprising finding was that rotation of both AC-PC and hipp line towards x-axis among patients varies up to 35° and the same is true for the skull rotation so that it is not only a matter of the brain position.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy, Third Faculty of Medicine, Charles University, Ruská 87, 100 00 Prague 10, Czech Republic.

ABSTRACT
In clinical practice as well as in many volumetric studies we use different reorientations of the brain position towards x and y axis on the magnetic resonance imaging (MRI) scans. In order to find out whether it has an overall effect on the resulting 2D data, manual hippocampal area measurements and rotation variability of the brain (in two reoriented axes) and the skull were performed in 23 Alzheimer's disease patients and 31 healthy controls. After the MRI scanning, native brain scans (nat) were reoriented into the two different artificial planes (anterior commissure-posterior commissure axis (AC-PC) and hippocampal horizontal long axis (hipp)). Hippocampal area and temporal horn of the lateral ventricle was measured manually using freeware Image J program. We found that 1) hippocampal area of nat images is larger compared to hipp images, area of the nat images is equal to the AC-PC images and area of the hipp images is smaller compared to AC-PC images, 2) hippocampal area together with the area of the temporal horn for nat images is larger compared to hipp images, area of the hipp images is smaller compared to the AC-PC images and area of the nat images is smaller compared to the AC-PC images. The conclusion is that the measured area of the hippocampus in the native MRI is almost the same as the area of MRI reoriented only into the AC-PC axis. Therefore, when performing 2D area studies of the hippocampus or in the clinical practice we recommend usage of not-reoriented MRI images or to reorient them into the AC-PC axis. Surprising finding was that rotation of both AC-PC and hipp line towards x-axis among patients varies up to 35° and the same is true for the skull rotation so that it is not only a matter of the brain position.

No MeSH data available.


Related in: MedlinePlus