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Biocompatible flavone-based fluorogenic probes for quick wash-free mitochondrial imaging in living cells.

Liu B, Shah M, Zhang G, Liu Q, Pang Y - ACS Appl Mater Interfaces (2014)

Bottom Line: Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms.The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining.The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Maurice Morton Institute of Polymer Science, ‡Department of Biomedical Engineering, and §Department of Biology, The University of Akron , Akron, Ohio 44325, United States.

ABSTRACT
Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.

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Normalized UV spectraand FL spectra of MC-Mito1 (dashed lines) and MC-Mito2 (solid lines) in a DMSO solution.
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fig3: Normalized UV spectraand FL spectra of MC-Mito1 (dashed lines) and MC-Mito2 (solid lines) in a DMSO solution.

Mentions: In a DMSO solution, MC-Mito1 and MC-Mito2 exhibited similar photophysical properties(Figure 3), showing absorption maxima at 373nm and emission maxima at 500 nm. The emission spectra of dyes werewell separated from the absorption, revealing a large Stokes shift(∼127 nm) that is highly desirable for fluorescence imaging.The environmentally sensitive properties of MC-Mito1 and MC-Mito2 were examined in different solvents (Figure S9 ofthe Supporting Information). The resultsindicate that MC-Mito1 and MC-Mito2 showedclassical positive solvatochromism features of flavone dyes,44 with a correlation between the emission maximumand relative solvent polarity. The emission spectra shifted dramaticallyto longer wavelengths (from 450 to 540 nm) as the solvent polaritywas increased. The probe’s ability to shift the fluorescencefrom a polar to nonpolar environment could also facilitate the wash-freeapplication, as the cell-bound dyes gave emission at wavelengths distinctfrom those of the free dye in an aqueous solution.


Biocompatible flavone-based fluorogenic probes for quick wash-free mitochondrial imaging in living cells.

Liu B, Shah M, Zhang G, Liu Q, Pang Y - ACS Appl Mater Interfaces (2014)

Normalized UV spectraand FL spectra of MC-Mito1 (dashed lines) and MC-Mito2 (solid lines) in a DMSO solution.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264855&req=5

fig3: Normalized UV spectraand FL spectra of MC-Mito1 (dashed lines) and MC-Mito2 (solid lines) in a DMSO solution.
Mentions: In a DMSO solution, MC-Mito1 and MC-Mito2 exhibited similar photophysical properties(Figure 3), showing absorption maxima at 373nm and emission maxima at 500 nm. The emission spectra of dyes werewell separated from the absorption, revealing a large Stokes shift(∼127 nm) that is highly desirable for fluorescence imaging.The environmentally sensitive properties of MC-Mito1 and MC-Mito2 were examined in different solvents (Figure S9 ofthe Supporting Information). The resultsindicate that MC-Mito1 and MC-Mito2 showedclassical positive solvatochromism features of flavone dyes,44 with a correlation between the emission maximumand relative solvent polarity. The emission spectra shifted dramaticallyto longer wavelengths (from 450 to 540 nm) as the solvent polaritywas increased. The probe’s ability to shift the fluorescencefrom a polar to nonpolar environment could also facilitate the wash-freeapplication, as the cell-bound dyes gave emission at wavelengths distinctfrom those of the free dye in an aqueous solution.

Bottom Line: Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms.The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining.The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Maurice Morton Institute of Polymer Science, ‡Department of Biomedical Engineering, and §Department of Biology, The University of Akron , Akron, Ohio 44325, United States.

ABSTRACT
Mitochondria, vital organelles existing in almost all eukaryotic cells, play a crucial role in energy metabolism and apoptosis of aerobic organisms. In this work, we report two new flavone-based fluorescent probes, MC-Mito1 and MC-Mito2, for monitoring mitochondria in living cells. These two probes exhibit remarkably low toxicity, good cell permeability, and high specificity; these probes complement the existing library of mitochondrial imaging agents. The new dyes give nearly no background fluorescence, and their application does not require tedious postwashing after cell staining. The appreciable tolerance of MC-Mito2 encourages a broader range of biological applications for understanding the cell degeneration and apoptosis mechanism.

Show MeSH
Related in: MedlinePlus