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D-Glucose-Induced Cytotoxic, Genotoxic, and Apoptotic Effects on Human Breast Adenocarcinoma (MCF-7) Cells.

Tchounwou CK, Yedjou CG, Farah I, Tchounwou PB - J Cancer Sci Ther (2014)

Bottom Line: The results of MTT assay indicated that D-glucose significantly reduces the viability of MCF-7 cells in a dose and time-dependent manner.Taking together, these data provide clear evidence that D-glucose induces cytotoxic, genotoxic, and apoptotic effects on MCF-7 cells.This finding represents the basis for further studies addressing the pathophysiological mechanisms of action of glucose overdose.

View Article: PubMed Central - PubMed

Affiliation: Cellomics and Toxicogenomics Research Laboratory, NIH-Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, USA.

ABSTRACT

Introduction: Glucose is a simple sugar that plays an important role in energy production in biological systems. However, it has been linked to many long-term health problems including the risk of heart disease and stroke, erectile dysfunction in men and pregnancy complications in women, and damage to the kidneys, nerves, eye and vision. Also, the underlying mechanisms of diabetic complications are poorly understood.

Methods: In the present study, D-glucose-induced cytotoxic, genotoxic, and apoptotic effects were studied using MCF-7 cells as an in vitro test model. Cell viability was determined by MTT assay. Genotoxic damage was tested by the means of alkaline single cell gel electrophoresis (Comet) assay. Cell apoptosis was measured by flow cytometry assessment (Annexin-V/PI assay).

Results: The results of MTT assay indicated that D-glucose significantly reduces the viability of MCF-7 cells in a dose and time-dependent manner. Similar trend was obtained with the trypan blue exclusion test. Data obtained from the Comet assay indicated that D-glucose causes DNA damage in MCF-7 cells in a dose-dependent manner. The flow cytometry assessment (Annexin V FITC/PI) showed a strong dose-response relationship between D-glucose exposure and annexin V positive MCF-7 cells undergoing early apoptosis.

Conclusion: Taking together, these data provide clear evidence that D-glucose induces cytotoxic, genotoxic, and apoptotic effects on MCF-7 cells. This finding represents the basis for further studies addressing the pathophysiological mechanisms of action of glucose overdose.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic effects of D-glucose to MCF-7 cells. Cells were cultured with different doses of D-glucose for 1 and 2 h as indicated in the Materials and Methods. Cell viability was determined based on the MTT assay. *Significantly different (p < 0.05) from the control, according to the Dunnett’s test.
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Figure 1: Cytotoxic effects of D-glucose to MCF-7 cells. Cells were cultured with different doses of D-glucose for 1 and 2 h as indicated in the Materials and Methods. Cell viability was determined based on the MTT assay. *Significantly different (p < 0.05) from the control, according to the Dunnett’s test.

Mentions: Cells were treated with increasing concentrations of D-glucose for 1 h and 2 h, and viable cells were monitored by MTT [3–(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay. Treatment of MCF-7 cells with 5, 10, 20, 40 and 80 mg/mL VA for 1 h decreased cell viability to approximately 4%, 0%, 25%, 30% and 40% respectively (Figure 1). Exposing MCF-7 cells for 2 h increased viable cell counts by 6% in 5 mg/mL D-glucose and decreased cell viability to approximately 18%, 20%, 49% and 52% in 10, 20, 40 and 80 mg/ mL D-glucose, respectively (Figure 1). Overall, data obtained from the MTT assay demonstrated that D-glucose treatment reduced cell viability in a concentration and time-dependent manner.


D-Glucose-Induced Cytotoxic, Genotoxic, and Apoptotic Effects on Human Breast Adenocarcinoma (MCF-7) Cells.

Tchounwou CK, Yedjou CG, Farah I, Tchounwou PB - J Cancer Sci Ther (2014)

Cytotoxic effects of D-glucose to MCF-7 cells. Cells were cultured with different doses of D-glucose for 1 and 2 h as indicated in the Materials and Methods. Cell viability was determined based on the MTT assay. *Significantly different (p < 0.05) from the control, according to the Dunnett’s test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264661&req=5

Figure 1: Cytotoxic effects of D-glucose to MCF-7 cells. Cells were cultured with different doses of D-glucose for 1 and 2 h as indicated in the Materials and Methods. Cell viability was determined based on the MTT assay. *Significantly different (p < 0.05) from the control, according to the Dunnett’s test.
Mentions: Cells were treated with increasing concentrations of D-glucose for 1 h and 2 h, and viable cells were monitored by MTT [3–(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay. Treatment of MCF-7 cells with 5, 10, 20, 40 and 80 mg/mL VA for 1 h decreased cell viability to approximately 4%, 0%, 25%, 30% and 40% respectively (Figure 1). Exposing MCF-7 cells for 2 h increased viable cell counts by 6% in 5 mg/mL D-glucose and decreased cell viability to approximately 18%, 20%, 49% and 52% in 10, 20, 40 and 80 mg/ mL D-glucose, respectively (Figure 1). Overall, data obtained from the MTT assay demonstrated that D-glucose treatment reduced cell viability in a concentration and time-dependent manner.

Bottom Line: The results of MTT assay indicated that D-glucose significantly reduces the viability of MCF-7 cells in a dose and time-dependent manner.Taking together, these data provide clear evidence that D-glucose induces cytotoxic, genotoxic, and apoptotic effects on MCF-7 cells.This finding represents the basis for further studies addressing the pathophysiological mechanisms of action of glucose overdose.

View Article: PubMed Central - PubMed

Affiliation: Cellomics and Toxicogenomics Research Laboratory, NIH-Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, USA.

ABSTRACT

Introduction: Glucose is a simple sugar that plays an important role in energy production in biological systems. However, it has been linked to many long-term health problems including the risk of heart disease and stroke, erectile dysfunction in men and pregnancy complications in women, and damage to the kidneys, nerves, eye and vision. Also, the underlying mechanisms of diabetic complications are poorly understood.

Methods: In the present study, D-glucose-induced cytotoxic, genotoxic, and apoptotic effects were studied using MCF-7 cells as an in vitro test model. Cell viability was determined by MTT assay. Genotoxic damage was tested by the means of alkaline single cell gel electrophoresis (Comet) assay. Cell apoptosis was measured by flow cytometry assessment (Annexin-V/PI assay).

Results: The results of MTT assay indicated that D-glucose significantly reduces the viability of MCF-7 cells in a dose and time-dependent manner. Similar trend was obtained with the trypan blue exclusion test. Data obtained from the Comet assay indicated that D-glucose causes DNA damage in MCF-7 cells in a dose-dependent manner. The flow cytometry assessment (Annexin V FITC/PI) showed a strong dose-response relationship between D-glucose exposure and annexin V positive MCF-7 cells undergoing early apoptosis.

Conclusion: Taking together, these data provide clear evidence that D-glucose induces cytotoxic, genotoxic, and apoptotic effects on MCF-7 cells. This finding represents the basis for further studies addressing the pathophysiological mechanisms of action of glucose overdose.

No MeSH data available.


Related in: MedlinePlus