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Reduction of a multidrug-resistant pathogen and associated virulence factors in a burn wound infection model: further understanding of the effectiveness of a hydroconductive dressing.

Carney BC, Ortiz RT, Bullock RM, Prindeze NJ, Moffatt LT, Robson MC, Shupp JW - Eplasty (2014)

Bottom Line: Drawtex's ability to remove pathogens and associated virulence factors has been demonstrated in vitro.Quantities remained lower in the experimental group through day 14 (P < .001).The hydroconductive dressing provided a significant reduction in pathogen and virulence factors compared to a control dressing.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Objective: Drawtex's ability to remove pathogens and associated virulence factors has been demonstrated in vitro. A model of burn wound infection was used to characterize the in vivo impact of this dressing on infection and wound healing.

Methods: Paired burn wounds were created on the dorsum of Sprague Dawley rats and were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Animals were divided into 2 groups, half with wounds that received experimental dressing and the remaining half with control dressing-treated wounds. Dressings remained in place through 3, 6, 9, or 14 days after injury, and methicillin-resistant S aureus and virulence factors were quantified. Laser Doppler imaging was used to examine wound perfusion, and local host immune response was assessed through the quantification of mRNA expression.

Results: By day 3, less methicillin-resistant S aureus was measured in wounds treated with experimental-dressing compared to control-dressing wounds. Quantities remained lower in the experimental group through day 14 (P < .001). More methicillin-resistant S aureus was quantified in the experimental dressing itself than in control dressing at all time points (P < .05). Experimental dressing-treated wounds contained less toxic shock syndrome toxin 1 and Panton-Valentine leukocidin than controls (P < .01) on days 6, 9, and 14. Induction of toll-like receptor 2, NOD-like receptor family, pyrin domain containing 3, and interleukin 6 was significantly lower in experimental-dressing treated wounds than in controls on days 6 and 9 (P < .05).

Conclusions: The hydroconductive dressing provided a significant reduction in pathogen and virulence factors compared to a control dressing. As a result of clearance of virulence factors from the wound bed, a requisite alteration in host innate immune response was observed.

No MeSH data available.


Related in: MedlinePlus

Fold change in mRNA expression of innate immunity-related genes from levels quantified in preinjury, day 0 skin samples. Comparisons between experimental and control dressing-treated wounds in the induction of TLR2 (A), NLRP3 (B), and IL6 (C) at each time point were made using multiple t tests with a Holm-Sidak correction for multiple tests (P < .05), with statistically significant differences indicated (*). Data are shown as means with standard deviations. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; IL6, interleukin 6; NLRP3, NOD-like receptor family, pyrin domain containing 3; TLR2, toll-like receptor 2.
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Figure 5: Fold change in mRNA expression of innate immunity-related genes from levels quantified in preinjury, day 0 skin samples. Comparisons between experimental and control dressing-treated wounds in the induction of TLR2 (A), NLRP3 (B), and IL6 (C) at each time point were made using multiple t tests with a Holm-Sidak correction for multiple tests (P < .05), with statistically significant differences indicated (*). Data are shown as means with standard deviations. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; IL6, interleukin 6; NLRP3, NOD-like receptor family, pyrin domain containing 3; TLR2, toll-like receptor 2.

Mentions: Induction of TLR2, NLRP3, and IL6 was significantly lower in experimental dressing-treated wounds than in controls on days 6 and 9 (P < .05; Fig 5). Induction of TLR2, NLRP3, and IL6 appeared to be higher in control dressing-treated wounds than in experimentally treated wounds by day 14, though these differences were not statistically significant (Fig 3).


Reduction of a multidrug-resistant pathogen and associated virulence factors in a burn wound infection model: further understanding of the effectiveness of a hydroconductive dressing.

Carney BC, Ortiz RT, Bullock RM, Prindeze NJ, Moffatt LT, Robson MC, Shupp JW - Eplasty (2014)

Fold change in mRNA expression of innate immunity-related genes from levels quantified in preinjury, day 0 skin samples. Comparisons between experimental and control dressing-treated wounds in the induction of TLR2 (A), NLRP3 (B), and IL6 (C) at each time point were made using multiple t tests with a Holm-Sidak correction for multiple tests (P < .05), with statistically significant differences indicated (*). Data are shown as means with standard deviations. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; IL6, interleukin 6; NLRP3, NOD-like receptor family, pyrin domain containing 3; TLR2, toll-like receptor 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264520&req=5

Figure 5: Fold change in mRNA expression of innate immunity-related genes from levels quantified in preinjury, day 0 skin samples. Comparisons between experimental and control dressing-treated wounds in the induction of TLR2 (A), NLRP3 (B), and IL6 (C) at each time point were made using multiple t tests with a Holm-Sidak correction for multiple tests (P < .05), with statistically significant differences indicated (*). Data are shown as means with standard deviations. GAPDH indicates glyceraldehyde 3-phosphate dehydrogenase; IL6, interleukin 6; NLRP3, NOD-like receptor family, pyrin domain containing 3; TLR2, toll-like receptor 2.
Mentions: Induction of TLR2, NLRP3, and IL6 was significantly lower in experimental dressing-treated wounds than in controls on days 6 and 9 (P < .05; Fig 5). Induction of TLR2, NLRP3, and IL6 appeared to be higher in control dressing-treated wounds than in experimentally treated wounds by day 14, though these differences were not statistically significant (Fig 3).

Bottom Line: Drawtex's ability to remove pathogens and associated virulence factors has been demonstrated in vitro.Quantities remained lower in the experimental group through day 14 (P < .001).The hydroconductive dressing provided a significant reduction in pathogen and virulence factors compared to a control dressing.

View Article: PubMed Central - PubMed

Affiliation: Firefighters' Burn and Surgical Research Laboratory, MedStar Health Research Institute, Washington, DC.

ABSTRACT

Objective: Drawtex's ability to remove pathogens and associated virulence factors has been demonstrated in vitro. A model of burn wound infection was used to characterize the in vivo impact of this dressing on infection and wound healing.

Methods: Paired burn wounds were created on the dorsum of Sprague Dawley rats and were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Animals were divided into 2 groups, half with wounds that received experimental dressing and the remaining half with control dressing-treated wounds. Dressings remained in place through 3, 6, 9, or 14 days after injury, and methicillin-resistant S aureus and virulence factors were quantified. Laser Doppler imaging was used to examine wound perfusion, and local host immune response was assessed through the quantification of mRNA expression.

Results: By day 3, less methicillin-resistant S aureus was measured in wounds treated with experimental-dressing compared to control-dressing wounds. Quantities remained lower in the experimental group through day 14 (P < .001). More methicillin-resistant S aureus was quantified in the experimental dressing itself than in control dressing at all time points (P < .05). Experimental dressing-treated wounds contained less toxic shock syndrome toxin 1 and Panton-Valentine leukocidin than controls (P < .01) on days 6, 9, and 14. Induction of toll-like receptor 2, NOD-like receptor family, pyrin domain containing 3, and interleukin 6 was significantly lower in experimental-dressing treated wounds than in controls on days 6 and 9 (P < .05).

Conclusions: The hydroconductive dressing provided a significant reduction in pathogen and virulence factors compared to a control dressing. As a result of clearance of virulence factors from the wound bed, a requisite alteration in host innate immune response was observed.

No MeSH data available.


Related in: MedlinePlus