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H-NS is a repressor of major virulence gene loci in Vibrio parahaemolyticus.

Sun F, Zhang Y, Qiu Y, Yang H, Yang W, Yin Z, Wang J, Yang R, Xia P, Zhou D - Front Microbiol (2014)

Bottom Line: Vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci T3SS1, Vp-PAI (T3SS1+tdh2) and T6SS2.H-NS appears to act as a major repressor of the virulence of this pathogen.Date presented here would promote us to gain a deeper understanding of H-NS-mediated silencing of horizontally acquired virulence loci in V. parahaemolyticus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University Chongqing, China.

ABSTRACT
Vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci T3SS1, Vp-PAI (T3SS1+tdh2) and T6SS2. As showing in this study, the nucleoid-associated DNA-binding regulator H-NS binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover H-NS inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of V. parahaemolyticus. H-NS appears to act as a major repressor of the virulence of this pathogen. Date presented here would promote us to gain a deeper understanding of H-NS-mediated silencing of horizontally acquired virulence loci in V. parahaemolyticus.

No MeSH data available.


Related in: MedlinePlus

Regulation of Vp-PAI genes by H-NS. qRT-PCR (A), primer extension (B), LacZ fusion (C), EMSA (D), and DNase I footprinting (E) were performed as Figure 2.
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Figure 3: Regulation of Vp-PAI genes by H-NS. qRT-PCR (A), primer extension (B), LacZ fusion (C), EMSA (D), and DNase I footprinting (E) were performed as Figure 2.

Mentions: The mRNA levels of each target gene in Δhns and WT were determined by qRT-PCR (Figures 2A, 3A, 4A) and primer extension (Figures 2B, 3B, 4B). The results from both kinds of assay showed that the mRNA level of each target was significantly enhanced in Δhns relative to WT. To test action of H-NS on the promoter activity of each target gene, the fusion promoter consisting of a promoter-proximal region of each target gene together with the promoterless lacZ was constructed in pHRP309, and then transformed into WT and Δhns, respectively (Figures 2C, 3C, 4C); the relative promoter activity (the β-Galactosidase activity) of each target gene in Δhns was significantly higher than that in Δhns. The above results disclosed the negative regulation of each target gene by H-NS.


H-NS is a repressor of major virulence gene loci in Vibrio parahaemolyticus.

Sun F, Zhang Y, Qiu Y, Yang H, Yang W, Yin Z, Wang J, Yang R, Xia P, Zhou D - Front Microbiol (2014)

Regulation of Vp-PAI genes by H-NS. qRT-PCR (A), primer extension (B), LacZ fusion (C), EMSA (D), and DNase I footprinting (E) were performed as Figure 2.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264476&req=5

Figure 3: Regulation of Vp-PAI genes by H-NS. qRT-PCR (A), primer extension (B), LacZ fusion (C), EMSA (D), and DNase I footprinting (E) were performed as Figure 2.
Mentions: The mRNA levels of each target gene in Δhns and WT were determined by qRT-PCR (Figures 2A, 3A, 4A) and primer extension (Figures 2B, 3B, 4B). The results from both kinds of assay showed that the mRNA level of each target was significantly enhanced in Δhns relative to WT. To test action of H-NS on the promoter activity of each target gene, the fusion promoter consisting of a promoter-proximal region of each target gene together with the promoterless lacZ was constructed in pHRP309, and then transformed into WT and Δhns, respectively (Figures 2C, 3C, 4C); the relative promoter activity (the β-Galactosidase activity) of each target gene in Δhns was significantly higher than that in Δhns. The above results disclosed the negative regulation of each target gene by H-NS.

Bottom Line: Vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci T3SS1, Vp-PAI (T3SS1+tdh2) and T6SS2.H-NS appears to act as a major repressor of the virulence of this pathogen.Date presented here would promote us to gain a deeper understanding of H-NS-mediated silencing of horizontally acquired virulence loci in V. parahaemolyticus.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Southwest Hospital, Third Military Medical University Chongqing, China.

ABSTRACT
Vibrio parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis, harbors three major virulence gene loci T3SS1, Vp-PAI (T3SS1+tdh2) and T6SS2. As showing in this study, the nucleoid-associated DNA-binding regulator H-NS binds to multiple promoter-proximal regions in each of the above three loci to repress their transcription, and moreover H-NS inhibits the cytotoxicitiy, enterotoxicity, hemolytic activity, and mouse lethality of V. parahaemolyticus. H-NS appears to act as a major repressor of the virulence of this pathogen. Date presented here would promote us to gain a deeper understanding of H-NS-mediated silencing of horizontally acquired virulence loci in V. parahaemolyticus.

No MeSH data available.


Related in: MedlinePlus