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HmsB enhances biofilm formation in Yersinia pestis.

Fang N, Qu S, Yang H, Fang H, Liu L, Zhang Y, Wang L, Han Y, Zhou D, Yang R - Front Microbiol (2014)

Bottom Line: The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide.Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP.HmsB most likely acts as a major activator of biofilm formation in Y. pestis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.

ABSTRACT
The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide. Yersinia pestis expresses the two sole diguanylate cyclases HmsT and HmsD and the sole phosphodiesterase HmsP, which are specific for biosynthesis and degradation, respectively, of 3',5'-cyclic diguanosine monophosphate (c-di-GMP), a second messenger promoting exopolysaccharide production. In this work, the phenotypic assays indicates that Y. pestis sRNA HmsB enhances the production of c-di-GMP, exopolysaccharide, and biofilm. Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP. HmsB most likely acts as a major activator of biofilm formation in Y. pestis. This is the first report of regulation of Yersinia biofilm formation by a sRNA. Data presented here will promote us to gain a deeper understanding of the complex regulatory circuits controlling Yersinia biofilm formation.

No MeSH data available.


Related in: MedlinePlus

Genetic organization of hmsB and hmsCDE. Boxed arrows represented length and direction of indicated ORFs. Broken arrows indicated transcription starts (i.e., transcribed promoters). Please refer to our companion submission for regulatory action of RcsAB on hmsB and hmsCDE.
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Figure 1: Genetic organization of hmsB and hmsCDE. Boxed arrows represented length and direction of indicated ORFs. Broken arrows indicated transcription starts (i.e., transcribed promoters). Please refer to our companion submission for regulatory action of RcsAB on hmsB and hmsCDE.

Mentions: Our previous RNA-seq study of Y. pestis (Yan et al., 2013) identified a temperature-dependent sRNA named sRNA035 located nearby hmsCDE (Figure 1), which promoted us to test whether sRNA035 was involved in regulating the production of c-di-GMP and biofilm formation. This sRNA was designated HmsB herein. This follow-up study disclosed that HmsB positively regulated hmsCDE, hmsT, hmsHFRS and its own gene but negatively regulated hmsP, and thus acted as a major activator of c-di-GMP, exopolysaccharide and biofilm production in Y. pestis.


HmsB enhances biofilm formation in Yersinia pestis.

Fang N, Qu S, Yang H, Fang H, Liu L, Zhang Y, Wang L, Han Y, Zhou D, Yang R - Front Microbiol (2014)

Genetic organization of hmsB and hmsCDE. Boxed arrows represented length and direction of indicated ORFs. Broken arrows indicated transcription starts (i.e., transcribed promoters). Please refer to our companion submission for regulatory action of RcsAB on hmsB and hmsCDE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264472&req=5

Figure 1: Genetic organization of hmsB and hmsCDE. Boxed arrows represented length and direction of indicated ORFs. Broken arrows indicated transcription starts (i.e., transcribed promoters). Please refer to our companion submission for regulatory action of RcsAB on hmsB and hmsCDE.
Mentions: Our previous RNA-seq study of Y. pestis (Yan et al., 2013) identified a temperature-dependent sRNA named sRNA035 located nearby hmsCDE (Figure 1), which promoted us to test whether sRNA035 was involved in regulating the production of c-di-GMP and biofilm formation. This sRNA was designated HmsB herein. This follow-up study disclosed that HmsB positively regulated hmsCDE, hmsT, hmsHFRS and its own gene but negatively regulated hmsP, and thus acted as a major activator of c-di-GMP, exopolysaccharide and biofilm production in Y. pestis.

Bottom Line: The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide.Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP.HmsB most likely acts as a major activator of biofilm formation in Y. pestis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology Beijing, China.

ABSTRACT
The hmsHFRS operon is responsible for biosynthesis and translocation of biofilm matrix exopolysaccharide. Yersinia pestis expresses the two sole diguanylate cyclases HmsT and HmsD and the sole phosphodiesterase HmsP, which are specific for biosynthesis and degradation, respectively, of 3',5'-cyclic diguanosine monophosphate (c-di-GMP), a second messenger promoting exopolysaccharide production. In this work, the phenotypic assays indicates that Y. pestis sRNA HmsB enhances the production of c-di-GMP, exopolysaccharide, and biofilm. Further gene regulation experiments disclose that HmsB stimulates the expression of hmsB, hmsCDE, hmsT, and hmsHFRS but represses that of hmsP. HmsB most likely acts as a major activator of biofilm formation in Y. pestis. This is the first report of regulation of Yersinia biofilm formation by a sRNA. Data presented here will promote us to gain a deeper understanding of the complex regulatory circuits controlling Yersinia biofilm formation.

No MeSH data available.


Related in: MedlinePlus