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Field and controlled environment measurements show strong seasonal acclimation in photosynthesis and respiration potential in boreal Scots pine.

Kolari P, Chan T, Porcar-Castell A, Bäck J, Nikinmaa E, Juurola E - Front Plant Sci (2014)

Bottom Line: Despite the downregulation, the photosynthetic machinery retained a significant capacity during winter, which was not visible in the field measurements.On the other hand, the operating quantum yield of photosystem II and the initial slope of photosynthetic light response stayed almost at the summertime level until late autumn while at the same time P sat decreased following the prevailing temperature.Comparison of photosynthetic parameters with the environmental drivers suggests that light and minimum temperature are also decisive factors in the seasonal acclimation of photosynthesis in boreal evergreen trees.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Helsinki Helsinki, Finland.

ABSTRACT
Understanding the seasonality of photosynthesis in boreal evergreen trees and its control by the environment requires separation of the instantaneous and slow responses, as well as the dynamics of light reactions, carbon reactions, and respiration. We determined the seasonality of photosynthetic light response and respiration parameters of Scots pine (Pinus sylvestris L.) in the field in southern Finland and in controlled laboratory conditions. CO2 exchange and chlorophyll fluorescence were measured in the field using a continuously operated automated chamber setup and fluorescence monitoring systems. We also carried out monthly measurements of photosynthetic light, CO2 and temperature responses in standard conditions with a portable IRGA and fluorometer instrument. The field and response measurements indicated strong seasonal variability in the state of the photosynthetic machinery with a deep downregulation during winter. Despite the downregulation, the photosynthetic machinery retained a significant capacity during winter, which was not visible in the field measurements. Light-saturated photosynthesis (P sat) and the initial slope of the photosynthetic light response (α) obtained in standard conditions were up to 20% of their respective summertime values. Respiration also showed seasonal acclimation with peak values of respiration in standard temperature in spring and decline in autumn. Spring recovery of all photosynthetic parameters could be predicted with temperature history. On the other hand, the operating quantum yield of photosystem II and the initial slope of photosynthetic light response stayed almost at the summertime level until late autumn while at the same time P sat decreased following the prevailing temperature. Comparison of photosynthetic parameters with the environmental drivers suggests that light and minimum temperature are also decisive factors in the seasonal acclimation of photosynthesis in boreal evergreen trees.

No MeSH data available.


Related in: MedlinePlus

Temperature response of P1200 and αs determined from the light response measurements in controlled conditions. Each symbol corresponds to the monthly value of P1200 or αs determined in prevailing ambient temperature vs. corresponding parameter in the standard temperature (18°C). The lines denote the photosynthetic temperature response model (Equation 7) fitted to P1200 (P(T), solid line) and αs (α(T), dash line). The parameters defining the the response at sub-zero temperatures were fixed to values that make the curve reach zero at −5°C based on the continuous chamber data from several years.
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Figure 6: Temperature response of P1200 and αs determined from the light response measurements in controlled conditions. Each symbol corresponds to the monthly value of P1200 or αs determined in prevailing ambient temperature vs. corresponding parameter in the standard temperature (18°C). The lines denote the photosynthetic temperature response model (Equation 7) fitted to P1200 (P(T), solid line) and αs (α(T), dash line). The parameters defining the the response at sub-zero temperatures were fixed to values that make the curve reach zero at −5°C based on the continuous chamber data from several years.

Mentions: The initial slope of the light response (αs) and P1200 in the monthly response measurements showed similar relationships between the prevailing ambient temperature and reference temperature (Figure 6). Relative P1200 had slightly steeper slope of the temperature response than αs. Temperatures below zero were not used in the response measurements but the automatic chamber data indicates steep drop in photosynthetic rate below 0°C and the CO2 exchange signal, including respiration, diminishes at about −5°C (data not shown, see model approximation in Figure 6).


Field and controlled environment measurements show strong seasonal acclimation in photosynthesis and respiration potential in boreal Scots pine.

Kolari P, Chan T, Porcar-Castell A, Bäck J, Nikinmaa E, Juurola E - Front Plant Sci (2014)

Temperature response of P1200 and αs determined from the light response measurements in controlled conditions. Each symbol corresponds to the monthly value of P1200 or αs determined in prevailing ambient temperature vs. corresponding parameter in the standard temperature (18°C). The lines denote the photosynthetic temperature response model (Equation 7) fitted to P1200 (P(T), solid line) and αs (α(T), dash line). The parameters defining the the response at sub-zero temperatures were fixed to values that make the curve reach zero at −5°C based on the continuous chamber data from several years.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264410&req=5

Figure 6: Temperature response of P1200 and αs determined from the light response measurements in controlled conditions. Each symbol corresponds to the monthly value of P1200 or αs determined in prevailing ambient temperature vs. corresponding parameter in the standard temperature (18°C). The lines denote the photosynthetic temperature response model (Equation 7) fitted to P1200 (P(T), solid line) and αs (α(T), dash line). The parameters defining the the response at sub-zero temperatures were fixed to values that make the curve reach zero at −5°C based on the continuous chamber data from several years.
Mentions: The initial slope of the light response (αs) and P1200 in the monthly response measurements showed similar relationships between the prevailing ambient temperature and reference temperature (Figure 6). Relative P1200 had slightly steeper slope of the temperature response than αs. Temperatures below zero were not used in the response measurements but the automatic chamber data indicates steep drop in photosynthetic rate below 0°C and the CO2 exchange signal, including respiration, diminishes at about −5°C (data not shown, see model approximation in Figure 6).

Bottom Line: Despite the downregulation, the photosynthetic machinery retained a significant capacity during winter, which was not visible in the field measurements.On the other hand, the operating quantum yield of photosystem II and the initial slope of photosynthetic light response stayed almost at the summertime level until late autumn while at the same time P sat decreased following the prevailing temperature.Comparison of photosynthetic parameters with the environmental drivers suggests that light and minimum temperature are also decisive factors in the seasonal acclimation of photosynthesis in boreal evergreen trees.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics, University of Helsinki Helsinki, Finland.

ABSTRACT
Understanding the seasonality of photosynthesis in boreal evergreen trees and its control by the environment requires separation of the instantaneous and slow responses, as well as the dynamics of light reactions, carbon reactions, and respiration. We determined the seasonality of photosynthetic light response and respiration parameters of Scots pine (Pinus sylvestris L.) in the field in southern Finland and in controlled laboratory conditions. CO2 exchange and chlorophyll fluorescence were measured in the field using a continuously operated automated chamber setup and fluorescence monitoring systems. We also carried out monthly measurements of photosynthetic light, CO2 and temperature responses in standard conditions with a portable IRGA and fluorometer instrument. The field and response measurements indicated strong seasonal variability in the state of the photosynthetic machinery with a deep downregulation during winter. Despite the downregulation, the photosynthetic machinery retained a significant capacity during winter, which was not visible in the field measurements. Light-saturated photosynthesis (P sat) and the initial slope of the photosynthetic light response (α) obtained in standard conditions were up to 20% of their respective summertime values. Respiration also showed seasonal acclimation with peak values of respiration in standard temperature in spring and decline in autumn. Spring recovery of all photosynthetic parameters could be predicted with temperature history. On the other hand, the operating quantum yield of photosystem II and the initial slope of photosynthetic light response stayed almost at the summertime level until late autumn while at the same time P sat decreased following the prevailing temperature. Comparison of photosynthetic parameters with the environmental drivers suggests that light and minimum temperature are also decisive factors in the seasonal acclimation of photosynthesis in boreal evergreen trees.

No MeSH data available.


Related in: MedlinePlus