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Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP.

Phan NV, Sussitz HF, Lieberzeit PA - Biosensors (Basel) (2014)

Bottom Line: Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM).However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses.Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty for Chemistry, University of Vienna, Währinger Straße 38, A-1090 Vienna, Austria; E-Mails: honamd99@yahoo.com (N.V.H.P.); hermann.franz.sussitz@univie.ac.at (H.F.S.).

ABSTRACT
Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.

No MeSH data available.


QCM sensor signals obtained for different ratios of functional monomers; cross linker 70%. The sensor signal shown is the difference in response between MIP and NIP.
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biosensors-04-00161-f005: QCM sensor signals obtained for different ratios of functional monomers; cross linker 70%. The sensor signal shown is the difference in response between MIP and NIP.

Mentions: The outcome for all these materials is summarized in Figure 5. Sensitivity could be increased significantly by using acrylic acid as a monomer rather than methacrylic acid: sensor responses went from 200 Hz to 900 Hz and thus increased by a factor of 4.5. However the response times increased by roughly the same factor. Varying the ratio of the two functional monomers (acrylic acid and vinyl pyrrolidone) has small, but highly significant effects: Functional monomer ratio of 1:1 (w/w) AA:VP leads to a sensor signal of 550 Hz while a ratio 2:3 (w/w) led to 355 Hz. Increasing the amount of vinylpyrrolidone thus reduces sensitivity. However, response times also reached the initial value.


Polymerization Parameters Influencing the QCM Response Characteristics of BSA MIP.

Phan NV, Sussitz HF, Lieberzeit PA - Biosensors (Basel) (2014)

QCM sensor signals obtained for different ratios of functional monomers; cross linker 70%. The sensor signal shown is the difference in response between MIP and NIP.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264377&req=5

biosensors-04-00161-f005: QCM sensor signals obtained for different ratios of functional monomers; cross linker 70%. The sensor signal shown is the difference in response between MIP and NIP.
Mentions: The outcome for all these materials is summarized in Figure 5. Sensitivity could be increased significantly by using acrylic acid as a monomer rather than methacrylic acid: sensor responses went from 200 Hz to 900 Hz and thus increased by a factor of 4.5. However the response times increased by roughly the same factor. Varying the ratio of the two functional monomers (acrylic acid and vinyl pyrrolidone) has small, but highly significant effects: Functional monomer ratio of 1:1 (w/w) AA:VP leads to a sensor signal of 550 Hz while a ratio 2:3 (w/w) led to 355 Hz. Increasing the amount of vinylpyrrolidone thus reduces sensitivity. However, response times also reached the initial value.

Bottom Line: Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM).However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses.Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Analytical Chemistry, Faculty for Chemistry, University of Vienna, Währinger Straße 38, A-1090 Vienna, Austria; E-Mails: honamd99@yahoo.com (N.V.H.P.); hermann.franz.sussitz@univie.ac.at (H.F.S.).

ABSTRACT
Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.

No MeSH data available.