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Hierarchical thin film architectures for enhanced sensor performance: liquid crystal-mediated electrochemical synthesis of nanostructured imprinted polymer films for the selective recognition of bupivacaine.

Suriyanarayanan S, Nawaz H, Ndizeye N, Nicholls IA - Biosensors (Basel) (2014)

Bottom Line: Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water).Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes.The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

View Article: PubMed Central - PubMed

Affiliation: Bioorganic and Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry and Department of Chemistry and Biomedical Sciences, Linnæus University, SE-391 82 Kalmar, Sweden; E-Mails: esusu@lnu.se (S.S.), nawazhazrat@gmail.com (H.N.); natacha.ndizeye@lnu.se (N.N.).

ABSTRACT
Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water). Films prepared in water and in the absence of template were used for control studies. Infrared spectroscopic studies demonstrated comparable chemical compositions for LC and control polymer films. SEM studies revealed that the topologies of the molecularly imprinted polymer films prepared in the LC medium (LC-MIP) exhibit discernible 40 nm thick nano-fiber structures, quite unlike the polymers prepared in the absence of the LC-phase. The sensitivity of the LC-MIP in a quartz crystal microbalance (QCM) sensor platform was 67.6 ± 4.9 Hz/mM under flow injection analysis (FIA) conditions, which was ≈250% higher than for the sensor prepared using the aqueous medium. Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes. The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

No MeSH data available.


Related in: MedlinePlus

Cyclic voltammograms for the electrochemical co-polymerization of 5 mM of (2) and 25 mM of (3) in the presence (A) and absence (B) of 1 mM bupivacaine, in 0.2 M Na2SO4 aqueous solution at pH 8.0 on the gold coated quartz electrode. Potential scan rate was 0.05 V/s. Curves 1 and 2 denotes the first and fifth cycle of the cyclic voltammogram.
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biosensors-04-00090-f007: Cyclic voltammograms for the electrochemical co-polymerization of 5 mM of (2) and 25 mM of (3) in the presence (A) and absence (B) of 1 mM bupivacaine, in 0.2 M Na2SO4 aqueous solution at pH 8.0 on the gold coated quartz electrode. Potential scan rate was 0.05 V/s. Curves 1 and 2 denotes the first and fifth cycle of the cyclic voltammogram.

Mentions: Figure A1: Cyclic voltammograms.


Hierarchical thin film architectures for enhanced sensor performance: liquid crystal-mediated electrochemical synthesis of nanostructured imprinted polymer films for the selective recognition of bupivacaine.

Suriyanarayanan S, Nawaz H, Ndizeye N, Nicholls IA - Biosensors (Basel) (2014)

Cyclic voltammograms for the electrochemical co-polymerization of 5 mM of (2) and 25 mM of (3) in the presence (A) and absence (B) of 1 mM bupivacaine, in 0.2 M Na2SO4 aqueous solution at pH 8.0 on the gold coated quartz electrode. Potential scan rate was 0.05 V/s. Curves 1 and 2 denotes the first and fifth cycle of the cyclic voltammogram.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264373&req=5

biosensors-04-00090-f007: Cyclic voltammograms for the electrochemical co-polymerization of 5 mM of (2) and 25 mM of (3) in the presence (A) and absence (B) of 1 mM bupivacaine, in 0.2 M Na2SO4 aqueous solution at pH 8.0 on the gold coated quartz electrode. Potential scan rate was 0.05 V/s. Curves 1 and 2 denotes the first and fifth cycle of the cyclic voltammogram.
Mentions: Figure A1: Cyclic voltammograms.

Bottom Line: Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water).Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes.The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

View Article: PubMed Central - PubMed

Affiliation: Bioorganic and Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry and Department of Chemistry and Biomedical Sciences, Linnæus University, SE-391 82 Kalmar, Sweden; E-Mails: esusu@lnu.se (S.S.), nawazhazrat@gmail.com (H.N.); natacha.ndizeye@lnu.se (N.N.).

ABSTRACT
Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water). Films prepared in water and in the absence of template were used for control studies. Infrared spectroscopic studies demonstrated comparable chemical compositions for LC and control polymer films. SEM studies revealed that the topologies of the molecularly imprinted polymer films prepared in the LC medium (LC-MIP) exhibit discernible 40 nm thick nano-fiber structures, quite unlike the polymers prepared in the absence of the LC-phase. The sensitivity of the LC-MIP in a quartz crystal microbalance (QCM) sensor platform was 67.6 ± 4.9 Hz/mM under flow injection analysis (FIA) conditions, which was ≈250% higher than for the sensor prepared using the aqueous medium. Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes. The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

No MeSH data available.


Related in: MedlinePlus