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Hierarchical thin film architectures for enhanced sensor performance: liquid crystal-mediated electrochemical synthesis of nanostructured imprinted polymer films for the selective recognition of bupivacaine.

Suriyanarayanan S, Nawaz H, Ndizeye N, Nicholls IA - Biosensors (Basel) (2014)

Bottom Line: Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water).Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes.The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

View Article: PubMed Central - PubMed

Affiliation: Bioorganic and Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry and Department of Chemistry and Biomedical Sciences, Linnæus University, SE-391 82 Kalmar, Sweden; E-Mails: esusu@lnu.se (S.S.), nawazhazrat@gmail.com (H.N.); natacha.ndizeye@lnu.se (N.N.).

ABSTRACT
Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water). Films prepared in water and in the absence of template were used for control studies. Infrared spectroscopic studies demonstrated comparable chemical compositions for LC and control polymer films. SEM studies revealed that the topologies of the molecularly imprinted polymer films prepared in the LC medium (LC-MIP) exhibit discernible 40 nm thick nano-fiber structures, quite unlike the polymers prepared in the absence of the LC-phase. The sensitivity of the LC-MIP in a quartz crystal microbalance (QCM) sensor platform was 67.6 ± 4.9 Hz/mM under flow injection analysis (FIA) conditions, which was ≈250% higher than for the sensor prepared using the aqueous medium. Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes. The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

No MeSH data available.


Related in: MedlinePlus

Variation in the resonant frequency of gold-coated quartz resonators overlaid with (A) MIP and (B) REF films prepared in LC medium, upon injection of analyte (1) under FIA condition. Inset is the corresponding FIA calibration plots for 1 on MIP and REF film. Phosphate buffer (0.01 M) containing 150 mM NaCl at pH = 8.5 was used as carrier solution at the flow rate of 25 µL/min. The injection volume was 75 µL.
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biosensors-04-00090-f004: Variation in the resonant frequency of gold-coated quartz resonators overlaid with (A) MIP and (B) REF films prepared in LC medium, upon injection of analyte (1) under FIA condition. Inset is the corresponding FIA calibration plots for 1 on MIP and REF film. Phosphate buffer (0.01 M) containing 150 mM NaCl at pH = 8.5 was used as carrier solution at the flow rate of 25 µL/min. The injection volume was 75 µL.

Mentions: In Figure 4, the QCM traces (resonant frequency versus time) from the injection of 1 over the LC-MIP and REF film coated resonators at concentrations from 0.3 to 2 mM are shown. The response time, the time taken by the signal to reach 90% of its maximum value, was as short as ≈70 s. The sensor surface was recovered by subsequent washing with the running buffer solution until the frequency had been returned to its initial value, 6.5 min. The responses were significantly greater for the MIP surface than the corresponding reference. This was in contrast to the bupivacaine-MIP films prepared in aqueous conditions; which had lower values for both response and recovery times, ≈100 s and 8.3 min, respectively (Figure A4). Collectively these results demonstrate that the hierarchical architectural features present in the MIP film prepared in the presence of the LC medium lead to an enhancement of sensor performance.


Hierarchical thin film architectures for enhanced sensor performance: liquid crystal-mediated electrochemical synthesis of nanostructured imprinted polymer films for the selective recognition of bupivacaine.

Suriyanarayanan S, Nawaz H, Ndizeye N, Nicholls IA - Biosensors (Basel) (2014)

Variation in the resonant frequency of gold-coated quartz resonators overlaid with (A) MIP and (B) REF films prepared in LC medium, upon injection of analyte (1) under FIA condition. Inset is the corresponding FIA calibration plots for 1 on MIP and REF film. Phosphate buffer (0.01 M) containing 150 mM NaCl at pH = 8.5 was used as carrier solution at the flow rate of 25 µL/min. The injection volume was 75 µL.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264373&req=5

biosensors-04-00090-f004: Variation in the resonant frequency of gold-coated quartz resonators overlaid with (A) MIP and (B) REF films prepared in LC medium, upon injection of analyte (1) under FIA condition. Inset is the corresponding FIA calibration plots for 1 on MIP and REF film. Phosphate buffer (0.01 M) containing 150 mM NaCl at pH = 8.5 was used as carrier solution at the flow rate of 25 µL/min. The injection volume was 75 µL.
Mentions: In Figure 4, the QCM traces (resonant frequency versus time) from the injection of 1 over the LC-MIP and REF film coated resonators at concentrations from 0.3 to 2 mM are shown. The response time, the time taken by the signal to reach 90% of its maximum value, was as short as ≈70 s. The sensor surface was recovered by subsequent washing with the running buffer solution until the frequency had been returned to its initial value, 6.5 min. The responses were significantly greater for the MIP surface than the corresponding reference. This was in contrast to the bupivacaine-MIP films prepared in aqueous conditions; which had lower values for both response and recovery times, ≈100 s and 8.3 min, respectively (Figure A4). Collectively these results demonstrate that the hierarchical architectural features present in the MIP film prepared in the presence of the LC medium lead to an enhancement of sensor performance.

Bottom Line: Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water).Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes.The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

View Article: PubMed Central - PubMed

Affiliation: Bioorganic and Biophysical Chemistry Laboratory, Linnæus University Centre for Biomaterials Chemistry and Department of Chemistry and Biomedical Sciences, Linnæus University, SE-391 82 Kalmar, Sweden; E-Mails: esusu@lnu.se (S.S.), nawazhazrat@gmail.com (H.N.); natacha.ndizeye@lnu.se (N.N.).

ABSTRACT
Nanostructured bupivacaine-selective molecularly imprinted 3-aminophenylboronic acid-p-phenylenediamine co-polymer (MIP) films have been prepared on gold-coated quartz (Au/quartz) resonators by electrochemical synthesis under cyclic voltammetric conditions in a liquid crystalline (LC) medium (triton X-100/water). Films prepared in water and in the absence of template were used for control studies. Infrared spectroscopic studies demonstrated comparable chemical compositions for LC and control polymer films. SEM studies revealed that the topologies of the molecularly imprinted polymer films prepared in the LC medium (LC-MIP) exhibit discernible 40 nm thick nano-fiber structures, quite unlike the polymers prepared in the absence of the LC-phase. The sensitivity of the LC-MIP in a quartz crystal microbalance (QCM) sensor platform was 67.6 ± 4.9 Hz/mM under flow injection analysis (FIA) conditions, which was ≈250% higher than for the sensor prepared using the aqueous medium. Detection was possible at 100 nM (30 ng/mL), and discrimination of bupivacaine from closely related structural analogs was readily achieved as reflected in the corresponding stability constants of the MIP-analyte complexes. The facile fabrication and significant enhancement in sensor sensitivity together highlight the potential of this LC-based imprinting strategy for fabrication of polymeric materials with hierarchical architectures, in particular for use in surface-dependent application areas, e.g., biomaterials or sensing.

No MeSH data available.


Related in: MedlinePlus