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Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis.

Li Y, Burke DT, Kopelman R, Burns MA - Biosensors (Basel) (2014)

Bottom Line: Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length.The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range.The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; E-Mail: yunzili@umich.edu.

ABSTRACT
We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.

No MeSH data available.


Plot of reaction cycle number versus log of initial DNA amount for the qPCR measurement by AMBR method. Error bars represent the uncertainty due to the AMBR measurement of every five cycles.
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biosensors-04-00076-f007: Plot of reaction cycle number versus log of initial DNA amount for the qPCR measurement by AMBR method. Error bars represent the uncertainty due to the AMBR measurement of every five cycles.


Asynchronous Magnetic Bead Rotation (AMBR) Microviscometer for Label-Free DNA Analysis.

Li Y, Burke DT, Kopelman R, Burns MA - Biosensors (Basel) (2014)

Plot of reaction cycle number versus log of initial DNA amount for the qPCR measurement by AMBR method. Error bars represent the uncertainty due to the AMBR measurement of every five cycles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264372&req=5

biosensors-04-00076-f007: Plot of reaction cycle number versus log of initial DNA amount for the qPCR measurement by AMBR method. Error bars represent the uncertainty due to the AMBR measurement of every five cycles.
Bottom Line: Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length.The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range.The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; E-Mail: yunzili@umich.edu.

ABSTRACT
We have developed a label-free viscosity-based DNA detection system, using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. We have demonstrated experimentally that the bead rotation period is linearly proportional to the viscosity of a DNA solution surrounding the paramagnetic bead, as expected theoretically. Simple optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of DNA concentration or average fragment length. The response of the AMBR microviscometer yields reproducible measurement of DNA solutions, enzymatic digestion reactions, and PCR systems at template concentrations across a 5000-fold range. The results demonstrate the feasibility of viscosity-based DNA detection using AMBR in microscale aqueous volumes.

No MeSH data available.