Limits...
ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin.

Ibupoto ZH, Ali SM, Khun K, Chey CO, Nur O, Willander M - Biosensors (Basel) (2011)

Bottom Line: In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method.During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin.A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. zafar.hussain.ibupoto@liu.se.

ABSTRACT
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

No MeSH data available.


The time response curve of the proposed sensor in a 1,000 µM penicillin electrolytic test solution in presence of interfering species.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4264348&req=5

biosensors-01-00153-f006: The time response curve of the proposed sensor in a 1,000 µM penicillin electrolytic test solution in presence of interfering species.

Mentions: The selectivity is an important parameter for the performance evaluation of biosensors, as it gives the particular affinity for observing specific target ions in the presence of other interfering ions. The selectivity of any biosensor can be evaluated in an electrolyte test solution by adding other interfering ions. The enzyme penicillinase has quite acceptable selectivity [40]. For penicillin, the enzyme-analyte reaction is highly specific and produced the charged ions which were selectively determined by our proposed sensor. The penicillinase is very particular in reaction with penicillin, even in presence of other interfering species. We found no significant interference of sensor with Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins. In Figure 6, it can be seen that upon mixing all above interfering components (100 µM of each) into 1 mM of our penicillin electrolyte solution, no changes on the output signal stability or magnitude was observed. Moreover, the sensor also exhibited a very fast response time of less than 5 s when the signal reached its steady state stable value.


ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin.

Ibupoto ZH, Ali SM, Khun K, Chey CO, Nur O, Willander M - Biosensors (Basel) (2011)

The time response curve of the proposed sensor in a 1,000 µM penicillin electrolytic test solution in presence of interfering species.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264348&req=5

biosensors-01-00153-f006: The time response curve of the proposed sensor in a 1,000 µM penicillin electrolytic test solution in presence of interfering species.
Mentions: The selectivity is an important parameter for the performance evaluation of biosensors, as it gives the particular affinity for observing specific target ions in the presence of other interfering ions. The selectivity of any biosensor can be evaluated in an electrolyte test solution by adding other interfering ions. The enzyme penicillinase has quite acceptable selectivity [40]. For penicillin, the enzyme-analyte reaction is highly specific and produced the charged ions which were selectively determined by our proposed sensor. The penicillinase is very particular in reaction with penicillin, even in presence of other interfering species. We found no significant interference of sensor with Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins. In Figure 6, it can be seen that upon mixing all above interfering components (100 µM of each) into 1 mM of our penicillin electrolyte solution, no changes on the output signal stability or magnitude was observed. Moreover, the sensor also exhibited a very fast response time of less than 5 s when the signal reached its steady state stable value.

Bottom Line: In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method.During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin.A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

View Article: PubMed Central - PubMed

Affiliation: Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden. zafar.hussain.ibupoto@liu.se.

ABSTRACT
In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.

No MeSH data available.