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Variation in time and magnitude of immune response and viremia in experimental challenges with Porcine circovirus 2b.

Engle TB, Jobman EE, Moural TW, McKnite AM, Bundy JW, Barnes SY, Davis EH, Galeota JA, Burkey TE, Plastow GS, Kachman SD, Ciobanu DC - BMC Vet. Res. (2014)

Bottom Line: In contrast, PCV2 viremia was an important driver of ADG decline following infection; a moderate negative correlation was observed between viral load and overall ADG (r = - 0.35, P < 0.001).In addition, the highly viremic group expressed higher IgM and IgG starting with d 14 and d 21 respectively, and higher tumor necrosis factor - alpha (TNF-α) at d 21 (P < 0.005), compared to low viremic group.Molecular sources of the observed differences in viremia and immune response could provide a better understanding of the host factors that influence the development of PCVAD and lead to improved knowledge of swine immunity.

View Article: PubMed Central - PubMed

Affiliation: Animal Science Department, University of Nebraska, Lincoln, NE, 68583-0908, USA. tbengle@huskers.unl.edu.

ABSTRACT

Background: Porcine circovirus 2 is the primary agent responsible for inducing a group of associated diseases known as Porcine Circovirus Associated Diseases (PCVAD), which can have detrimental effects on production efficiency as well as causing significant mortality. The objective of this study was to evaluate variation in viral replication, immune response and growth across pigs (n = 974) from different crossbred lines. The approach used in this study was experimental infection with a PCV2b strain of pigs at an average of 43 days of age.

Results: The sequence of the PCV2b isolate used in the challenge was similar with a cluster of PCV2b isolates known to induce PCVAD and increased mortality rates. The swine leukocyte antigen class II (SLAII) profile of the population was diverse, with nine DQB1 haplotypes being present. Individual viremia and antibody profiles during challenge demonstrate variation in magnitude and time of viral surge and immune response. The correlations between PCV2 specific antibodies and average daily gain (ADG) were relatively low and varied between - 0.14 to 0.08 for IgM and -0.02 and 0.11 for IgG. In contrast, PCV2 viremia was an important driver of ADG decline following infection; a moderate negative correlation was observed between viral load and overall ADG (r = - 0.35, P < 0.001). The pigs with the lowest 10% level of viral load maintained a steady increase in weekly ADG (P < 0.0001) compared to the pigs that had the 10% greatest viral load (P < 0.55). In addition, the highly viremic group expressed higher IgM and IgG starting with d 14 and d 21 respectively, and higher tumor necrosis factor - alpha (TNF-α) at d 21 (P < 0.005), compared to low viremic group.

Conclusions: Molecular sources of the observed differences in viremia and immune response could provide a better understanding of the host factors that influence the development of PCVAD and lead to improved knowledge of swine immunity.

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Variation across time points in PCV2b viremia*following experimental challenge with PCV2b. *measured as number of PCV2 genome copy number/ml of serum (log10).
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Fig3: Variation across time points in PCV2b viremia*following experimental challenge with PCV2b. *measured as number of PCV2 genome copy number/ml of serum (log10).

Mentions: The wk 3 and wk 4 were the weeks associated with the largest variation in ADG following PCV2b challenge (Table 2). In the last week of challenge the pigs exhibited the largest average ADG, as the immune system started to clear the virus (Figure 2). Previous studies showed that PCV2 vaccinated pigs exhibit consistent increased in ADG during this development period [18]. In this study, average ADG across time points indicates a deviation from this steady increased in weekly ADG due to a plateau in growth observed during wk 3 (Figure 2). This plateau could be a result of viral replication since it coincides with the days characterized by the largest viremia levels (Figure 3).Table 2


Variation in time and magnitude of immune response and viremia in experimental challenges with Porcine circovirus 2b.

Engle TB, Jobman EE, Moural TW, McKnite AM, Bundy JW, Barnes SY, Davis EH, Galeota JA, Burkey TE, Plastow GS, Kachman SD, Ciobanu DC - BMC Vet. Res. (2014)

Variation across time points in PCV2b viremia*following experimental challenge with PCV2b. *measured as number of PCV2 genome copy number/ml of serum (log10).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4264338&req=5

Fig3: Variation across time points in PCV2b viremia*following experimental challenge with PCV2b. *measured as number of PCV2 genome copy number/ml of serum (log10).
Mentions: The wk 3 and wk 4 were the weeks associated with the largest variation in ADG following PCV2b challenge (Table 2). In the last week of challenge the pigs exhibited the largest average ADG, as the immune system started to clear the virus (Figure 2). Previous studies showed that PCV2 vaccinated pigs exhibit consistent increased in ADG during this development period [18]. In this study, average ADG across time points indicates a deviation from this steady increased in weekly ADG due to a plateau in growth observed during wk 3 (Figure 2). This plateau could be a result of viral replication since it coincides with the days characterized by the largest viremia levels (Figure 3).Table 2

Bottom Line: In contrast, PCV2 viremia was an important driver of ADG decline following infection; a moderate negative correlation was observed between viral load and overall ADG (r = - 0.35, P < 0.001).In addition, the highly viremic group expressed higher IgM and IgG starting with d 14 and d 21 respectively, and higher tumor necrosis factor - alpha (TNF-α) at d 21 (P < 0.005), compared to low viremic group.Molecular sources of the observed differences in viremia and immune response could provide a better understanding of the host factors that influence the development of PCVAD and lead to improved knowledge of swine immunity.

View Article: PubMed Central - PubMed

Affiliation: Animal Science Department, University of Nebraska, Lincoln, NE, 68583-0908, USA. tbengle@huskers.unl.edu.

ABSTRACT

Background: Porcine circovirus 2 is the primary agent responsible for inducing a group of associated diseases known as Porcine Circovirus Associated Diseases (PCVAD), which can have detrimental effects on production efficiency as well as causing significant mortality. The objective of this study was to evaluate variation in viral replication, immune response and growth across pigs (n = 974) from different crossbred lines. The approach used in this study was experimental infection with a PCV2b strain of pigs at an average of 43 days of age.

Results: The sequence of the PCV2b isolate used in the challenge was similar with a cluster of PCV2b isolates known to induce PCVAD and increased mortality rates. The swine leukocyte antigen class II (SLAII) profile of the population was diverse, with nine DQB1 haplotypes being present. Individual viremia and antibody profiles during challenge demonstrate variation in magnitude and time of viral surge and immune response. The correlations between PCV2 specific antibodies and average daily gain (ADG) were relatively low and varied between - 0.14 to 0.08 for IgM and -0.02 and 0.11 for IgG. In contrast, PCV2 viremia was an important driver of ADG decline following infection; a moderate negative correlation was observed between viral load and overall ADG (r = - 0.35, P < 0.001). The pigs with the lowest 10% level of viral load maintained a steady increase in weekly ADG (P < 0.0001) compared to the pigs that had the 10% greatest viral load (P < 0.55). In addition, the highly viremic group expressed higher IgM and IgG starting with d 14 and d 21 respectively, and higher tumor necrosis factor - alpha (TNF-α) at d 21 (P < 0.005), compared to low viremic group.

Conclusions: Molecular sources of the observed differences in viremia and immune response could provide a better understanding of the host factors that influence the development of PCVAD and lead to improved knowledge of swine immunity.

Show MeSH
Related in: MedlinePlus