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BIIDXI, the At4g32460 DUF642 gene, is involved in pectin methyl esterase regulation during Arabidopsis thaliana seed germination and plant development.

Zúñiga-Sánchez E, Soriano D, Martínez-Barajas E, Orozco-Segovia A, Gamboa-deBuen A - BMC Plant Biol. (2014)

Bottom Line: Transgenic lines overexpressing these proteins showed increased PME activity during seed germination, and improved seed germination performance.In plants expressing At4g32460 antisense RNA, PME activity was decreased in the leaves, and the siliques were very short and contained no seeds.Our results suggested that the DUF642 family contributes to the complexity of the methylesterification process by participating in the fine regulation of pectin status during plant development.

View Article: PubMed Central - PubMed

ABSTRACT

Background: DUF642 proteins constitute a highly conserved family of proteins that are associated with the cell wall and are specific to spermatophytes. Transcriptome studies have suggested that members of this family are involved in seed development and germination processes. Previous in vitro studies have revealed that At4g32460- and At5g11420-encoded proteins interact with the catalytic domain of pectin methyl esterase 3 (AtPME3, which is encoded by At3g14310). PMEs play an important role in plant development, including seed germination. The aim of this study was to evaluate the function of the DUF642 gene At4g32460 during seed germination and plant development and to determine its relation to PME activity regulation.

Results: Our results indicated that the DUF642 proteins encoded by At4g32460 and At5g11420 could be positive regulators of PME activity during several developmental processes. Transgenic lines overexpressing these proteins showed increased PME activity during seed germination, and improved seed germination performance. In plants expressing At4g32460 antisense RNA, PME activity was decreased in the leaves, and the siliques were very short and contained no seeds. This phenotype was also present in the SALK_142260 and SALK_054867 lines for At4g32460.

Conclusions: Our results suggested that the DUF642 family contributes to the complexity of the methylesterification process by participating in the fine regulation of pectin status during plant development.

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Related in: MedlinePlus

PME activity during germination of OEBDX and OE11420 control (dry) seeds. A) Cumulative testa rupture curve. B) Cumulative endosperm rupture curve. C) PME activity. PME activity assays were performed in triplicate. All experiments were performed at least twice for each line, with different seed lots. For each time point, letters indicate significant differences. Error bars show SE.
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Fig5: PME activity during germination of OEBDX and OE11420 control (dry) seeds. A) Cumulative testa rupture curve. B) Cumulative endosperm rupture curve. C) PME activity. PME activity assays were performed in triplicate. All experiments were performed at least twice for each line, with different seed lots. For each time point, letters indicate significant differences. Error bars show SE.

Mentions: To determine the effects of the overexpressed genes on seed germination and PME activity during germination, we tested seeds without a priming pre-treatment. The initial testa rupture and endosperm rupture times were shorter for OE seeds than for wt seeds (p =0.01 and p < 0.01, respectively). The seeds of OEBDX and OE11420 did not show different testa rupture rates, but seeds of both lines showed significantly lower endosperm rupture rates compared with that of wt seeds (p =0.01, Figure 5A and B). For wt seeds, PME activity increased before testa rupture was complete and decreased thereafter, similar to the pattern reported previously [13]. The same pattern of PME activity was observed in OE11420 seeds. At 20 h of germination, PME activity was significantly higher in OE11420 seeds than in wt seeds. However, the PME activity pattern for OEBDX seeds was different; the PME activity did not decrease at 34 h of germination. In the 1-h and 34-h germinating seeds, PME activity was higher in OEBDX seeds than in wt seeds (p <0.001, Figure 5C).Figure 5


BIIDXI, the At4g32460 DUF642 gene, is involved in pectin methyl esterase regulation during Arabidopsis thaliana seed germination and plant development.

Zúñiga-Sánchez E, Soriano D, Martínez-Barajas E, Orozco-Segovia A, Gamboa-deBuen A - BMC Plant Biol. (2014)

PME activity during germination of OEBDX and OE11420 control (dry) seeds. A) Cumulative testa rupture curve. B) Cumulative endosperm rupture curve. C) PME activity. PME activity assays were performed in triplicate. All experiments were performed at least twice for each line, with different seed lots. For each time point, letters indicate significant differences. Error bars show SE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4264326&req=5

Fig5: PME activity during germination of OEBDX and OE11420 control (dry) seeds. A) Cumulative testa rupture curve. B) Cumulative endosperm rupture curve. C) PME activity. PME activity assays were performed in triplicate. All experiments were performed at least twice for each line, with different seed lots. For each time point, letters indicate significant differences. Error bars show SE.
Mentions: To determine the effects of the overexpressed genes on seed germination and PME activity during germination, we tested seeds without a priming pre-treatment. The initial testa rupture and endosperm rupture times were shorter for OE seeds than for wt seeds (p =0.01 and p < 0.01, respectively). The seeds of OEBDX and OE11420 did not show different testa rupture rates, but seeds of both lines showed significantly lower endosperm rupture rates compared with that of wt seeds (p =0.01, Figure 5A and B). For wt seeds, PME activity increased before testa rupture was complete and decreased thereafter, similar to the pattern reported previously [13]. The same pattern of PME activity was observed in OE11420 seeds. At 20 h of germination, PME activity was significantly higher in OE11420 seeds than in wt seeds. However, the PME activity pattern for OEBDX seeds was different; the PME activity did not decrease at 34 h of germination. In the 1-h and 34-h germinating seeds, PME activity was higher in OEBDX seeds than in wt seeds (p <0.001, Figure 5C).Figure 5

Bottom Line: Transgenic lines overexpressing these proteins showed increased PME activity during seed germination, and improved seed germination performance.In plants expressing At4g32460 antisense RNA, PME activity was decreased in the leaves, and the siliques were very short and contained no seeds.Our results suggested that the DUF642 family contributes to the complexity of the methylesterification process by participating in the fine regulation of pectin status during plant development.

View Article: PubMed Central - PubMed

ABSTRACT

Background: DUF642 proteins constitute a highly conserved family of proteins that are associated with the cell wall and are specific to spermatophytes. Transcriptome studies have suggested that members of this family are involved in seed development and germination processes. Previous in vitro studies have revealed that At4g32460- and At5g11420-encoded proteins interact with the catalytic domain of pectin methyl esterase 3 (AtPME3, which is encoded by At3g14310). PMEs play an important role in plant development, including seed germination. The aim of this study was to evaluate the function of the DUF642 gene At4g32460 during seed germination and plant development and to determine its relation to PME activity regulation.

Results: Our results indicated that the DUF642 proteins encoded by At4g32460 and At5g11420 could be positive regulators of PME activity during several developmental processes. Transgenic lines overexpressing these proteins showed increased PME activity during seed germination, and improved seed germination performance. In plants expressing At4g32460 antisense RNA, PME activity was decreased in the leaves, and the siliques were very short and contained no seeds. This phenotype was also present in the SALK_142260 and SALK_054867 lines for At4g32460.

Conclusions: Our results suggested that the DUF642 family contributes to the complexity of the methylesterification process by participating in the fine regulation of pectin status during plant development.

Show MeSH
Related in: MedlinePlus