Limits...
Detoxification of Croton tiglium L. seeds by Ayurvedic process of Śodhana.

Pal PK, Nandi MK, Singh NK - Anc Sci Life (2014)

Bottom Line: Croton tiglium seeds, known as Jamālgoṭa in Hindi, Marathi, and Urdu is well-known for its toxicity (severe purgative action).Phorbol esters equivalent to phorbol-12-myristate-13-acetate (PMA) and crotonic acid contents were quantified by high-performance liquid chromatography method in the seeds of C. tiglium before and after the purification process.These constituents are oil soluble and may be removed by cow milk during the process of Śodhana.

View Article: PubMed Central - PubMed

Affiliation: Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Barkachha, Mirzapur, India.

ABSTRACT

Objective: Croton tiglium seeds, known as Jamālgoṭa in Hindi, Marathi, and Urdu is well-known for its toxicity (severe purgative action). In Ayurvedic texts, the plant is known as Kumbhinī and is used for the treatment of constipation after Śodhana (detoxification process) of the seeds with Godugdha (cow milk).

Material and methods: In the present study, C. tiglium seeds were purified with cow milk as reported in Ayurvedic classics. Phorbol esters equivalent to phorbol-12-myristate-13-acetate (PMA) and crotonic acid contents were quantified by high-performance liquid chromatography method in the seeds of C. tiglium before and after the purification process.

Results: The content of the phorbol ester equivalent to PMA in unpurified and purified sample was found to be 5.2 mg/100 g and 1.8 mg/100 g of dried seeds of C. tiglium, respectively. The quantity of crotonic acid in unpurified seeds of C. tiglium was found to be 0.102 mg/100 g of dried seeds while it was absent in the purified seed extract of C. tiglium.

Conclusion: The toxicity of C. tiglium seeds may be due to the presence of phorbol esters and crotonic acid along with other constituents. These constituents are oil soluble and may be removed by cow milk during the process of Śodhana. Reduction in the level of these constituents after the purification decreases the toxicity of C. tiglium seeds. Reduction in the oily content from the seeds of C. tiglium during the purification process is also supported by the results obtained from the physiochemical parameters.

No MeSH data available.


Related in: MedlinePlus

High-performance liquid chromatography of unpurified seed extract of Croton tiglium for phorbol-12-myristate-13-acetate
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4264303&req=5

Figure 1: High-performance liquid chromatography of unpurified seed extract of Croton tiglium for phorbol-12-myristate-13-acetate

Mentions: Phorbol ester was quantified by HPLC method developed by Hass and Mittelbach[14] and modified by Saetae and Suntornsuk.[15] The analytical column used was a Zorbax, C18 (4 μm particle size and 150 mm × 3.9 mm internal diameter) column. The column was thermally controlled at 25°C. A mixture of acetonitrile and deionized water (80:20, v: v) was used as the mobile phase at a flow rate of 1 ml/min. The diode array detector wavelength was set at 254 nm. The PMA was used as an external standard [Figures 1 and 2].


Detoxification of Croton tiglium L. seeds by Ayurvedic process of Śodhana.

Pal PK, Nandi MK, Singh NK - Anc Sci Life (2014)

High-performance liquid chromatography of unpurified seed extract of Croton tiglium for phorbol-12-myristate-13-acetate
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264303&req=5

Figure 1: High-performance liquid chromatography of unpurified seed extract of Croton tiglium for phorbol-12-myristate-13-acetate
Mentions: Phorbol ester was quantified by HPLC method developed by Hass and Mittelbach[14] and modified by Saetae and Suntornsuk.[15] The analytical column used was a Zorbax, C18 (4 μm particle size and 150 mm × 3.9 mm internal diameter) column. The column was thermally controlled at 25°C. A mixture of acetonitrile and deionized water (80:20, v: v) was used as the mobile phase at a flow rate of 1 ml/min. The diode array detector wavelength was set at 254 nm. The PMA was used as an external standard [Figures 1 and 2].

Bottom Line: Croton tiglium seeds, known as Jamālgoṭa in Hindi, Marathi, and Urdu is well-known for its toxicity (severe purgative action).Phorbol esters equivalent to phorbol-12-myristate-13-acetate (PMA) and crotonic acid contents were quantified by high-performance liquid chromatography method in the seeds of C. tiglium before and after the purification process.These constituents are oil soluble and may be removed by cow milk during the process of Śodhana.

View Article: PubMed Central - PubMed

Affiliation: Ayurvedic Pharmacy Laboratory, Rajiv Gandhi South Campus, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Barkachha, Mirzapur, India.

ABSTRACT

Objective: Croton tiglium seeds, known as Jamālgoṭa in Hindi, Marathi, and Urdu is well-known for its toxicity (severe purgative action). In Ayurvedic texts, the plant is known as Kumbhinī and is used for the treatment of constipation after Śodhana (detoxification process) of the seeds with Godugdha (cow milk).

Material and methods: In the present study, C. tiglium seeds were purified with cow milk as reported in Ayurvedic classics. Phorbol esters equivalent to phorbol-12-myristate-13-acetate (PMA) and crotonic acid contents were quantified by high-performance liquid chromatography method in the seeds of C. tiglium before and after the purification process.

Results: The content of the phorbol ester equivalent to PMA in unpurified and purified sample was found to be 5.2 mg/100 g and 1.8 mg/100 g of dried seeds of C. tiglium, respectively. The quantity of crotonic acid in unpurified seeds of C. tiglium was found to be 0.102 mg/100 g of dried seeds while it was absent in the purified seed extract of C. tiglium.

Conclusion: The toxicity of C. tiglium seeds may be due to the presence of phorbol esters and crotonic acid along with other constituents. These constituents are oil soluble and may be removed by cow milk during the process of Śodhana. Reduction in the level of these constituents after the purification decreases the toxicity of C. tiglium seeds. Reduction in the oily content from the seeds of C. tiglium during the purification process is also supported by the results obtained from the physiochemical parameters.

No MeSH data available.


Related in: MedlinePlus