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Hepatoma-derived growth factor upregulation is correlated with prognostic factors of early-stage cervical adenocarcinoma.

Tsai CC, Huang SC, Tai MH, Chien CC, Huang CC, Hsu YC - Int J Mol Sci (2014)

Bottom Line: The expression of HDGF was significantly increased compared with adjacent non-tumor tissue samples (p < 0.001).The HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells compared with primary ones.Cytoplasmic HDGF expression is strongly correlated with pelvic lymph node metastasis and recurrence, indicating that HDGF may serve as a novel prognostic marker for patients with Cx.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kaohsiung and College of Medicine, Chang Gung University, Kaohsiung 83301, Taiwan. nick@cgmh.org.tw.

ABSTRACT
Hepatoma-derived growth factor (HDGF) is a unique nuclear/growth factor that plays an important role in the progression of different types of cancer. A total of 63 patients with early-stage cervical adenocarcinoma (Cx) were enrolled in this retrospective study. The expression of HDGF was significantly increased compared with adjacent non-tumor tissue samples (p < 0.001). Moreover, elevated nuclear HDGF levels were correlated with lymph-vascular space invasion (LVSI; p < 0.05), lymph node metastasis (LNM; p < 0.001), recurrence (p < 0.001) and advanced grade (AG; p < 0.001). The growth of cervical cancer cells (Hela cells) was enhanced by HDGF treatment. The HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells compared with primary ones. By adenovirus gene delivery, HDGF overexpression enhanced, whereas HDGF knockdown perturbed the tumorigenic behaviors of cervical cancer cells. HDGF overexpression is common in early-stage cervical adenocarcinoma and is involved in the carcinogenesis of cervical adenocarcinoma. Cytoplasmic HDGF expression is strongly correlated with pelvic lymph node metastasis and recurrence, indicating that HDGF may serve as a novel prognostic marker for patients with Cx.

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HDGF mediated the survival of cervical cancer cells, thereby increasing proliferation/growth. Correlation of HDGF expression with the tumorigenic behaviors of cervical cancer cells. (A) qRT-PCR and (B) immunoblot analysis of HDGF protein level in human cervical cell lines. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences determined at the level of *p < 0.05 and **p < 0.01 versus the control group (HDGF 0 nM group).
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ijms-15-21492-f003: HDGF mediated the survival of cervical cancer cells, thereby increasing proliferation/growth. Correlation of HDGF expression with the tumorigenic behaviors of cervical cancer cells. (A) qRT-PCR and (B) immunoblot analysis of HDGF protein level in human cervical cell lines. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences determined at the level of *p < 0.05 and **p < 0.01 versus the control group (HDGF 0 nM group).

Mentions: To investigate the role of HDGF during cervical carcinogenesis, we examined HDGF expression in human cervical cell lines and found that the HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells (SiHa and HeLa cells) than in benign ones (primary cervical cancer cells; PCCCs #1 and #2; Figure 3A,B, *p < 0.05 and **p < 0.01).


Hepatoma-derived growth factor upregulation is correlated with prognostic factors of early-stage cervical adenocarcinoma.

Tsai CC, Huang SC, Tai MH, Chien CC, Huang CC, Hsu YC - Int J Mol Sci (2014)

HDGF mediated the survival of cervical cancer cells, thereby increasing proliferation/growth. Correlation of HDGF expression with the tumorigenic behaviors of cervical cancer cells. (A) qRT-PCR and (B) immunoblot analysis of HDGF protein level in human cervical cell lines. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences determined at the level of *p < 0.05 and **p < 0.01 versus the control group (HDGF 0 nM group).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264238&req=5

ijms-15-21492-f003: HDGF mediated the survival of cervical cancer cells, thereby increasing proliferation/growth. Correlation of HDGF expression with the tumorigenic behaviors of cervical cancer cells. (A) qRT-PCR and (B) immunoblot analysis of HDGF protein level in human cervical cell lines. All data are reported as the mean (±SEM) of at least three separate experiments. Statistical analysis was performed using a t-test, with significant differences determined at the level of *p < 0.05 and **p < 0.01 versus the control group (HDGF 0 nM group).
Mentions: To investigate the role of HDGF during cervical carcinogenesis, we examined HDGF expression in human cervical cell lines and found that the HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells (SiHa and HeLa cells) than in benign ones (primary cervical cancer cells; PCCCs #1 and #2; Figure 3A,B, *p < 0.05 and **p < 0.01).

Bottom Line: The expression of HDGF was significantly increased compared with adjacent non-tumor tissue samples (p < 0.001).The HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells compared with primary ones.Cytoplasmic HDGF expression is strongly correlated with pelvic lymph node metastasis and recurrence, indicating that HDGF may serve as a novel prognostic marker for patients with Cx.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Kaohsiung and College of Medicine, Chang Gung University, Kaohsiung 83301, Taiwan. nick@cgmh.org.tw.

ABSTRACT
Hepatoma-derived growth factor (HDGF) is a unique nuclear/growth factor that plays an important role in the progression of different types of cancer. A total of 63 patients with early-stage cervical adenocarcinoma (Cx) were enrolled in this retrospective study. The expression of HDGF was significantly increased compared with adjacent non-tumor tissue samples (p < 0.001). Moreover, elevated nuclear HDGF levels were correlated with lymph-vascular space invasion (LVSI; p < 0.05), lymph node metastasis (LNM; p < 0.001), recurrence (p < 0.001) and advanced grade (AG; p < 0.001). The growth of cervical cancer cells (Hela cells) was enhanced by HDGF treatment. The HDGF mRNA and protein level were significantly higher in malignant cervical cancer cells compared with primary ones. By adenovirus gene delivery, HDGF overexpression enhanced, whereas HDGF knockdown perturbed the tumorigenic behaviors of cervical cancer cells. HDGF overexpression is common in early-stage cervical adenocarcinoma and is involved in the carcinogenesis of cervical adenocarcinoma. Cytoplasmic HDGF expression is strongly correlated with pelvic lymph node metastasis and recurrence, indicating that HDGF may serve as a novel prognostic marker for patients with Cx.

Show MeSH
Related in: MedlinePlus