Limits...
Nicotinic acid increases adiponectin secretion from differentiated bovine preadipocytes through G-protein coupled receptor signaling.

Kopp C, Hosseini A, Singh SP, Regenhard P, Khalilvandi-Behroozyar H, Sauerwein H, Mielenz M - Int J Mol Sci (2014)

Bottom Line: Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle.Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h.The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. christina.kopp@uni-bonn.de.

ABSTRACT
The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.

Show MeSH

Related in: MedlinePlus

The effects of nicotinic acid (NA) on the mRNA abundance of G-protein coupled receptor 109A (GPR109A) in differentiated bovine adipocytes cells (n = 10). After 4 h of starvation, the adipocytes were pre-incubated with (PTX (+)) or without pertussis toxin (PTX (−)) (100 ng/mL) for 16 h and then treated for 12 or 24 h with 10 or 15 µM NA or PBS (control). Due to the absence of differences, the PTX (+) and PTX (−) groups were merged for the analyses of the mRNA abundances. Significant differences (p ≤ 0.05) and trends (p ≤ 0.1) are designated with the corresponding p-values.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4264232&req=5

ijms-15-21401-f003: The effects of nicotinic acid (NA) on the mRNA abundance of G-protein coupled receptor 109A (GPR109A) in differentiated bovine adipocytes cells (n = 10). After 4 h of starvation, the adipocytes were pre-incubated with (PTX (+)) or without pertussis toxin (PTX (−)) (100 ng/mL) for 16 h and then treated for 12 or 24 h with 10 or 15 µM NA or PBS (control). Due to the absence of differences, the PTX (+) and PTX (−) groups were merged for the analyses of the mRNA abundances. Significant differences (p ≤ 0.05) and trends (p ≤ 0.1) are designated with the corresponding p-values.

Mentions: Pre-incubation with PTX had no effect on the mRNA abundances of AdipoR1/2, FABP4, or GPR109A. Therefore, the PTX (+) and PTX (−) groups were merged for further analyses. Compared to the controls, incubation for 24 h with 15 µM NA increased the mRNA abundance of GPR109A (p ≤ 0.05), and a trend (p = 0.07) toward an increase was observed following treatment with 10 µM NA after 24 h (Figure 3). In contrast, treatment with NA had no effect on the mRNA abundances of AdipoR1/R2 or FABP4 (Table 1).


Nicotinic acid increases adiponectin secretion from differentiated bovine preadipocytes through G-protein coupled receptor signaling.

Kopp C, Hosseini A, Singh SP, Regenhard P, Khalilvandi-Behroozyar H, Sauerwein H, Mielenz M - Int J Mol Sci (2014)

The effects of nicotinic acid (NA) on the mRNA abundance of G-protein coupled receptor 109A (GPR109A) in differentiated bovine adipocytes cells (n = 10). After 4 h of starvation, the adipocytes were pre-incubated with (PTX (+)) or without pertussis toxin (PTX (−)) (100 ng/mL) for 16 h and then treated for 12 or 24 h with 10 or 15 µM NA or PBS (control). Due to the absence of differences, the PTX (+) and PTX (−) groups were merged for the analyses of the mRNA abundances. Significant differences (p ≤ 0.05) and trends (p ≤ 0.1) are designated with the corresponding p-values.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264232&req=5

ijms-15-21401-f003: The effects of nicotinic acid (NA) on the mRNA abundance of G-protein coupled receptor 109A (GPR109A) in differentiated bovine adipocytes cells (n = 10). After 4 h of starvation, the adipocytes were pre-incubated with (PTX (+)) or without pertussis toxin (PTX (−)) (100 ng/mL) for 16 h and then treated for 12 or 24 h with 10 or 15 µM NA or PBS (control). Due to the absence of differences, the PTX (+) and PTX (−) groups were merged for the analyses of the mRNA abundances. Significant differences (p ≤ 0.05) and trends (p ≤ 0.1) are designated with the corresponding p-values.
Mentions: Pre-incubation with PTX had no effect on the mRNA abundances of AdipoR1/2, FABP4, or GPR109A. Therefore, the PTX (+) and PTX (−) groups were merged for further analyses. Compared to the controls, incubation for 24 h with 15 µM NA increased the mRNA abundance of GPR109A (p ≤ 0.05), and a trend (p = 0.07) toward an increase was observed following treatment with 10 µM NA after 24 h (Figure 3). In contrast, treatment with NA had no effect on the mRNA abundances of AdipoR1/R2 or FABP4 (Table 1).

Bottom Line: Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle.Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h.The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.

View Article: PubMed Central - PubMed

Affiliation: Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany. christina.kopp@uni-bonn.de.

ABSTRACT
The transition period in dairy cows (3 weeks prepartum until 3 weeks postpartum) is associated with substantial mobilization of energy stores, which is often associated with metabolic diseases. Nicotinic acid (NA) is an antilipolytic and lipid-lowering compound used to treat dyslipidaemia in humans, and it also reduces non-esterified fatty acids in cattle. In mice the G-protein coupled receptor 109A (GPR109A) ligand NA positively affects the secretion of adiponectin, an important modulator of glucose and fat metabolism. In cattle, the corresponding data linking NA to adiponectin are missing. Our objective was to examine the effects of NA on adiponectin and AMPK protein abundance and the expression of mRNAs of related genes such as chemerin, an adipokine that enhances adiponectin secretion in vitro. Differentiated bovine adipocytes were incubated with pertussis toxin (PTX) to verify the involvement of GPR signaling, and treated with 10 or 15 µM NA for 12 or 24 h. NA increased adiponectin concentrations (p ≤ 0.001) and the mRNA abundances of GPR109A (p ≤ 0.05) and chemerin (p ≤ 0.01). Pre-incubation with PTX reduced the adiponectin response to NA (p ≤ 0.001). The NA-stimulated secretion of adiponectin and the mRNA expression of chemerin in the bovine adipocytes were suggestive of GPR signaling-dependent improved insulin sensitivity and/or adipocyte metabolism in dairy cows.

Show MeSH
Related in: MedlinePlus