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Enhanced levels of interleukin-8 are associated with hepatitis B virus infection and resistance to interferon-alpha therapy.

Yang K, Guan SH, Zhang H, Pan Y, Wu YY, Wang AH, Sun BB - Int J Mol Sci (2014)

Bottom Line: The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment.It is suggested that IL-8 might play important roles in the pathogenesis of CHB.Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China. ykmedicine@163.com.

ABSTRACT
The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined by enzyme linked immunosorbent assay (ELISA), and fluorescence-based quantitative real-time PCR (RT-qPCR) was used to measure IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with CHB. IL-8 protein expression was detected in liver biopsy tissues by immunohistochemistry. In addition, the differences in serum IL-8 and PBMCs mRNA levels were also observed in patients with different anti-viral responses to IFN-α. Compared to normal controls, serum IL-8 protein and mRNA levels were increased in CHB patients, IL-8 levels were positively correlated with the severity of liver inflammation/fibrosis. Moreover, serum IL-8 protein and mRNA levels were positively correlated with serum alanine aminotransferase (ALT) level and negatively correlated with serum prealbumin (PA) level. IL-8 expression was mainly located in portal area of liver tissues and was increased with the severity of liver inflammation and fibrosis stage. The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment. It is suggested that IL-8 might play important roles in the pathogenesis of CHB. Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment.

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The correlation between prealbumin (PA) levels and IL-8 expression. (A) Serum IL-8 protein was negatively correlated with serum PA; (B) PBMCs IL-8 mRNA was also negatively correlated with serum PA levels.
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ijms-15-21286-f005: The correlation between prealbumin (PA) levels and IL-8 expression. (A) Serum IL-8 protein was negatively correlated with serum PA; (B) PBMCs IL-8 mRNA was also negatively correlated with serum PA levels.

Mentions: An altered liver function may impair prealbumin (PA) synthesis. Clinical analysis revealed that serum PA level gradually decreased with the increase of hepatic IL-8 expression. Serum IL-8 expression was inversely proportional to serum PA level (r = 0.384, p = 0.001) (Figure 5A). In addition, IL-8 mRNA in PBMCs was also negatively correlated with serum PA level (r = 0.355, p = 0.003) (Figure 5B).


Enhanced levels of interleukin-8 are associated with hepatitis B virus infection and resistance to interferon-alpha therapy.

Yang K, Guan SH, Zhang H, Pan Y, Wu YY, Wang AH, Sun BB - Int J Mol Sci (2014)

The correlation between prealbumin (PA) levels and IL-8 expression. (A) Serum IL-8 protein was negatively correlated with serum PA; (B) PBMCs IL-8 mRNA was also negatively correlated with serum PA levels.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264225&req=5

ijms-15-21286-f005: The correlation between prealbumin (PA) levels and IL-8 expression. (A) Serum IL-8 protein was negatively correlated with serum PA; (B) PBMCs IL-8 mRNA was also negatively correlated with serum PA levels.
Mentions: An altered liver function may impair prealbumin (PA) synthesis. Clinical analysis revealed that serum PA level gradually decreased with the increase of hepatic IL-8 expression. Serum IL-8 expression was inversely proportional to serum PA level (r = 0.384, p = 0.001) (Figure 5A). In addition, IL-8 mRNA in PBMCs was also negatively correlated with serum PA level (r = 0.355, p = 0.003) (Figure 5B).

Bottom Line: The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment.It is suggested that IL-8 might play important roles in the pathogenesis of CHB.Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China. ykmedicine@163.com.

ABSTRACT
The objective of this study was to analyze the expression levels of IL-8 in serum and liver tissues from patients with chronic hepatitis B (CHB) infection and to investigate whether IL-8 may antagonize interferon-alpha (IFN-α) antiviral activity against HBV. IL-8 expression in serum was determined by enzyme linked immunosorbent assay (ELISA), and fluorescence-based quantitative real-time PCR (RT-qPCR) was used to measure IL-8 mRNA in peripheral blood mononuclear cells (PBMCs) in patients with CHB. IL-8 protein expression was detected in liver biopsy tissues by immunohistochemistry. In addition, the differences in serum IL-8 and PBMCs mRNA levels were also observed in patients with different anti-viral responses to IFN-α. Compared to normal controls, serum IL-8 protein and mRNA levels were increased in CHB patients, IL-8 levels were positively correlated with the severity of liver inflammation/fibrosis. Moreover, serum IL-8 protein and mRNA levels were positively correlated with serum alanine aminotransferase (ALT) level and negatively correlated with serum prealbumin (PA) level. IL-8 expression was mainly located in portal area of liver tissues and was increased with the severity of liver inflammation and fibrosis stage. The expression serum and mRNA levels of IL-8 in the CHB patients with a complete response to IFN-α are significantly lower than that of the patients with non-response to IFN-α treatment. It is suggested that IL-8 might play important roles in the pathogenesis of CHB. Moreover, interferon resistance may be related to the up-regulation of IL-8 expression in the patients did not respond to IFN-α treatment.

Show MeSH
Related in: MedlinePlus