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Nickel nanoparticles exposure and reproductive toxicity in healthy adult rats.

Kong L, Tang M, Zhang T, Wang D, Hu K, Lu W, Wei C, Liang G, Pu Y - Int J Mol Sci (2014)

Bottom Line: Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups.Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group.Further research is needed to elucidate exposure to human populations and mechanism of actions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. konglu@seu.edu.cn.

ABSTRACT
Nickel is associated with reproductive toxicity. However, the reproductive toxicity of nickel nanoparticles (Ni NPs) is unclear. Our goal was to determine the association between nickel nanoparticle exposure and reproductive toxicity. According to the one-generation reproductive toxicity standard, rats were exposed to nickel nanoparticles by gavage and we selected indicators including sex hormone levels, sperm motility, histopathology, and reproductive outcome etc. Experimental results showed nickel nanoparticles increased follicle stimulating hormone (FSH) and luteinizing hormone (LH), and lowered etradiol (E2) serum levels at a dose of 15 and 45 mg/kg in female rats. Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups. For male rats, the weights decreased gradually, the ratio of epididymis weight over body weight increased, the motility of rat sperm changed, and the levels of FSH and testosterone (T) diminished. Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group. At the same time, Ni NPs resulted in a change of the reproductive index and the offspring development of rats. Further research is needed to elucidate exposure to human populations and mechanism of actions.

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Effects of Ni NPs on serum hormone concentrations in rats. Follicle stimulating hormone (FSH) in females (A); Luteinizing hormone (LH) in females (B); Estradiol (E2) in females (C); FSH in males (D); LH in males (E); Testosterone (T) in males (F). Serum hormone concentrations were measured by ELISA. Values represent the mean ± SD (n = 7). * p < 0.05, compared with control group (0 mg/kg BW); 1p < 0.05, compared with Ni MPs (45 mg/kg BW).
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ijms-15-21253-f002: Effects of Ni NPs on serum hormone concentrations in rats. Follicle stimulating hormone (FSH) in females (A); Luteinizing hormone (LH) in females (B); Estradiol (E2) in females (C); FSH in males (D); LH in males (E); Testosterone (T) in males (F). Serum hormone concentrations were measured by ELISA. Values represent the mean ± SD (n = 7). * p < 0.05, compared with control group (0 mg/kg BW); 1p < 0.05, compared with Ni MPs (45 mg/kg BW).

Mentions: To determine whether Ni NPs exposure induces alterations to the female or male reproductive system, according to the one-generation reproductive toxicity study, we treated adult female Sprague-Dawley rats with Ni NPs at 5 mg/kg BW (low dose), 15 mg/kg BW (mid-dose), and 45 mg/kg BW (high dose) for eighteen weeks by gavage. Similarly we treated male rats with Ni NPs for ten weeks. Mid-dose and high dose of Ni NPs significantly increased serum FSH concentrations in female rats compared with controls (Figure 2A), and all doses of Ni NPs significantly increased LH in female rats (Figure 2B). However, high dose of Ni NPs significantly increased serum FSH and LH concentrations compared to Ni MPs (Figure 2A,B). In contrast, the serum E2 of the females was decreased by Ni NPs exposure (Figure 2C). Exposure to Ni NPs (mid-dose and high-dose) resulted in the same alteration of serum FSH and T concentrations in male rats as observed with Ni MPs (Figure 2D,F). Compared with Ni MPs, the levels of FSH and T in serum were significantly lower while the level of LH was significantly higher in the high dose of Ni NPs (Figure 2D–F).


Nickel nanoparticles exposure and reproductive toxicity in healthy adult rats.

Kong L, Tang M, Zhang T, Wang D, Hu K, Lu W, Wei C, Liang G, Pu Y - Int J Mol Sci (2014)

Effects of Ni NPs on serum hormone concentrations in rats. Follicle stimulating hormone (FSH) in females (A); Luteinizing hormone (LH) in females (B); Estradiol (E2) in females (C); FSH in males (D); LH in males (E); Testosterone (T) in males (F). Serum hormone concentrations were measured by ELISA. Values represent the mean ± SD (n = 7). * p < 0.05, compared with control group (0 mg/kg BW); 1p < 0.05, compared with Ni MPs (45 mg/kg BW).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264223&req=5

ijms-15-21253-f002: Effects of Ni NPs on serum hormone concentrations in rats. Follicle stimulating hormone (FSH) in females (A); Luteinizing hormone (LH) in females (B); Estradiol (E2) in females (C); FSH in males (D); LH in males (E); Testosterone (T) in males (F). Serum hormone concentrations were measured by ELISA. Values represent the mean ± SD (n = 7). * p < 0.05, compared with control group (0 mg/kg BW); 1p < 0.05, compared with Ni MPs (45 mg/kg BW).
Mentions: To determine whether Ni NPs exposure induces alterations to the female or male reproductive system, according to the one-generation reproductive toxicity study, we treated adult female Sprague-Dawley rats with Ni NPs at 5 mg/kg BW (low dose), 15 mg/kg BW (mid-dose), and 45 mg/kg BW (high dose) for eighteen weeks by gavage. Similarly we treated male rats with Ni NPs for ten weeks. Mid-dose and high dose of Ni NPs significantly increased serum FSH concentrations in female rats compared with controls (Figure 2A), and all doses of Ni NPs significantly increased LH in female rats (Figure 2B). However, high dose of Ni NPs significantly increased serum FSH and LH concentrations compared to Ni MPs (Figure 2A,B). In contrast, the serum E2 of the females was decreased by Ni NPs exposure (Figure 2C). Exposure to Ni NPs (mid-dose and high-dose) resulted in the same alteration of serum FSH and T concentrations in male rats as observed with Ni MPs (Figure 2D,F). Compared with Ni MPs, the levels of FSH and T in serum were significantly lower while the level of LH was significantly higher in the high dose of Ni NPs (Figure 2D–F).

Bottom Line: Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups.Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group.Further research is needed to elucidate exposure to human populations and mechanism of actions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. konglu@seu.edu.cn.

ABSTRACT
Nickel is associated with reproductive toxicity. However, the reproductive toxicity of nickel nanoparticles (Ni NPs) is unclear. Our goal was to determine the association between nickel nanoparticle exposure and reproductive toxicity. According to the one-generation reproductive toxicity standard, rats were exposed to nickel nanoparticles by gavage and we selected indicators including sex hormone levels, sperm motility, histopathology, and reproductive outcome etc. Experimental results showed nickel nanoparticles increased follicle stimulating hormone (FSH) and luteinizing hormone (LH), and lowered etradiol (E2) serum levels at a dose of 15 and 45 mg/kg in female rats. Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups. For male rats, the weights decreased gradually, the ratio of epididymis weight over body weight increased, the motility of rat sperm changed, and the levels of FSH and testosterone (T) diminished. Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group. At the same time, Ni NPs resulted in a change of the reproductive index and the offspring development of rats. Further research is needed to elucidate exposure to human populations and mechanism of actions.

Show MeSH
Related in: MedlinePlus