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Nickel nanoparticles exposure and reproductive toxicity in healthy adult rats.

Kong L, Tang M, Zhang T, Wang D, Hu K, Lu W, Wei C, Liang G, Pu Y - Int J Mol Sci (2014)

Bottom Line: Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups.Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group.Further research is needed to elucidate exposure to human populations and mechanism of actions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. konglu@seu.edu.cn.

ABSTRACT
Nickel is associated with reproductive toxicity. However, the reproductive toxicity of nickel nanoparticles (Ni NPs) is unclear. Our goal was to determine the association between nickel nanoparticle exposure and reproductive toxicity. According to the one-generation reproductive toxicity standard, rats were exposed to nickel nanoparticles by gavage and we selected indicators including sex hormone levels, sperm motility, histopathology, and reproductive outcome etc. Experimental results showed nickel nanoparticles increased follicle stimulating hormone (FSH) and luteinizing hormone (LH), and lowered etradiol (E2) serum levels at a dose of 15 and 45 mg/kg in female rats. Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups. For male rats, the weights decreased gradually, the ratio of epididymis weight over body weight increased, the motility of rat sperm changed, and the levels of FSH and testosterone (T) diminished. Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group. At the same time, Ni NPs resulted in a change of the reproductive index and the offspring development of rats. Further research is needed to elucidate exposure to human populations and mechanism of actions.

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SEM (scale bar = 200 nm) (a) and TEM (scale bar = 100 nm) (b) images and particle size distributions of nickel nanoparticles (Ni NPs) of 5 μg/mL (c) and 12.5 μg/mL (d).
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ijms-15-21253-f001: SEM (scale bar = 200 nm) (a) and TEM (scale bar = 100 nm) (b) images and particle size distributions of nickel nanoparticles (Ni NPs) of 5 μg/mL (c) and 12.5 μg/mL (d).

Mentions: The SEM and TEM images showed that Ni NPs were spherical in shape. The size distribution varied from 30 to 100 nm in diameter, the average size of Ni NPs was 90 nm, and there was slight agglomeration. In the dispersion, the particle size of Ni NPs of 5 μg/mL had a distribution from 260 to 725 nm, and peak size was about 444 nm. However, the average particle size of 12.5 μg/mL had a distribution from 400 to 879 nm, and peak size of about 522 nm. See Figure 1. Meanwhile the average size distribution of Ni MPs is 3.34 ± 0.67 µm without agglomeration.


Nickel nanoparticles exposure and reproductive toxicity in healthy adult rats.

Kong L, Tang M, Zhang T, Wang D, Hu K, Lu W, Wei C, Liang G, Pu Y - Int J Mol Sci (2014)

SEM (scale bar = 200 nm) (a) and TEM (scale bar = 100 nm) (b) images and particle size distributions of nickel nanoparticles (Ni NPs) of 5 μg/mL (c) and 12.5 μg/mL (d).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264223&req=5

ijms-15-21253-f001: SEM (scale bar = 200 nm) (a) and TEM (scale bar = 100 nm) (b) images and particle size distributions of nickel nanoparticles (Ni NPs) of 5 μg/mL (c) and 12.5 μg/mL (d).
Mentions: The SEM and TEM images showed that Ni NPs were spherical in shape. The size distribution varied from 30 to 100 nm in diameter, the average size of Ni NPs was 90 nm, and there was slight agglomeration. In the dispersion, the particle size of Ni NPs of 5 μg/mL had a distribution from 260 to 725 nm, and peak size was about 444 nm. However, the average particle size of 12.5 μg/mL had a distribution from 400 to 879 nm, and peak size of about 522 nm. See Figure 1. Meanwhile the average size distribution of Ni MPs is 3.34 ± 0.67 µm without agglomeration.

Bottom Line: Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups.Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group.Further research is needed to elucidate exposure to human populations and mechanism of actions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China. konglu@seu.edu.cn.

ABSTRACT
Nickel is associated with reproductive toxicity. However, the reproductive toxicity of nickel nanoparticles (Ni NPs) is unclear. Our goal was to determine the association between nickel nanoparticle exposure and reproductive toxicity. According to the one-generation reproductive toxicity standard, rats were exposed to nickel nanoparticles by gavage and we selected indicators including sex hormone levels, sperm motility, histopathology, and reproductive outcome etc. Experimental results showed nickel nanoparticles increased follicle stimulating hormone (FSH) and luteinizing hormone (LH), and lowered etradiol (E2) serum levels at a dose of 15 and 45 mg/kg in female rats. Ovarian lymphocytosis, vascular dilatation and congestion, inflammatory cell infiltration, and increase in apoptotic cells were found in ovary tissues in exposure groups. For male rats, the weights decreased gradually, the ratio of epididymis weight over body weight increased, the motility of rat sperm changed, and the levels of FSH and testosterone (T) diminished. Pathological results showed the shedding of epithelial cells of raw seminiferous tubule, disordered arrangement of cells in the tube, and the appearance of cell apoptosis and death in the exposure group. At the same time, Ni NPs resulted in a change of the reproductive index and the offspring development of rats. Further research is needed to elucidate exposure to human populations and mechanism of actions.

Show MeSH
Related in: MedlinePlus