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Molecular characterization of the BMP7 gene and its potential role in shell formation in Pinctada martensii.

Yan F, Luo S, Jiao Y, Deng Y, Du X, Huang R, Wang Q, Chen W - Int J Mol Sci (2014)

Bottom Line: After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue).The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group.These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell.

View Article: PubMed Central - PubMed

Affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, Guangdong, China. yanfanghdxs@163.com.

ABSTRACT
Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encoding a 429 amino acid polypeptide and a 3'-UTR of 1388 bp. The deduced protein sequence of Pm-BMP7 contained a signal peptide, a pro-domain and a mature peptide. The mature peptide consisted of 135 amino acids and included a transforming growth factor β family domain with six shared cysteine residues. The protein sequence of Pm-BMP7 showed 66% identity with that from Crassostrea gigas. Two unigenes encoding Pm-BMPRI (Pm-BMP receptor I) and Pm-BMPRII were obtained from the transcriptome database of P. martensii. Tissue expression analysis demonstrated Pm-BMP7 and Pm-BMPRI were highly expressed in the mantle (shell formation related-tissue), while Pm-BMPRII was highly expressed in the foot. After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue). The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group. These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell.

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Related in: MedlinePlus

Expression pattern of Pm-BMP7 and its receptors mRNA in different tissues by qRT-PCR. Each bar is a mean of the different tissues (adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot) from the five pearl oysters. The pearl oyster β-actin gene is used as the reference gene. The significant difference is indicated by different letters (p < 0.05). The error bars correspond to the mean + standard error (SE).
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ijms-15-21215-f004: Expression pattern of Pm-BMP7 and its receptors mRNA in different tissues by qRT-PCR. Each bar is a mean of the different tissues (adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot) from the five pearl oysters. The pearl oyster β-actin gene is used as the reference gene. The significant difference is indicated by different letters (p < 0.05). The error bars correspond to the mean + standard error (SE).

Mentions: Studies have shown that BMP7 regulates cell proliferation and differentiation by signaling through two structurally related type I (BMPRI) and type II receptors (BMPRII). Two unigenes encoding Pm-BMPRI and Pm-BMPRII were obtained in the transcriptome database of P. martensii. The unigene sequences can be found in Figure S1. The qRT-PCR was performed to determine the tissue specific expression of Pm-BMP7 and its receptors in the adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot with β-actin as the internal reference. The Pm-BMP7 mRNA was highly expressed in the mantle, which is shell formation related-tissue (Figure 4). Similarly to the Pm-BMP7 expression profile, Pm-BMPRI mRNA was highly expressed in the mantle, while Pm-BMPRII was highly expressed in the foot.


Molecular characterization of the BMP7 gene and its potential role in shell formation in Pinctada martensii.

Yan F, Luo S, Jiao Y, Deng Y, Du X, Huang R, Wang Q, Chen W - Int J Mol Sci (2014)

Expression pattern of Pm-BMP7 and its receptors mRNA in different tissues by qRT-PCR. Each bar is a mean of the different tissues (adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot) from the five pearl oysters. The pearl oyster β-actin gene is used as the reference gene. The significant difference is indicated by different letters (p < 0.05). The error bars correspond to the mean + standard error (SE).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264221&req=5

ijms-15-21215-f004: Expression pattern of Pm-BMP7 and its receptors mRNA in different tissues by qRT-PCR. Each bar is a mean of the different tissues (adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot) from the five pearl oysters. The pearl oyster β-actin gene is used as the reference gene. The significant difference is indicated by different letters (p < 0.05). The error bars correspond to the mean + standard error (SE).
Mentions: Studies have shown that BMP7 regulates cell proliferation and differentiation by signaling through two structurally related type I (BMPRI) and type II receptors (BMPRII). Two unigenes encoding Pm-BMPRI and Pm-BMPRII were obtained in the transcriptome database of P. martensii. The unigene sequences can be found in Figure S1. The qRT-PCR was performed to determine the tissue specific expression of Pm-BMP7 and its receptors in the adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad and foot with β-actin as the internal reference. The Pm-BMP7 mRNA was highly expressed in the mantle, which is shell formation related-tissue (Figure 4). Similarly to the Pm-BMP7 expression profile, Pm-BMPRI mRNA was highly expressed in the mantle, while Pm-BMPRII was highly expressed in the foot.

Bottom Line: After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue).The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group.These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell.

View Article: PubMed Central - PubMed

Affiliation: Fishery College, Guangdong Ocean University, 40 East Jiefang Road, Xiashan District, Zhanjiang 524025, Guangdong, China. yanfanghdxs@163.com.

ABSTRACT
Bone morphogenetic protein 7 (BMP7), also called osteogenetic protein-1, can induce bone formation. In this study, the obtained full-length cDNA of BMP7 from Pinctada martensii (Pm-BMP7) was 2972 bp, including a 5'-untranslated region (UTR) of 294 bp, an open reading fragment of 1290 bp encoding a 429 amino acid polypeptide and a 3'-UTR of 1388 bp. The deduced protein sequence of Pm-BMP7 contained a signal peptide, a pro-domain and a mature peptide. The mature peptide consisted of 135 amino acids and included a transforming growth factor β family domain with six shared cysteine residues. The protein sequence of Pm-BMP7 showed 66% identity with that from Crassostrea gigas. Two unigenes encoding Pm-BMPRI (Pm-BMP receptor I) and Pm-BMPRII were obtained from the transcriptome database of P. martensii. Tissue expression analysis demonstrated Pm-BMP7 and Pm-BMPRI were highly expressed in the mantle (shell formation related-tissue), while Pm-BMPRII was highly expressed in the foot. After inhibiting Pm-BMP7 expression using RNA interference (RNAi) technology, Pm-BMP7 mRNA was significantly down-regulated (p < 0.05) in the mantle pallium (nacre formation related-tissue) and the mantle edge (prismatic layer formation related-tissue). The microstructure, observed using a scanning electron microscope, indicated a disordered growth status in the nacre and obvious holes in the prismatic layer in the dsRNA-Pm-BMP7 injected-group. These results suggest that Pm-BMP7 plays a crucial role in the nacre and prismatic layer formation process of the shell.

Show MeSH
Related in: MedlinePlus