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Isolation and multiple differentiation potential assessment of human gingival mesenchymal stem cells.

Gao Y, Zhao G, Li D, Chen X, Pang J, Ke J - Int J Mol Sci (2014)

Bottom Line: GMSCs showed the medial osteogenic and adipogenic differentiation potentials between those of PDLSCs and DSCs.GMSCs were capable of expressing the odontogenic genes after ETGC-CM induction.This study provides evidence that GMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.

View Article: PubMed Central - PubMed

Affiliation: Department of Stomatology, Air Force General Hospital, People's Liberation Army (PLA), Beijing 100142, China. lansefengqing1981@hotmail.com.

ABSTRACT
The aim of this study was to isolate human mesenchymal stem cells (MSCs) from the gingiva (GMSCs) and confirm their multiple differentiation potentials, including the odontogenic lineage. GMSCs, periodontal ligament stem cells (PDLSCs) and dermal stem cells (DSCs) cultures were analyzed for cell shape, cell cycle, colony-forming unit-fibroblast (CFU-F) and stem cell markers. Cells were then induced for osteogenic and adipogenic differentiation and analyzed for differentiation markers (alkaline phosphatase (ALP) activity, mineralization nodule formation and Runx2, ALP, osteocalcin (OCN) and collagen I expressions for the osteogenic differentiation, and lipid vacuole formation and PPARĪ³-2 expression for the adipogenic differentiation). Besides, the odontogenic differentiation potential of GMSCs induced with embryonic tooth germ cell-conditioned medium (ETGC-CM) was observed. GMSCs, PDLSCs and DSCs were all stromal origin. PDLSCs showed much higher osteogenic differentiation ability but lower adipogenic differentiation potential than DSCs. GMSCs showed the medial osteogenic and adipogenic differentiation potentials between those of PDLSCs and DSCs. GMSCs were capable of expressing the odontogenic genes after ETGC-CM induction. This study provides evidence that GMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.

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Morphology of the primary mesenchymal stem cells (MSCs) established from human gingiva, periodontal ligament and dermis (P3).
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ijms-15-20982-f001: Morphology of the primary mesenchymal stem cells (MSCs) established from human gingiva, periodontal ligament and dermis (P3).

Mentions: No significant differences were observed in the morphological characteristics of GMSCs, PDLSCs and DSCs (Figure 1). All the cells assumed a fibroblast-like spindle cell shape (Figure 1). The cell cycle propagation of MSCs was also monitored and the results are shown in Figure 2. GMSCs had more cells in the percentage of S + G2M phases (48.4%), compared to PDLSCs (19.46%), but lower when compared to DSCs (84.9%).


Isolation and multiple differentiation potential assessment of human gingival mesenchymal stem cells.

Gao Y, Zhao G, Li D, Chen X, Pang J, Ke J - Int J Mol Sci (2014)

Morphology of the primary mesenchymal stem cells (MSCs) established from human gingiva, periodontal ligament and dermis (P3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4264207&req=5

ijms-15-20982-f001: Morphology of the primary mesenchymal stem cells (MSCs) established from human gingiva, periodontal ligament and dermis (P3).
Mentions: No significant differences were observed in the morphological characteristics of GMSCs, PDLSCs and DSCs (Figure 1). All the cells assumed a fibroblast-like spindle cell shape (Figure 1). The cell cycle propagation of MSCs was also monitored and the results are shown in Figure 2. GMSCs had more cells in the percentage of S + G2M phases (48.4%), compared to PDLSCs (19.46%), but lower when compared to DSCs (84.9%).

Bottom Line: GMSCs showed the medial osteogenic and adipogenic differentiation potentials between those of PDLSCs and DSCs.GMSCs were capable of expressing the odontogenic genes after ETGC-CM induction.This study provides evidence that GMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.

View Article: PubMed Central - PubMed

Affiliation: Department of Stomatology, Air Force General Hospital, People's Liberation Army (PLA), Beijing 100142, China. lansefengqing1981@hotmail.com.

ABSTRACT
The aim of this study was to isolate human mesenchymal stem cells (MSCs) from the gingiva (GMSCs) and confirm their multiple differentiation potentials, including the odontogenic lineage. GMSCs, periodontal ligament stem cells (PDLSCs) and dermal stem cells (DSCs) cultures were analyzed for cell shape, cell cycle, colony-forming unit-fibroblast (CFU-F) and stem cell markers. Cells were then induced for osteogenic and adipogenic differentiation and analyzed for differentiation markers (alkaline phosphatase (ALP) activity, mineralization nodule formation and Runx2, ALP, osteocalcin (OCN) and collagen I expressions for the osteogenic differentiation, and lipid vacuole formation and PPARĪ³-2 expression for the adipogenic differentiation). Besides, the odontogenic differentiation potential of GMSCs induced with embryonic tooth germ cell-conditioned medium (ETGC-CM) was observed. GMSCs, PDLSCs and DSCs were all stromal origin. PDLSCs showed much higher osteogenic differentiation ability but lower adipogenic differentiation potential than DSCs. GMSCs showed the medial osteogenic and adipogenic differentiation potentials between those of PDLSCs and DSCs. GMSCs were capable of expressing the odontogenic genes after ETGC-CM induction. This study provides evidence that GMSCs can be used in tissue engineering/regeneration protocols as an approachable stem cell source.

Show MeSH
Related in: MedlinePlus