Fast and efficient neural conversion of human hematopoietic cells.
Bottom Line: SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs.However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation.Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency.
Affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Barcelona 08036, Spain.Show MeSH
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Mentions: A more extensive quantitative RT-PCR (qRT-PCR) analysis of CB-iNCs confirmed a remarkable upregulation of TUJ1, doublecortin (DCX), and MAP2 already at 15 days after neural induction, whereas PAX6 and early neural markers (MUSASHI, SOX1, and NESTIN) were present at lower levels (Figure 2A). While recent work identified MASH1 as the most important driver of neural conversion in fibroblasts (Chanda et al., 2014), we observed only marginal induction of MASH1 expression in CB-iNCs (Figure 2A). In contrast, BRN2 was progressively activated in CB-iNCs during the neural conversion (Figure 2A), suggesting an involvement of BRN2 in this process, in line with other recent reports (Lujan et al., 2012; Zou et al., 2014).
Affiliation: Josep Carreras Leukemia Research Institute, Cell Therapy Program of the University of Barcelona, Barcelona 08036, Spain.