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Resveratrol possesses protective effects in a pristane-induced lupus mouse model.

Wang ZL, Luo XF, Li MT, Xu D, Zhou S, Chen HZ, Gao N, Chen Z, Zhang LL, Zeng XF - PLoS ONE (2014)

Bottom Line: To date, no therapy has been found to satisfactorily treat SLE.Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro.In vitro antibody production and proliferation of B cells were also inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by the production of autoantibodies. To date, no therapy has been found to satisfactorily treat SLE. SIRT1 deficiency results in the development of an autoimmune syndrome in mice, including a high titer of anti-nuclear antibody in serum, immunoglobulin deposition in the kidney, and immune complex glomerulonephritis. Resveratrol is an activator of SIRT1 and possesses anti-inflammation and immune-regulatory properties.

Objective: To evaluate the preventative effects of resveratrol on a pristane-induced lupus animal model and assess its putative immune modulation effects.

Methods: BALB/c mice received a single intraperitoneal injection of 0.5 ml of pristane on day 1 and then various doses of resveratrol were given to the mice daily starting on day 2 and continuing for seven months. The autoantibodies in serum and supernatants were measured. Single cells isolated from spleen, isolated CD4+ T cells, and CD19+ B cells were cultured with or without resveratrol in vitro and assessed by flow cytometry.

Results: Resveratrol attenuated proteinuria, immunoglobuin depositon in kidney, and glomerulonephritis as well as IgG1 and IgG2a in serum in pristane-induced lupus mice. Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro. In vitro antibody production and proliferation of B cells were also inhibited.

Conclusion: Resveratrol possesses protective effects in pristane-induced lupus mice and may represent a novel approach for the management of SLE.

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Related in: MedlinePlus

Effects of resveratrol on B lymphocytes in vitro.(A): Resveratrol inhibits B lymphocyte proliferation [mean ± standard deviation (SD); n = 8]. (B) Resveratrol decreases antibody production of B lymphocytes. (mean ± SD; n = 3) (★: P<0.05; ★★: <0.01; ★★★: <0.001).
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pone-0114792-g004: Effects of resveratrol on B lymphocytes in vitro.(A): Resveratrol inhibits B lymphocyte proliferation [mean ± standard deviation (SD); n = 8]. (B) Resveratrol decreases antibody production of B lymphocytes. (mean ± SD; n = 3) (★: P<0.05; ★★: <0.01; ★★★: <0.001).

Mentions: Freshly islated CD19+ B lymphocytes were cultured with LPS (10 µg/ml) and increasing concentrations of resveratrol (0, 10, 20, or 40 µM, respectively) for 4 d in vitro and then assessed for proliferation. Resveratrol significantly inhibited B cell proliferation (F = 49.56; p<0.0001) compared to the controls in a concentration-dependent manner (Fig. 4A). To investigate the potential effect of resveratrol on B cells, purified B cells were cultured with LPS (10 µg/ml) with or without resveratrol (0, 20, 40, or 80 µM, respectively) for 7 d in vitro, and then Ig isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) were measured in the supernatant. Compared to the controls, resveratrol significantly decreased the production of IgG1 (F = 10.923; P = 0.003), IgG2a (F = 16.001; P = 0.001), IgG2b (F = 16.934; P = 0.001), IgG3 (F = 20.167; p<0.0001), IgM (F = 33.541; p<0.0001), and IgA (F = 14.495; P = 0.001) (Fig. 4B).


Resveratrol possesses protective effects in a pristane-induced lupus mouse model.

Wang ZL, Luo XF, Li MT, Xu D, Zhou S, Chen HZ, Gao N, Chen Z, Zhang LL, Zeng XF - PLoS ONE (2014)

Effects of resveratrol on B lymphocytes in vitro.(A): Resveratrol inhibits B lymphocyte proliferation [mean ± standard deviation (SD); n = 8]. (B) Resveratrol decreases antibody production of B lymphocytes. (mean ± SD; n = 3) (★: P<0.05; ★★: <0.01; ★★★: <0.001).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263676&req=5

pone-0114792-g004: Effects of resveratrol on B lymphocytes in vitro.(A): Resveratrol inhibits B lymphocyte proliferation [mean ± standard deviation (SD); n = 8]. (B) Resveratrol decreases antibody production of B lymphocytes. (mean ± SD; n = 3) (★: P<0.05; ★★: <0.01; ★★★: <0.001).
Mentions: Freshly islated CD19+ B lymphocytes were cultured with LPS (10 µg/ml) and increasing concentrations of resveratrol (0, 10, 20, or 40 µM, respectively) for 4 d in vitro and then assessed for proliferation. Resveratrol significantly inhibited B cell proliferation (F = 49.56; p<0.0001) compared to the controls in a concentration-dependent manner (Fig. 4A). To investigate the potential effect of resveratrol on B cells, purified B cells were cultured with LPS (10 µg/ml) with or without resveratrol (0, 20, 40, or 80 µM, respectively) for 7 d in vitro, and then Ig isotypes (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) were measured in the supernatant. Compared to the controls, resveratrol significantly decreased the production of IgG1 (F = 10.923; P = 0.003), IgG2a (F = 16.001; P = 0.001), IgG2b (F = 16.934; P = 0.001), IgG3 (F = 20.167; p<0.0001), IgM (F = 33.541; p<0.0001), and IgA (F = 14.495; P = 0.001) (Fig. 4B).

Bottom Line: To date, no therapy has been found to satisfactorily treat SLE.Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro.In vitro antibody production and proliferation of B cells were also inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by the production of autoantibodies. To date, no therapy has been found to satisfactorily treat SLE. SIRT1 deficiency results in the development of an autoimmune syndrome in mice, including a high titer of anti-nuclear antibody in serum, immunoglobulin deposition in the kidney, and immune complex glomerulonephritis. Resveratrol is an activator of SIRT1 and possesses anti-inflammation and immune-regulatory properties.

Objective: To evaluate the preventative effects of resveratrol on a pristane-induced lupus animal model and assess its putative immune modulation effects.

Methods: BALB/c mice received a single intraperitoneal injection of 0.5 ml of pristane on day 1 and then various doses of resveratrol were given to the mice daily starting on day 2 and continuing for seven months. The autoantibodies in serum and supernatants were measured. Single cells isolated from spleen, isolated CD4+ T cells, and CD19+ B cells were cultured with or without resveratrol in vitro and assessed by flow cytometry.

Results: Resveratrol attenuated proteinuria, immunoglobuin depositon in kidney, and glomerulonephritis as well as IgG1 and IgG2a in serum in pristane-induced lupus mice. Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro. In vitro antibody production and proliferation of B cells were also inhibited.

Conclusion: Resveratrol possesses protective effects in pristane-induced lupus mice and may represent a novel approach for the management of SLE.

Show MeSH
Related in: MedlinePlus