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Resveratrol possesses protective effects in a pristane-induced lupus mouse model.

Wang ZL, Luo XF, Li MT, Xu D, Zhou S, Chen HZ, Gao N, Chen Z, Zhang LL, Zeng XF - PLoS ONE (2014)

Bottom Line: To date, no therapy has been found to satisfactorily treat SLE.Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro.In vitro antibody production and proliferation of B cells were also inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by the production of autoantibodies. To date, no therapy has been found to satisfactorily treat SLE. SIRT1 deficiency results in the development of an autoimmune syndrome in mice, including a high titer of anti-nuclear antibody in serum, immunoglobulin deposition in the kidney, and immune complex glomerulonephritis. Resveratrol is an activator of SIRT1 and possesses anti-inflammation and immune-regulatory properties.

Objective: To evaluate the preventative effects of resveratrol on a pristane-induced lupus animal model and assess its putative immune modulation effects.

Methods: BALB/c mice received a single intraperitoneal injection of 0.5 ml of pristane on day 1 and then various doses of resveratrol were given to the mice daily starting on day 2 and continuing for seven months. The autoantibodies in serum and supernatants were measured. Single cells isolated from spleen, isolated CD4+ T cells, and CD19+ B cells were cultured with or without resveratrol in vitro and assessed by flow cytometry.

Results: Resveratrol attenuated proteinuria, immunoglobuin depositon in kidney, and glomerulonephritis as well as IgG1 and IgG2a in serum in pristane-induced lupus mice. Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro. In vitro antibody production and proliferation of B cells were also inhibited.

Conclusion: Resveratrol possesses protective effects in pristane-induced lupus mice and may represent a novel approach for the management of SLE.

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Related in: MedlinePlus

Kidney pathology.The kidney sections were stained with hematoxylin and eosin (H&E) and representative images are shown. (A): Normal glomerulus from a normal control mouse (n = 10). (B): Proliferative glomerular lesions and mononuclear cell infiltration in glomerulus from a model control mouse (n  =  10). (C): A marked reduction in pathological lesions in glomerulus from a mouse from the resveratrol A group (n  =  10) (D): A marked reduction in pathological lesions is observed in glomerulus from a representative resveratrol B mouse (n  =  10). (original magnification×200)
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pone-0114792-g001: Kidney pathology.The kidney sections were stained with hematoxylin and eosin (H&E) and representative images are shown. (A): Normal glomerulus from a normal control mouse (n = 10). (B): Proliferative glomerular lesions and mononuclear cell infiltration in glomerulus from a model control mouse (n  =  10). (C): A marked reduction in pathological lesions in glomerulus from a mouse from the resveratrol A group (n  =  10) (D): A marked reduction in pathological lesions is observed in glomerulus from a representative resveratrol B mouse (n  =  10). (original magnification×200)

Mentions: At the end of observation period, light microscopy of kidney sections revealed that 6 mice developed segmental or diffuse proliferative glomerular lesions with mononuclear cell infiltration among 10 model control mice (Fig. 1B): 3 mice among 10 resveratrol A group mice and 10 resveratrol B group mice, respectively. No mouse developed glomerular lesions among the normal control group mice. The mice from the resveratrol A group had a marked reduction in pathological lesions of the glomerulus compared to mice of the model control group (Fig. 1C) and slightly less lymphocyte infiltration was also observed. Similar results were detected in mice from the resveratrol B group as well (Fig. 1D).


Resveratrol possesses protective effects in a pristane-induced lupus mouse model.

Wang ZL, Luo XF, Li MT, Xu D, Zhou S, Chen HZ, Gao N, Chen Z, Zhang LL, Zeng XF - PLoS ONE (2014)

Kidney pathology.The kidney sections were stained with hematoxylin and eosin (H&E) and representative images are shown. (A): Normal glomerulus from a normal control mouse (n = 10). (B): Proliferative glomerular lesions and mononuclear cell infiltration in glomerulus from a model control mouse (n  =  10). (C): A marked reduction in pathological lesions in glomerulus from a mouse from the resveratrol A group (n  =  10) (D): A marked reduction in pathological lesions is observed in glomerulus from a representative resveratrol B mouse (n  =  10). (original magnification×200)
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263676&req=5

pone-0114792-g001: Kidney pathology.The kidney sections were stained with hematoxylin and eosin (H&E) and representative images are shown. (A): Normal glomerulus from a normal control mouse (n = 10). (B): Proliferative glomerular lesions and mononuclear cell infiltration in glomerulus from a model control mouse (n  =  10). (C): A marked reduction in pathological lesions in glomerulus from a mouse from the resveratrol A group (n  =  10) (D): A marked reduction in pathological lesions is observed in glomerulus from a representative resveratrol B mouse (n  =  10). (original magnification×200)
Mentions: At the end of observation period, light microscopy of kidney sections revealed that 6 mice developed segmental or diffuse proliferative glomerular lesions with mononuclear cell infiltration among 10 model control mice (Fig. 1B): 3 mice among 10 resveratrol A group mice and 10 resveratrol B group mice, respectively. No mouse developed glomerular lesions among the normal control group mice. The mice from the resveratrol A group had a marked reduction in pathological lesions of the glomerulus compared to mice of the model control group (Fig. 1C) and slightly less lymphocyte infiltration was also observed. Similar results were detected in mice from the resveratrol B group as well (Fig. 1D).

Bottom Line: To date, no therapy has been found to satisfactorily treat SLE.Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro.In vitro antibody production and proliferation of B cells were also inhibited.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

ABSTRACT

Background: Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease characterized by the production of autoantibodies. To date, no therapy has been found to satisfactorily treat SLE. SIRT1 deficiency results in the development of an autoimmune syndrome in mice, including a high titer of anti-nuclear antibody in serum, immunoglobulin deposition in the kidney, and immune complex glomerulonephritis. Resveratrol is an activator of SIRT1 and possesses anti-inflammation and immune-regulatory properties.

Objective: To evaluate the preventative effects of resveratrol on a pristane-induced lupus animal model and assess its putative immune modulation effects.

Methods: BALB/c mice received a single intraperitoneal injection of 0.5 ml of pristane on day 1 and then various doses of resveratrol were given to the mice daily starting on day 2 and continuing for seven months. The autoantibodies in serum and supernatants were measured. Single cells isolated from spleen, isolated CD4+ T cells, and CD19+ B cells were cultured with or without resveratrol in vitro and assessed by flow cytometry.

Results: Resveratrol attenuated proteinuria, immunoglobuin depositon in kidney, and glomerulonephritis as well as IgG1 and IgG2a in serum in pristane-induced lupus mice. Resveratrol also suppressed CD69 and CD71 expression on CD4+ T cells as well as CD4+ T cell proliferation, induced CD4+ T cell apoptosis, and decreased CD4 IFNγ+ Th1 cells and the ratio of Th1/Th2 cells in vitro. In vitro antibody production and proliferation of B cells were also inhibited.

Conclusion: Resveratrol possesses protective effects in pristane-induced lupus mice and may represent a novel approach for the management of SLE.

Show MeSH
Related in: MedlinePlus