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Determination of ploidy level and isolation of genes encoding acetyl-CoA carboxylase in Japanese Foxtail (Alopecurus japonicus).

Xu H, Zhang W, Zhang T, Li J, Wu X, Dong L - PLoS ONE (2014)

Bottom Line: Ploidy level is important in biodiversity studies and in developing strategies for isolating important plant genes.We found that there are two copies of the gene encoding plastidic acetyl-CoA carboxylase (ACCase) in Japanese foxtail and all the homologous genes are expressed.Additionally, no difference in ploidy levels or ACCase gene copy numbers was observed between an ACCase-inhibiting herbicide-resistant and a herbicide-sensitive population in this study.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing, China; Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Beijing, China.

ABSTRACT
Ploidy level is important in biodiversity studies and in developing strategies for isolating important plant genes. Many herbicide-resistant weed species are polyploids, but our understanding of these polyploid weeds is limited. Japanese foxtail, a noxious agricultural grass weed, has evolved herbicide resistance. However, most studies on this weed have ignored the fact that there are multiple copies of target genes. This may complicate the study of resistance mechanisms. Japanese foxtail was found to be a tetraploid by flow cytometer and chromosome counting, two commonly used methods in the determination of ploidy levels. We found that there are two copies of the gene encoding plastidic acetyl-CoA carboxylase (ACCase) in Japanese foxtail and all the homologous genes are expressed. Additionally, no difference in ploidy levels or ACCase gene copy numbers was observed between an ACCase-inhibiting herbicide-resistant and a herbicide-sensitive population in this study.

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Flow cytometric analysis of homogenates prepared from perennial ryegrass (a) and Japanese foxtail (b).
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pone-0114712-g001: Flow cytometric analysis of homogenates prepared from perennial ryegrass (a) and Japanese foxtail (b).

Mentions: The mean fluorescence value of the G0/G1 peak positions for the reference standard was 9.84×105. The mean fluorescence values of the G0/G1 peak positions for the R and S samples were 1.01×106 and 1.00×106, respectively. The coefficient of variation of the cytometry values, as a parameter of the reliability of the measurement, varied between 2.3% and 4.7%. These values were considered acceptable [32]. The flow cytometry results revealed that all these samples were tetraploid. Thus, Japanese foxtail is a tetraploid grass weed species (Fig. 1).


Determination of ploidy level and isolation of genes encoding acetyl-CoA carboxylase in Japanese Foxtail (Alopecurus japonicus).

Xu H, Zhang W, Zhang T, Li J, Wu X, Dong L - PLoS ONE (2014)

Flow cytometric analysis of homogenates prepared from perennial ryegrass (a) and Japanese foxtail (b).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263612&req=5

pone-0114712-g001: Flow cytometric analysis of homogenates prepared from perennial ryegrass (a) and Japanese foxtail (b).
Mentions: The mean fluorescence value of the G0/G1 peak positions for the reference standard was 9.84×105. The mean fluorescence values of the G0/G1 peak positions for the R and S samples were 1.01×106 and 1.00×106, respectively. The coefficient of variation of the cytometry values, as a parameter of the reliability of the measurement, varied between 2.3% and 4.7%. These values were considered acceptable [32]. The flow cytometry results revealed that all these samples were tetraploid. Thus, Japanese foxtail is a tetraploid grass weed species (Fig. 1).

Bottom Line: Ploidy level is important in biodiversity studies and in developing strategies for isolating important plant genes.We found that there are two copies of the gene encoding plastidic acetyl-CoA carboxylase (ACCase) in Japanese foxtail and all the homologous genes are expressed.Additionally, no difference in ploidy levels or ACCase gene copy numbers was observed between an ACCase-inhibiting herbicide-resistant and a herbicide-sensitive population in this study.

View Article: PubMed Central - PubMed

Affiliation: College of Plant Protection, Nanjing Agricultural University, Nanjing, China; Key Laboratory of Integrated Pest Management on Crops in East China (Nanjing Agricultural University), Ministry of Agriculture, Beijing, China.

ABSTRACT
Ploidy level is important in biodiversity studies and in developing strategies for isolating important plant genes. Many herbicide-resistant weed species are polyploids, but our understanding of these polyploid weeds is limited. Japanese foxtail, a noxious agricultural grass weed, has evolved herbicide resistance. However, most studies on this weed have ignored the fact that there are multiple copies of target genes. This may complicate the study of resistance mechanisms. Japanese foxtail was found to be a tetraploid by flow cytometer and chromosome counting, two commonly used methods in the determination of ploidy levels. We found that there are two copies of the gene encoding plastidic acetyl-CoA carboxylase (ACCase) in Japanese foxtail and all the homologous genes are expressed. Additionally, no difference in ploidy levels or ACCase gene copy numbers was observed between an ACCase-inhibiting herbicide-resistant and a herbicide-sensitive population in this study.

Show MeSH
Related in: MedlinePlus