Derivation of ligands for the complement C3a receptor from the C-terminus of C5a.
Bottom Line: However, functional screening in human monocyte-derived macrophages using the xCELLigence label-free platform demonstrated altered specificity of our ligands.This was verified in the presence of C3a receptor antagonist SB 290157 and in a stable cell line expressing either C5a1 or C3a receptor alone.C3a agonism has been suggested to be a potential treatment of acute neutrophil-driven traumatic pathologies, and may have great potential as a therapeutic avenue in this arena.
Affiliation: Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.Show MeSH
Related in: MedlinePlus
Mentions: The 61 peptides, initially dissolved in dimethyl sulfoxide (DMSO) at 10 mM, were screened at a single dose of 8 μM on HMDM using an impedance based label-free system, the xCELLigence; DMSO at <0.5% was found to have no effects in this assay. Fig. 1A shows the identification of 5 significant hits (P<0.001), peptides 20, 31, 47, 48 and 54. Peptide 49 was identified as a potential hit from independent data (data not shown) despite not evoking a response in the initial screen and so was retested at the full dose range along with the five identified hits (see Fig. 1B). Interestingly, a full dose response could not be obtained for peptide 48 (data not shown). However, peptide 49 showed weak activity, despite failing in the initial single dose screen. The EC50 of the hits has been summarized in Table 1.
Affiliation: Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia.