Limits...
Molecular analysis of Colletotrichum species in the carposphere and phyllosphere of olive.

Mosca S, Li Destri Nicosia MG, Cacciola SO, Schena L - PLoS ONE (2014)

Bottom Line: Colletotrichum godetiae and C. acutatum s.s. were by far the most abundant while C. gloeosporioides s.s. was detected in a limited number of samples whereas ther phylotypes were rarely found.As regards to the phenological phase, Colletotrichum species were found in a few samples in June and were diffused on all assessed samples in December.The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by Colletotrichum species in many different pathosystems.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy.

ABSTRACT
A metagenomic approach based on the use of genus specific primers was developed and utilized to characterize Colletotrichum species associated with the olive phyllosphere and carposphere. Selected markers enabled the specific amplification of almost the entire ITS1-5.8S-ITS2 region of the rDNA and its use as barcode gene. The analysis of different olive samples (green and senescent leaves, floral residues, symptomatic and asymptomatic fruits, and litter leaves and mummies) in three different phenological phases (June, October and December) enabled the detection of 12 genotypes associated with 4 phylotypes identified as C. godetiae, C. acutatum s.s., C. gloeosporioides s.s. and C. kahawae. Another three genotypes were not identified at the level of species but were associated with the species complexes of C. acutatum, C. gloeosporioides and C. boninense sensu lato. Colletotrichum godetiae and C. acutatum s.s. were by far the most abundant while C. gloeosporioides s.s. was detected in a limited number of samples whereas ther phylotypes were rarely found. The high incidence of C. acutatum s.s. represents a novelty for Italy and more generally for the Mediterranean basin since it had been previously reported only in Portugal. As regards to the phenological phase, Colletotrichum species were found in a few samples in June and were diffused on all assessed samples in December. According to data new infections on olive tissues mainly occur in the late fall. Furthermore, Colletotrichum species seem to have a saprophytic behavior on floral olive residues. The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by Colletotrichum species in many different pathosystems.

Show MeSH

Related in: MedlinePlus

Phylogenetic trees built using unique sequences representative of all detected genotypes (♦) together with sequences of reference isolates of Colletotrichum acutatum sensu lato[1], C. gloeosporioides s.l.[2] and C. boninense s.l.[3].Genotypes were identified as C. godetiae (A), C. acutatum s.s. (C), C. gloeosporioides s.s. (D) and C. kahawae (F). Three additional genotypes were associated with 2 (B), 6 (D) and 3 (F) species within C. acutatum s.l., C. gloeosporioides s.l and C. boninense s.l., respectively. Separate analyses were conducted for each species complex. Numbers on nodes represent the posterior probabilities for the maximum likelihood method.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4263604&req=5

pone-0114031-g001: Phylogenetic trees built using unique sequences representative of all detected genotypes (♦) together with sequences of reference isolates of Colletotrichum acutatum sensu lato[1], C. gloeosporioides s.l.[2] and C. boninense s.l.[3].Genotypes were identified as C. godetiae (A), C. acutatum s.s. (C), C. gloeosporioides s.s. (D) and C. kahawae (F). Three additional genotypes were associated with 2 (B), 6 (D) and 3 (F) species within C. acutatum s.l., C. gloeosporioides s.l and C. boninense s.l., respectively. Separate analyses were conducted for each species complex. Numbers on nodes represent the posterior probabilities for the maximum likelihood method.

Mentions: The number of samples and the orchards (Cfr. Table 2) in which each genotype was detected is reported together with GenBank accession numbers for sequences. Genotypes were grouped according to their phylogenetic identification (Cfr. Fig. 1).


Molecular analysis of Colletotrichum species in the carposphere and phyllosphere of olive.

Mosca S, Li Destri Nicosia MG, Cacciola SO, Schena L - PLoS ONE (2014)

Phylogenetic trees built using unique sequences representative of all detected genotypes (♦) together with sequences of reference isolates of Colletotrichum acutatum sensu lato[1], C. gloeosporioides s.l.[2] and C. boninense s.l.[3].Genotypes were identified as C. godetiae (A), C. acutatum s.s. (C), C. gloeosporioides s.s. (D) and C. kahawae (F). Three additional genotypes were associated with 2 (B), 6 (D) and 3 (F) species within C. acutatum s.l., C. gloeosporioides s.l and C. boninense s.l., respectively. Separate analyses were conducted for each species complex. Numbers on nodes represent the posterior probabilities for the maximum likelihood method.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263604&req=5

pone-0114031-g001: Phylogenetic trees built using unique sequences representative of all detected genotypes (♦) together with sequences of reference isolates of Colletotrichum acutatum sensu lato[1], C. gloeosporioides s.l.[2] and C. boninense s.l.[3].Genotypes were identified as C. godetiae (A), C. acutatum s.s. (C), C. gloeosporioides s.s. (D) and C. kahawae (F). Three additional genotypes were associated with 2 (B), 6 (D) and 3 (F) species within C. acutatum s.l., C. gloeosporioides s.l and C. boninense s.l., respectively. Separate analyses were conducted for each species complex. Numbers on nodes represent the posterior probabilities for the maximum likelihood method.
Mentions: The number of samples and the orchards (Cfr. Table 2) in which each genotype was detected is reported together with GenBank accession numbers for sequences. Genotypes were grouped according to their phylogenetic identification (Cfr. Fig. 1).

Bottom Line: Colletotrichum godetiae and C. acutatum s.s. were by far the most abundant while C. gloeosporioides s.s. was detected in a limited number of samples whereas ther phylotypes were rarely found.As regards to the phenological phase, Colletotrichum species were found in a few samples in June and were diffused on all assessed samples in December.The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by Colletotrichum species in many different pathosystems.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Agraria, Università Mediterranea, Reggio Calabria, Italy.

ABSTRACT
A metagenomic approach based on the use of genus specific primers was developed and utilized to characterize Colletotrichum species associated with the olive phyllosphere and carposphere. Selected markers enabled the specific amplification of almost the entire ITS1-5.8S-ITS2 region of the rDNA and its use as barcode gene. The analysis of different olive samples (green and senescent leaves, floral residues, symptomatic and asymptomatic fruits, and litter leaves and mummies) in three different phenological phases (June, October and December) enabled the detection of 12 genotypes associated with 4 phylotypes identified as C. godetiae, C. acutatum s.s., C. gloeosporioides s.s. and C. kahawae. Another three genotypes were not identified at the level of species but were associated with the species complexes of C. acutatum, C. gloeosporioides and C. boninense sensu lato. Colletotrichum godetiae and C. acutatum s.s. were by far the most abundant while C. gloeosporioides s.s. was detected in a limited number of samples whereas ther phylotypes were rarely found. The high incidence of C. acutatum s.s. represents a novelty for Italy and more generally for the Mediterranean basin since it had been previously reported only in Portugal. As regards to the phenological phase, Colletotrichum species were found in a few samples in June and were diffused on all assessed samples in December. According to data new infections on olive tissues mainly occur in the late fall. Furthermore, Colletotrichum species seem to have a saprophytic behavior on floral olive residues. The method developed in the present study proved to be valuable and its future application may contribute to the study of cycle and aetiology of diseases caused by Colletotrichum species in many different pathosystems.

Show MeSH
Related in: MedlinePlus