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Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus

Experimental reflectivity vs. internal angle, θ, using the set-up depicted in Figure 2: circles—after incubation of the sample in 10 mM of 11-MUA for 24 h; diamonds—after immobilization of rabbit anti-estrone polyclonal IgG 1:10; triangles—after adding 0.0042 gr estrone in 1 mL DI. Calculated approximations for each signal are shown as smooth curves.
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biosensors-03-00157-f005: Experimental reflectivity vs. internal angle, θ, using the set-up depicted in Figure 2: circles—after incubation of the sample in 10 mM of 11-MUA for 24 h; diamonds—after immobilization of rabbit anti-estrone polyclonal IgG 1:10; triangles—after adding 0.0042 gr estrone in 1 mL DI. Calculated approximations for each signal are shown as smooth curves.

Mentions: The silver surface was modified with 10 mM 11-mercaptoundecanoic acid (11-MUA) dissolved in DMSO. DCC and NHS were added to make the surface reactive, as was described in Section 2. Rabbit anti-estrone polyclonal IgG was then conjugated, making an ester bond with 11-MUA. As shown in Figure 5, the binding of rabbit anti-estrone polyclonal IgG to 11-MUA caused the SPR angle to increase by 2.87°. Further addition of estrone to rabbit anti-estrone polyclonal IgG caused the SPR angle to increase by another 0.95°. The change of the SPR angle indicates the presence of the conjugated material.


Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Experimental reflectivity vs. internal angle, θ, using the set-up depicted in Figure 2: circles—after incubation of the sample in 10 mM of 11-MUA for 24 h; diamonds—after immobilization of rabbit anti-estrone polyclonal IgG 1:10; triangles—after adding 0.0042 gr estrone in 1 mL DI. Calculated approximations for each signal are shown as smooth curves.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263594&req=5

biosensors-03-00157-f005: Experimental reflectivity vs. internal angle, θ, using the set-up depicted in Figure 2: circles—after incubation of the sample in 10 mM of 11-MUA for 24 h; diamonds—after immobilization of rabbit anti-estrone polyclonal IgG 1:10; triangles—after adding 0.0042 gr estrone in 1 mL DI. Calculated approximations for each signal are shown as smooth curves.
Mentions: The silver surface was modified with 10 mM 11-mercaptoundecanoic acid (11-MUA) dissolved in DMSO. DCC and NHS were added to make the surface reactive, as was described in Section 2. Rabbit anti-estrone polyclonal IgG was then conjugated, making an ester bond with 11-MUA. As shown in Figure 5, the binding of rabbit anti-estrone polyclonal IgG to 11-MUA caused the SPR angle to increase by 2.87°. Further addition of estrone to rabbit anti-estrone polyclonal IgG caused the SPR angle to increase by another 0.95°. The change of the SPR angle indicates the presence of the conjugated material.

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus