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Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus

Samples after immobilization of luminescent horseradish peroxidase (HRP) antibody. The samples were immersed in 1 mM (left) and 10 mM (right) of 11-MUA for 24 h, and NHS with EDC was added prior the immobilization.
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biosensors-03-00157-f004: Samples after immobilization of luminescent horseradish peroxidase (HRP) antibody. The samples were immersed in 1 mM (left) and 10 mM (right) of 11-MUA for 24 h, and NHS with EDC was added prior the immobilization.

Mentions: Table 2 implies that luminescence and SPR signals were achieved after immobilization of luminescent HRP antibody, while prior to the immobilization, the samples were incubated in the 10 mM 11-MUA for the period of 12 h, 24 h and 48 h. Therefore, the immobilization succeeded, while in the case of the incubation in 1 mM 11-MUA, the silver experienced major damage, as is documented by the camera and shown in Figure 4(left) together with the sample that was incubated for 12 h. However, there were some damaged areas on the surfaces of the silver that were immersed in 10 mM 11-MUA. In addition, after immersion and washing the samples with ethanol, a layer of aggregates was observed on top of the silver, as is demonstrated in Figure 4(right). This occurred because the Ethanol contains OH group which is known to form a stable bond with silver. Moreover, the affinity of the OH group to the silver is higher than those of thiol, therefore OH binds to Ag and forms visible aggregates. The luminescence and SPR signals were measured on the most smooth and undamaged areas of the samples.


Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Samples after immobilization of luminescent horseradish peroxidase (HRP) antibody. The samples were immersed in 1 mM (left) and 10 mM (right) of 11-MUA for 24 h, and NHS with EDC was added prior the immobilization.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263594&req=5

biosensors-03-00157-f004: Samples after immobilization of luminescent horseradish peroxidase (HRP) antibody. The samples were immersed in 1 mM (left) and 10 mM (right) of 11-MUA for 24 h, and NHS with EDC was added prior the immobilization.
Mentions: Table 2 implies that luminescence and SPR signals were achieved after immobilization of luminescent HRP antibody, while prior to the immobilization, the samples were incubated in the 10 mM 11-MUA for the period of 12 h, 24 h and 48 h. Therefore, the immobilization succeeded, while in the case of the incubation in 1 mM 11-MUA, the silver experienced major damage, as is documented by the camera and shown in Figure 4(left) together with the sample that was incubated for 12 h. However, there were some damaged areas on the surfaces of the silver that were immersed in 10 mM 11-MUA. In addition, after immersion and washing the samples with ethanol, a layer of aggregates was observed on top of the silver, as is demonstrated in Figure 4(right). This occurred because the Ethanol contains OH group which is known to form a stable bond with silver. Moreover, the affinity of the OH group to the silver is higher than those of thiol, therefore OH binds to Ag and forms visible aggregates. The luminescence and SPR signals were measured on the most smooth and undamaged areas of the samples.

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus