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Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus

Prism coupling sensor and the diverged beam surface plasmon resonance (SPR) imaging (DBSPRI) arrangement scheme for improved angular interrogation without mechanical scanning.
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biosensors-03-00157-f003: Prism coupling sensor and the diverged beam surface plasmon resonance (SPR) imaging (DBSPRI) arrangement scheme for improved angular interrogation without mechanical scanning.

Mentions: Quasi-monochromatic laser light of wavelength 637 nm from the focusable laser diode was coupled to the plasmon modes at the metal-analyte interface with a variety of incident angels using a diverging incident light beam, which is in the z-x plan in the KR configuration by using a right angle BK7 or SF11 prism, as is shown in Figure 3 and explained thoroughly in our previous publications (see Karabchevsky et al.) [24,32]. The evanescent wave is generated at a single incidence-angle within the angular range of the diverged transverse-magnetic (TM) polarized beam. Reflected light is captured by the 659 × 494 pixels CMOS camera. When the momentum of the photons matches the momentum of the metal electrons (Equation (1) is satisfied), the dark line appears on the bright background of the captured images, which corresponds to the excitation of the surface plasmon.


Study of Immobilization Procedure on Silver Nanolayers and Detection of Estrone with Diverged Beam Surface Plasmon Resonance (SPR) Imaging.

Karabchevsky A, Tsapovsky L, Marks RS, Abdulhalim I - Biosensors (Basel) (2013)

Prism coupling sensor and the diverged beam surface plasmon resonance (SPR) imaging (DBSPRI) arrangement scheme for improved angular interrogation without mechanical scanning.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263594&req=5

biosensors-03-00157-f003: Prism coupling sensor and the diverged beam surface plasmon resonance (SPR) imaging (DBSPRI) arrangement scheme for improved angular interrogation without mechanical scanning.
Mentions: Quasi-monochromatic laser light of wavelength 637 nm from the focusable laser diode was coupled to the plasmon modes at the metal-analyte interface with a variety of incident angels using a diverging incident light beam, which is in the z-x plan in the KR configuration by using a right angle BK7 or SF11 prism, as is shown in Figure 3 and explained thoroughly in our previous publications (see Karabchevsky et al.) [24,32]. The evanescent wave is generated at a single incidence-angle within the angular range of the diverged transverse-magnetic (TM) polarized beam. Reflected light is captured by the 659 × 494 pixels CMOS camera. When the momentum of the photons matches the momentum of the metal electrons (Equation (1) is satisfied), the dark line appears on the bright background of the captured images, which corresponds to the excitation of the surface plasmon.

Bottom Line: The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques.To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass.At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

View Article: PubMed Central - PubMed

Affiliation: Electro-Optic Engineering Unit and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel; E-Mail: abdulhlm@bgu.ac.il.

ABSTRACT
An immobilization protocol was developed to attach receptors on smooth silver thin films. Dense and packed 11-mercaptoundecanoic acid (11-MUA) was used to avoid uncontrolled sulfidization and harmful oxidation of silver nanolayers. N,N'-dicyclohexylcarbodiimide (DCC) and N-hydroxysuccinimide (NHS) were added to make the silver surfaces reactive. A comparative study was carried out with different immersion times of silver samples in 11-MUA solutions with different concentrations to find the optimum conditions for immobilization. The signals, during each step of the protocol, were analyzed with a refractometer based on the surface plasmon resonance (SPR) effect and luminescence techniques. Molecular interactions at the surfaces between the probe and target at the surface nanolayer shift the SPR signal, thus indicating the presence of the substance. To demonstrate specific biosensing, rabbit anti-estrone polyclonal immunoglobulin G (IgG) antibody was immobilized through a linker on 47 nm silver layer deposited on SF11 glass. At the final stage, the representative endocrine disruptor-estrone-was attached and detected in deionized water with a diverging beam SPR imaging sensor.

No MeSH data available.


Related in: MedlinePlus