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Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.


Immunosensor principle based on the cross-reactivity of an antibody. Example of atrazine (ATZ) detection [47].
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biosensors-03-00058-f014: Immunosensor principle based on the cross-reactivity of an antibody. Example of atrazine (ATZ) detection [47].

Mentions: After formation of the HATZ/αATZ complex, the faradic current of the quinone group decreases (Figure 14, step 2). The electrode modified by poly(JUG-HATZ) where αATZ is complexed is then utilized to detect ATZ in solution. Indeed, αATZ preferentially binds to ATZ, so that a displacement equilibrium occurs (Figure 14, step 3) between ATZ in solution and HATZ incorporated in the polymer. Addition of free atrazine removes the complexed antibodies from the electrode surface leading to an increase in electroactivity. It is proposed that the removal of the αATZ enhances the ionic flux through the interface and leads to this current increase. Here, the relative projected area between probe and target is (150/0.3)2/3 = 63, even higher than for peptide probes (see Section 2.2.3), which may explain the high sensitivity of this architecture.


Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Immunosensor principle based on the cross-reactivity of an antibody. Example of atrazine (ATZ) detection [47].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263589&req=5

biosensors-03-00058-f014: Immunosensor principle based on the cross-reactivity of an antibody. Example of atrazine (ATZ) detection [47].
Mentions: After formation of the HATZ/αATZ complex, the faradic current of the quinone group decreases (Figure 14, step 2). The electrode modified by poly(JUG-HATZ) where αATZ is complexed is then utilized to detect ATZ in solution. Indeed, αATZ preferentially binds to ATZ, so that a displacement equilibrium occurs (Figure 14, step 3) between ATZ in solution and HATZ incorporated in the polymer. Addition of free atrazine removes the complexed antibodies from the electrode surface leading to an increase in electroactivity. It is proposed that the removal of the αATZ enhances the ionic flux through the interface and leads to this current increase. Here, the relative projected area between probe and target is (150/0.3)2/3 = 63, even higher than for peptide probes (see Section 2.2.3), which may explain the high sensitivity of this architecture.

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.