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Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.


(a) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-HPV (curve c). (b) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-OVA (curve c) [42].
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biosensors-03-00058-f011: (a) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-HPV (curve c). (b) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-OVA (curve c) [42].

Mentions: SWV was used to characterize the presence of the antibody. As expected, formation of the HPV-16-L1/anti-HPV complex induces a strong decrease in the polymer electroactivity (Figure 11(a)). If the same experiment is performed with a non-target antibody (e.g., anti-OVA), the current drop is lower than for anti-HPV (Figure 11(b)), which demonstrates the specificity of the molecular recognition.


Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

(a) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-HPV (curve c). (b) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-OVA (curve c) [42].
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263589&req=5

biosensors-03-00058-f011: (a) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-HPV (curve c). (b) SWV of a poly(HNQ-co-HSNQA) recorded in PBS before grafting (curve a), after grafting of 5 × 10−8 M HPV-16-L1 (curve b) and after complexation with 5 × 10−8 M α-OVA (curve c) [42].
Mentions: SWV was used to characterize the presence of the antibody. As expected, formation of the HPV-16-L1/anti-HPV complex induces a strong decrease in the polymer electroactivity (Figure 11(a)). If the same experiment is performed with a non-target antibody (e.g., anti-OVA), the current drop is lower than for anti-HPV (Figure 11(b)), which demonstrates the specificity of the molecular recognition.

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.