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Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.


Structure of glutathione.
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biosensors-03-00058-f008: Structure of glutathione.

Mentions: The α-Amino acids can bind to each other through an amide bond between the α-carboxyl group of one and the α-amine group of another, to form peptides. Compared to proteins, peptides are smaller, structurally less complex and more stable. Their synthesis can be automated, which is a considerable advantage compared to proteins and allows new biological and biomedical applications, as well as for diagnostics. Among other peptides of interest, glutathione (γ-L-glutamyl-L-cysteinyl-glycine) is potentially an efficient linker for biomolecule immobilization on the surface. Indeed, it is a tripeptide, bearing one primary amine, two amide groups, two carboxylic groups and one thiol group, all these groups being chemically reactive (see Figure 8).


Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Structure of glutathione.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263589&req=5

biosensors-03-00058-f008: Structure of glutathione.
Mentions: The α-Amino acids can bind to each other through an amide bond between the α-carboxyl group of one and the α-amine group of another, to form peptides. Compared to proteins, peptides are smaller, structurally less complex and more stable. Their synthesis can be automated, which is a considerable advantage compared to proteins and allows new biological and biomedical applications, as well as for diagnostics. Among other peptides of interest, glutathione (γ-L-glutamyl-L-cysteinyl-glycine) is potentially an efficient linker for biomolecule immobilization on the surface. Indeed, it is a tripeptide, bearing one primary amine, two amide groups, two carboxylic groups and one thiol group, all these groups being chemically reactive (see Figure 8).

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.