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Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.


(a) Structure of 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone) (HSNQA) and (b) 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA).
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biosensors-03-00058-f004: (a) Structure of 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone) (HSNQA) and (b) 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA).

Mentions: We first synthesized 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone.(HSNQA) (Figure 4(a)). The reaction of thiols with various hydroxynaphthoquinone derivatives leads, in one step, to substituted quinone rings, under mild conditions [49]. Another spacer was also designed by straightforward carbon-carbon coupling, leading to 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA) (Figure 4(b)) [42].


Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

(a) Structure of 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone) (HSNQA) and (b) 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263589&req=5

biosensors-03-00058-f004: (a) Structure of 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone) (HSNQA) and (b) 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA).
Mentions: We first synthesized 5-hydroxy-2-thioacetic acid-1,4-naphthoquinone.(HSNQA) (Figure 4(a)). The reaction of thiols with various hydroxynaphthoquinone derivatives leads, in one step, to substituted quinone rings, under mild conditions [49]. Another spacer was also designed by straightforward carbon-carbon coupling, leading to 5-hydroxy-1,4-naphthoquinone-3-propionic acid (HNQA) (Figure 4(b)) [42].

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.