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Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.


Principle of enzyme-linked immunosorbent assay (ELISA). (a) Sandwich format (b) Competitive format.
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biosensors-03-00058-f001: Principle of enzyme-linked immunosorbent assay (ELISA). (a) Sandwich format (b) Competitive format.

Mentions: Analytical tools using Ab-Ag couples are called immunoassays. They are capable of direct and specific detection in complex samples, due to highly specific interactions between Ab and Ag. Commercially available immunoassay kits are inexpensive, simple, adapted to field use and constitute a rapid way of determining contaminants in environmental samples. Generally, the detection scheme relies on labeling with radioisotopes (radioimmunoassay, RIA, now scarcely used), chemiluminescent compounds (chemiluminescence immunoassay, CLIA) [1,2] or enzymes (enzyme immunoassay, EIA, and enzyme-linked immunosorbent assay, ELISA) [3,4,5,6]. Usually, the Ab or the Ag is immobilized on a substrate. In the most basic format, an immunocomplex (Ab-Ag) is formed upon contact with a solution containing the labeled analyte (generally the Ag). The analyte concentration in solution is then derived from that of bound analyte. In a more efficient sandwich format, Ab are immobilized on a substrate which is put in contact with the sample containing the Ag, then a labeled secondary antibody is added (see Figure 1(a)). Competitive formats also exist, in which competition takes place between non-labeled and labeled Ag for an immobilized Ab (Figure 1(b)).


Quinone-based polymers for label-free and reagentless electrochemical immunosensors: application to proteins, antibodies and pesticides detection.

Piro B, Reisberg S, Anquetin G, Duc HT, Pham MC - Biosensors (Basel) (2013)

Principle of enzyme-linked immunosorbent assay (ELISA). (a) Sandwich format (b) Competitive format.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263589&req=5

biosensors-03-00058-f001: Principle of enzyme-linked immunosorbent assay (ELISA). (a) Sandwich format (b) Competitive format.
Mentions: Analytical tools using Ab-Ag couples are called immunoassays. They are capable of direct and specific detection in complex samples, due to highly specific interactions between Ab and Ag. Commercially available immunoassay kits are inexpensive, simple, adapted to field use and constitute a rapid way of determining contaminants in environmental samples. Generally, the detection scheme relies on labeling with radioisotopes (radioimmunoassay, RIA, now scarcely used), chemiluminescent compounds (chemiluminescence immunoassay, CLIA) [1,2] or enzymes (enzyme immunoassay, EIA, and enzyme-linked immunosorbent assay, ELISA) [3,4,5,6]. Usually, the Ab or the Ag is immobilized on a substrate. In the most basic format, an immunocomplex (Ab-Ag) is formed upon contact with a solution containing the labeled analyte (generally the Ag). The analyte concentration in solution is then derived from that of bound analyte. In a more efficient sandwich format, Ab are immobilized on a substrate which is put in contact with the sample containing the Ag, then a labeled secondary antibody is added (see Figure 1(a)). Competitive formats also exist, in which competition takes place between non-labeled and labeled Ag for an immobilized Ab (Figure 1(b)).

Bottom Line: Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors.Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format.Herein, these developments are briefly reviewed and put into perspective.

View Article: PubMed Central - PubMed

Affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France; E-Mails: steeve.reisberg@univ-paris-diderot.fr (S.R.); guillaume.anquetin@univ-paris-diderot.fr (G.A.); mcpham@univ-paris-diderot.fr (M.-C.P.).

ABSTRACT
Polyquinone derivatives are widely recognized in the literature for their remarkable properties, their biocompatibility, simple synthesis, and easy bio-functionalization. We have shown that polyquinones present very stable electroactivity in neutral aqueous medium within the cathodic potential domain avoiding side oxidation of interfering species. Besides, they can act as immobilized redox transducers for probing biomolecular interactions in sensors. Our group has been working on devices based on such modified electrodes with a view to applications for proteins, antibodies and organic pollutants using a reagentless label-free electrochemical immunosensor format. Herein, these developments are briefly reviewed and put into perspective.

No MeSH data available.