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Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.


(A) A line profile over a single globular object in the AFM topograph after the immobilization of streptavidin. The inset shows the AFM image (200 nm × 200 nm, height scale 6 nm) with a line indicating the location of line profile. (B) A line profile over a single globular object in the AFM topograph after the immobilization of bio-CRP antigen. The inset shows the AFM image (200 nm × 200 nm, height scale 8 nm) with a line indicating the location of line profile. (C) A line profile over a single globular object in the AFM topograph after the immobilization of CRP antibody. The inset shows the AFM image (200 nm × 200 nm, height scale 19 nm) with a line indicating the location of line profile.
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biosensors-03-00001-f009: (A) A line profile over a single globular object in the AFM topograph after the immobilization of streptavidin. The inset shows the AFM image (200 nm × 200 nm, height scale 6 nm) with a line indicating the location of line profile. (B) A line profile over a single globular object in the AFM topograph after the immobilization of bio-CRP antigen. The inset shows the AFM image (200 nm × 200 nm, height scale 8 nm) with a line indicating the location of line profile. (C) A line profile over a single globular object in the AFM topograph after the immobilization of CRP antibody. The inset shows the AFM image (200 nm × 200 nm, height scale 19 nm) with a line indicating the location of line profile.


Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

(A) A line profile over a single globular object in the AFM topograph after the immobilization of streptavidin. The inset shows the AFM image (200 nm × 200 nm, height scale 6 nm) with a line indicating the location of line profile. (B) A line profile over a single globular object in the AFM topograph after the immobilization of bio-CRP antigen. The inset shows the AFM image (200 nm × 200 nm, height scale 8 nm) with a line indicating the location of line profile. (C) A line profile over a single globular object in the AFM topograph after the immobilization of CRP antibody. The inset shows the AFM image (200 nm × 200 nm, height scale 19 nm) with a line indicating the location of line profile.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263588&req=5

biosensors-03-00001-f009: (A) A line profile over a single globular object in the AFM topograph after the immobilization of streptavidin. The inset shows the AFM image (200 nm × 200 nm, height scale 6 nm) with a line indicating the location of line profile. (B) A line profile over a single globular object in the AFM topograph after the immobilization of bio-CRP antigen. The inset shows the AFM image (200 nm × 200 nm, height scale 8 nm) with a line indicating the location of line profile. (C) A line profile over a single globular object in the AFM topograph after the immobilization of CRP antibody. The inset shows the AFM image (200 nm × 200 nm, height scale 19 nm) with a line indicating the location of line profile.
Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.