Limits...
Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.


X-ray photoelectron spectroscopy (XPS) N1s peaks for (A) gold electrode (B) MuOH:Biotin-PEG-thiol (85:15 mol%) SAM, (C) streptavidin, (D) bio-CRP antigen and (E) anti-CRP antibody layers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4263588&req=5

biosensors-03-00001-f004: X-ray photoelectron spectroscopy (XPS) N1s peaks for (A) gold electrode (B) MuOH:Biotin-PEG-thiol (85:15 mol%) SAM, (C) streptavidin, (D) bio-CRP antigen and (E) anti-CRP antibody layers.

Mentions: Immobilization of the subsequent protein layers on the paper-supported printed gold electrodes was followed by XPS and AFM. Table 1 lists the XPS elemental composition and Figure 4 shows high resolution spectra for the N1s peak after each immobilization step.


Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

X-ray photoelectron spectroscopy (XPS) N1s peaks for (A) gold electrode (B) MuOH:Biotin-PEG-thiol (85:15 mol%) SAM, (C) streptavidin, (D) bio-CRP antigen and (E) anti-CRP antibody layers.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263588&req=5

biosensors-03-00001-f004: X-ray photoelectron spectroscopy (XPS) N1s peaks for (A) gold electrode (B) MuOH:Biotin-PEG-thiol (85:15 mol%) SAM, (C) streptavidin, (D) bio-CRP antigen and (E) anti-CRP antibody layers.
Mentions: Immobilization of the subsequent protein layers on the paper-supported printed gold electrodes was followed by XPS and AFM. Table 1 lists the XPS elemental composition and Figure 4 shows high resolution spectra for the N1s peak after each immobilization step.

Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.