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Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.


Schematic diagram of the structure (not to scale) of the supramolecular protein layers streptavidin, biotinylated c-reactive protein (CRP) antigen and the bound analyte (anti-CRP antibody) grown on biotinylated self-assembly monolayer (SAM)-covered printed gold electrodes on a paper substrate.
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biosensors-03-00001-f001: Schematic diagram of the structure (not to scale) of the supramolecular protein layers streptavidin, biotinylated c-reactive protein (CRP) antigen and the bound analyte (anti-CRP antibody) grown on biotinylated self-assembly monolayer (SAM)-covered printed gold electrodes on a paper substrate.

Mentions: A schematic representation of the supramolecular protein layers is provided in Figure 1. The first layer consisted of an adsorbed biotinylated self-assembled monolayer (SAM) on the gold electrodes, formed from a MuOH:Biotin-PEG (85:15 mol%, 5 mM) (MBP) thiol solution in absolute ethanol (ETAX Aa, Altia). Before exposure to SAM solution, the gold electrodes were cleaned with plasma (air) flow (PDC-326, Harrick) for 2 min, rinsed with acetone and absolute ethanol and dried with nitrogen gas. The paper-supported electrodes were sealed between two silicon rings in a liquid cell with a cap to prevent evaporation, and the electrode surfaces were exposed to SAM solution (250 µL) for 16 h at room temperature in the dark. The MBP was adsorbed on the gold surfaces. After thiolation, the substrates were removed from the solution and rinsed immediately with absolute ethanol and dried with nitrogen gas. The MBP SAM was used as a starting point for the formation of supramolecular recognition assembly to ensure that the anchored streptavidin molecules remained biologically active and in their native conformations and formed a homogenous and strongly bound intermediate layer with a high binding capacity for subsequent immobilization of bio-CRP.


Application of paper-supported printed gold electrodes for impedimetric immunosensor development.

Ihalainen P, Majumdar H, Viitala T, Törngren B, Närjeoja T, Määttänen A, Sarfraz J, Härmä H, Yliperttula M, Österbacka R, Peltonen J - Biosensors (Basel) (2012)

Schematic diagram of the structure (not to scale) of the supramolecular protein layers streptavidin, biotinylated c-reactive protein (CRP) antigen and the bound analyte (anti-CRP antibody) grown on biotinylated self-assembly monolayer (SAM)-covered printed gold electrodes on a paper substrate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263588&req=5

biosensors-03-00001-f001: Schematic diagram of the structure (not to scale) of the supramolecular protein layers streptavidin, biotinylated c-reactive protein (CRP) antigen and the bound analyte (anti-CRP antibody) grown on biotinylated self-assembly monolayer (SAM)-covered printed gold electrodes on a paper substrate.
Mentions: A schematic representation of the supramolecular protein layers is provided in Figure 1. The first layer consisted of an adsorbed biotinylated self-assembled monolayer (SAM) on the gold electrodes, formed from a MuOH:Biotin-PEG (85:15 mol%, 5 mM) (MBP) thiol solution in absolute ethanol (ETAX Aa, Altia). Before exposure to SAM solution, the gold electrodes were cleaned with plasma (air) flow (PDC-326, Harrick) for 2 min, rinsed with acetone and absolute ethanol and dried with nitrogen gas. The paper-supported electrodes were sealed between two silicon rings in a liquid cell with a cap to prevent evaporation, and the electrode surfaces were exposed to SAM solution (250 µL) for 16 h at room temperature in the dark. The MBP was adsorbed on the gold surfaces. After thiolation, the substrates were removed from the solution and rinsed immediately with absolute ethanol and dried with nitrogen gas. The MBP SAM was used as a starting point for the formation of supramolecular recognition assembly to ensure that the anchored streptavidin molecules remained biologically active and in their native conformations and formed a homogenous and strongly bound intermediate layer with a high binding capacity for subsequent immobilization of bio-CRP.

Bottom Line: In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates.We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique.The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails: bjorn.torngren@abo.fi (B.T.); anni.maattanen@abo.fi (A.M.); jawad.sarfraz@abo.fi (J.S.); jouko.peltonen@abo.fi (J.P.).

ABSTRACT
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

No MeSH data available.