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The effect of 3-thiopheneacetic Acid in the polymerization of a conductive electrotextile for use in biosensor development.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2013)

Bottom Line: The objectives of this study were to determine: (1) if the inclusion of 3TAA in the polymerization process would have an effect on the availability of binding sites in the high-surface area electrotextile for biorecognition elements and (2) how the increase in the concentration of 3TAA would affect the physical characteristics of the coating, resistivity of the sample and availability of binding sites.It was found that the addition of 3TAA to the polymerization process resulted in an increase in the size of the polypyrrole coating, as well as the material resistivity and available binding sites for biorecognition elements.A polymer coated membrane sample containing a concentration within the range of 10-50 mg/mL of 3TAA was selected as the best for future biosensor work.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. shannon.k.mcgraw2.civ@mail.mil.

ABSTRACT
Investigations were conducted to develop an electrotextile using a nonwoven polypropylene fiber platform conformally coated in a conductive, functionalized copolymer of polypyrrole and 3-thiopheneacetic acid (3TAA). The objectives of this study were to determine: (1) if the inclusion of 3TAA in the polymerization process would have an effect on the availability of binding sites in the high-surface area electrotextile for biorecognition elements and (2) how the increase in the concentration of 3TAA would affect the physical characteristics of the coating, resistivity of the sample and availability of binding sites. It was found that the addition of 3TAA to the polymerization process resulted in an increase in the size of the polypyrrole coating, as well as the material resistivity and available binding sites for biorecognition elements. These factors were used to determine which of the tested concentrations was best for biosensor development. A polymer coated membrane sample containing a concentration within the range of 10-50 mg/mL of 3TAA was selected as the best for future biosensor work.

No MeSH data available.


Change in average fluorescent output after FITC-avidin binding based on increasing 3TAA concentrations with error bars showing the standard error of the mean for each sample. The overall trend shows the average fluorescence output increasing at larger concentrations of 3TAA.
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biosensors-03-00286-f005: Change in average fluorescent output after FITC-avidin binding based on increasing 3TAA concentrations with error bars showing the standard error of the mean for each sample. The overall trend shows the average fluorescence output increasing at larger concentrations of 3TAA.

Mentions: The intensity of the FITC signal measured following the crosslinking reaction was used as an indicator of the relative amount of avidin that was successfully attached to the available binding sites provided by the presence of carboxyl groups in the polymer coating. The average fluorescence output for each sample can be seen in Table 1. The average fluorescence signal measured range from 1.0287 to 3.9623 relative fluorescence units (RFUs). Only the sample containing 0 mg/mL of 3TAA measures below the value of 1.1 RFUs. Although the average fluorescent output of the sample containing 0 mg/mL 3TAA was the lowest of all samples measured, there was still an unexpected fluorescent signal. This is most likely due to the FITC-avidin nonspecifically attaching to the rough surface and in the pores between the polymer coated fibers. The samples containing 50 and 100 mg/mL both exceed 1.5 RFUs. The sharpest increase in signal comes between the samples containing 50 and 100 mg/mL, with the difference being 2.317 RFUs. The increase in concentration of 3TAA in each sample coincides with an increase in fluorescent signal for every sample, except between 10 and 20 mg/mL. The relationship between the average fluorescent readout value and concentration of 3TAA in each sample can be seen in Figure 5.


The effect of 3-thiopheneacetic Acid in the polymerization of a conductive electrotextile for use in biosensor development.

McGraw SK, Alocilja E, Senecal A, Senecal K - Biosensors (Basel) (2013)

Change in average fluorescent output after FITC-avidin binding based on increasing 3TAA concentrations with error bars showing the standard error of the mean for each sample. The overall trend shows the average fluorescence output increasing at larger concentrations of 3TAA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263581&req=5

biosensors-03-00286-f005: Change in average fluorescent output after FITC-avidin binding based on increasing 3TAA concentrations with error bars showing the standard error of the mean for each sample. The overall trend shows the average fluorescence output increasing at larger concentrations of 3TAA.
Mentions: The intensity of the FITC signal measured following the crosslinking reaction was used as an indicator of the relative amount of avidin that was successfully attached to the available binding sites provided by the presence of carboxyl groups in the polymer coating. The average fluorescence output for each sample can be seen in Table 1. The average fluorescence signal measured range from 1.0287 to 3.9623 relative fluorescence units (RFUs). Only the sample containing 0 mg/mL of 3TAA measures below the value of 1.1 RFUs. Although the average fluorescent output of the sample containing 0 mg/mL 3TAA was the lowest of all samples measured, there was still an unexpected fluorescent signal. This is most likely due to the FITC-avidin nonspecifically attaching to the rough surface and in the pores between the polymer coated fibers. The samples containing 50 and 100 mg/mL both exceed 1.5 RFUs. The sharpest increase in signal comes between the samples containing 50 and 100 mg/mL, with the difference being 2.317 RFUs. The increase in concentration of 3TAA in each sample coincides with an increase in fluorescent signal for every sample, except between 10 and 20 mg/mL. The relationship between the average fluorescent readout value and concentration of 3TAA in each sample can be seen in Figure 5.

Bottom Line: The objectives of this study were to determine: (1) if the inclusion of 3TAA in the polymerization process would have an effect on the availability of binding sites in the high-surface area electrotextile for biorecognition elements and (2) how the increase in the concentration of 3TAA would affect the physical characteristics of the coating, resistivity of the sample and availability of binding sites.It was found that the addition of 3TAA to the polymerization process resulted in an increase in the size of the polypyrrole coating, as well as the material resistivity and available binding sites for biorecognition elements.A polymer coated membrane sample containing a concentration within the range of 10-50 mg/mL of 3TAA was selected as the best for future biosensor work.

View Article: PubMed Central - PubMed

Affiliation: Biosystems and Agricultural Engineering, Michigan State University, 524 S. Shaw Lane, 115 Farrall Hall, East Lansing, MI 48824, USA. shannon.k.mcgraw2.civ@mail.mil.

ABSTRACT
Investigations were conducted to develop an electrotextile using a nonwoven polypropylene fiber platform conformally coated in a conductive, functionalized copolymer of polypyrrole and 3-thiopheneacetic acid (3TAA). The objectives of this study were to determine: (1) if the inclusion of 3TAA in the polymerization process would have an effect on the availability of binding sites in the high-surface area electrotextile for biorecognition elements and (2) how the increase in the concentration of 3TAA would affect the physical characteristics of the coating, resistivity of the sample and availability of binding sites. It was found that the addition of 3TAA to the polymerization process resulted in an increase in the size of the polypyrrole coating, as well as the material resistivity and available binding sites for biorecognition elements. These factors were used to determine which of the tested concentrations was best for biosensor development. A polymer coated membrane sample containing a concentration within the range of 10-50 mg/mL of 3TAA was selected as the best for future biosensor work.

No MeSH data available.