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A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform.

Xiao N, Wang C, Yu C - Biosensors (Basel) (2013)

Bottom Line: Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform.The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration.The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA. nxiao@iastate.edu.

ABSTRACT
Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

No MeSH data available.


Related in: MedlinePlus

(a) Silver nanocubes used to make molecular probes (STEM image); (b) Reaction scheme to functionalize silver nanocubes with anti-E. coli antibodies.
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biosensors-03-00312-f002: (a) Silver nanocubes used to make molecular probes (STEM image); (b) Reaction scheme to functionalize silver nanocubes with anti-E. coli antibodies.

Mentions: To take advantage of the superior surface enhancement performance of anisotropic nanoparticles due to the stronger local electromagnetic field around the singular features (sharp corners and edges) of these particles [6,20], we chose to use Ag nanocubes (as shown in Figure 2(a)) to make nanoprobes.


A Self-Referencing Detection of Microorganisms Using Surface Enhanced Raman Scattering Nanoprobes in a Test-in-a-Tube Platform.

Xiao N, Wang C, Yu C - Biosensors (Basel) (2013)

(a) Silver nanocubes used to make molecular probes (STEM image); (b) Reaction scheme to functionalize silver nanocubes with anti-E. coli antibodies.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263577&req=5

biosensors-03-00312-f002: (a) Silver nanocubes used to make molecular probes (STEM image); (b) Reaction scheme to functionalize silver nanocubes with anti-E. coli antibodies.
Mentions: To take advantage of the superior surface enhancement performance of anisotropic nanoparticles due to the stronger local electromagnetic field around the singular features (sharp corners and edges) of these particles [6,20], we chose to use Ag nanocubes (as shown in Figure 2(a)) to make nanoprobes.

Bottom Line: Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform.The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration.The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA. nxiao@iastate.edu.

ABSTRACT
Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.

No MeSH data available.


Related in: MedlinePlus